|Cat. No. 120 002||200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.|
WB: 1 : 1000 (AP staining) gallery
ICC: 1 : 500 gallery
IHC: 1 : 400 gallery
IHC-P/FFPE: not tested yet
|Immunogen||Synthetic peptide corresponding to AA 2 to 15 from rat Amphiphysin (UniProt Id: O08838)|
Reacts with: human (P49418), rat (O08838), mouse (Q7TQF7), hamster, cow, chicken.
Other species not tested yet.
|Specificity||Specific for amphiphysin.|
|Matching control protein/peptide||120-0P|
Amphiphysin, a dominant autoantigen in paraneoplastic Stiff-man syndrome, is a neuronal protein highly concentrated in nerve terminals. It is an abundant presynaptic protein that interacts via its COOH-terminal src homology 3 (SH3) domain with the GTPase dynamin and the inositol-5-phosphatase synaptojanin and additionally directly to clathrin.
Amphiphysin, dynamin and synaptojanin have a putative role in synaptic vesicle recycling and undergo rapid dephosphorylation in synaptosomes stimulated by a depolarizing stimulus. Two isoforms are known which seem to act in concert as a heterodimer.