Complexin1/2 antibody C-terminus - 122 004 K.O.

Complexins are nerve-terminal syntaxin binding proteins

Guinea pig polyclonal antiserum

Cat. No.: 122 004

Amount: 100 µl

Price: $370.00

Cat. No. 122 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 200 up to 1 : 500 (see remarks) gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 122 to 134 from human Complexin2 (UniProt Id: Q6PUV4)
Reactivity	Reacts with: human (O14810, Q6PUV4), rat (P63041, P84087), mouse (P63040, P84086). Other species not tested yet.
Specificity	Recognizes complexin 1 and 2. K.O. validated PubMed: 34360929
Matching control protein/peptide	122-0P
Remarks	IHC: For optimal results in retina tissue, follow the retina protocol.
Data sheet	Datasheet 122_004

References for Complexin1/2 - 122 004

Cell Types and Synapses Expressing the SNARE Complex Regulating Proteins Complexin 1 and Complexin 2 in Mammalian Retina.
Lux UT, Ehrenberg J, Joachimsthaler A, Atorf J, Pircher B, Reim K, Kremers J, Gießl A, Brandstätter JH
International journal of molecular sciences (2021) 2215: . 122 004 IHC; KO verified; tested species: mouse

Cat. No.: 122 004

Amount: 100 µl

Price: $370.00

ICC

IHC

IHC-P

Background

Complexins are enriched in neurons where they colocalize with syntaxin 1 and SNAP 25. In addition, complexin 2, also referred to as synaphin 1, is expressed ubiquitously at low levels. Complexins bind weakly to syntaxin 1 alone and not at all to synaptobrevin and SNAP 25, but strongly to the SNAP receptor-core complex composed of these three molecules. They compete with α-SNAP for binding to the core complex but not with other interacting molecules, suggesting that complexins regulate the sequential interactions of α-SNAP and synaptotagmins with the SNAP receptor during exocytosis.
In retinal ribbon synapses complexin 3 and complexin 4 functionally replace complexin 1 (synaphin 2) and 2. They have similar biochemical binding properties and are farnesylated at their C-terminus.

1 ) Südhof TC et al., Nature (1995)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) McMahon HT et al., Cell (1995)

Protocols

ICC

Fixed Cells

IHC

IHC-P

Standard Condition

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