|Cat. No. 122 102||200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.|
WB: 1 : 1000 up to 1 : 20000 (AP staining) gallery
IP: yes (see remarks)
ICC: 1 : 200 up to 1 : 500 gallery
IHC: not tested yet
IHC-P/FFPE: 1 : 200 gallery
|Immunogen||Synthetic peptide corresponding to AA 45 to 81 from mouse Complexin2 (UniProt Id: P84086)|
Reacts with: human (O14810, Q6PUV4), rat (P63041, P84087), mouse (P63040, P84086), Guinea pig, cow, zebrafish, rabbit.
Other species not tested yet.
|Specificity||Recognizes complexin 1 and 2.|
IP: Co-immunoprecipitates the SNARE complex.
Complexins are enriched in neurons where they colocalize with syntaxin 1 and SNAP 25. In addition, complexin 2, also referred to as synaphin 1, is expressed ubiquitously at low levels. Complexins bind weakly to syntaxin 1 alone and not at all to synaptobrevin and SNAP 25, but strongly to the SNAP receptor-core complex composed of these three molecules. They compete with α-SNAP for binding to the core complex but not with other interacting molecules, suggesting that complexins regulate the sequential interactions of α-SNAP and synaptotagmins with the SNAP receptor during exocytosis.
In retinal ribbon synapses complexin 3 and complexin 4 functionally replace complexin 1 (synaphin 2) and 2. They have similar biochemical binding properties and are farnesylated at their C-terminus.