Synaptic Systems - Complexin1/2

Cat. No.: 122 102

Amount: 200 µl

Price: $360.00

Cat. No. 122 102	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 20000 (AP staining) gallery IP: yes (see remarks) ICC: 1 : 200 up to 1 : 500 gallery IHC: yes (see remarks) IHC-P: 1 : 200 gallery
Immunogen	Synthetic peptide corresponding to AA 45 to 81 from mouse Complexin2 (UniProt Id: P84086)
Reactivity	Reacts with: human (O14810, Q6PUV4), rat (P63041, P84087), mouse (P63040, P84086), Guinea pig, cow, zebrafish, rabbit. Other species not tested yet.
Specificity	Recognizes complexin 1 and 2. K.O. validated PubMed: 11163241
Remarks	IP: Co-immunoprecipitates the SNARE complex. Immunogen is located inside the mapped binding domain of complexin 2 to the SNARE complex (aa 41 - 91). IHC: For optimal results, mild fixation (immersion fixation with 4% PFA for 15 min) according to Gierke et al. 2023 is recommended.
Data sheet	122_102.pdf

The proteomic landscape of synaptic diversity across brain regions and cell types.
van Oostrum M, Blok TM, Giandomenico SL, Tom Dieck S, Tushev G, Fürst N, Langer JD, Schuman EM
Cell (2023) 18624: 5411-5427.e23. 122 102 WB; tested species: mouse

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 122 102 WB

Complexins regulate a late step in Ca2+-dependent neurotransmitter release.
Reim K, Mansour M, Varoqueaux F, McMahon HT, Südhof TC, Brose N, Rosenmund C
Cell (2001) 1041: 71-81. 122 102 WB; KO verified; tested species: mouse

Cat. No.: 122 102

Amount: 200 µl

Price: $360.00

ICC

IHC-P

Background

Protocols

Complexin1/2 antibody SNARE binding domain - 122 102 K.O.