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Amphiphysin - 120 002

Nerve-terminal protein binding clathrin, dynamin, and synaptojanin
Polyclonal rabbit antiserum
Cat. No.: 120 002
Amount: 200 µl
Price: $350.00
Cat. No. 120 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 400 gallery  
IHC-P/FFPE: not tested yet
Immunogen Synthetic peptide corresponding to AA 2 to 15 from rat Amphiphysin (UniProt Id: O08838)
Reactivity Reacts with: human (P49418), rat (O08838), mouse (Q7TQF7), hamster, cow, chicken.
Other species not tested yet.
Specificity Specific for amphiphysin.
Matching control protein/peptide 120-0P
Data sheet 120_002.pdf

References for Amphiphysin - 120 002

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 120 002 WB, ICC, IHC; tested species: mouse,rat
Point mutation in syntaxin-1A causes abnormal vesicle recycling, behaviors, and short term plasticity.
Watanabe Y, Katayama N, Takeuchi K, Togano T, Itoh R, Sato M, Yamazaki M, Abe M, Sato T, Oda K, Yokoyama M, et al.
The Journal of biological chemistry (2013) 28848: 34906-19. 120 002 WB; tested species: mouse
Sleep deprivation-induced protein changes in basal forebrain: implications for synaptic plasticity.
Basheer R, Brown R, Ramesh V, Begum S, McCarley RW
Journal of neuroscience research (2005) 825: 650-8. 120 002 WB
SCAMP1 function in endocytosis.
Fernández-Chacón R, Achiriloaie M, Janz R, Albanesi JP, Südhof TC
The Journal of biological chemistry (2000) 27517: 12752-6. 120 002 WB
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 120 002 WB, ICC, IHC; tested species: mouse,rat
Role of the clathrin terminal domain in regulating coated pit dynamics revealed by small molecule inhibition.
von Kleist L, Stahlschmidt W, Bulut H, Gromova K, Puchkov D, Robertson MJ, MacGregor KA, Tomilin N, Tomlin N, Pechstein A, Chau N, et al.
Cell (2011) 1463: 471-84. 120 002 ICC; tested species: mouse
A pathologic cascade leading to synaptic dysfunction in alpha-synuclein-induced neurodegeneration.
Scott DA, Tabarean I, Tang Y, Cartier A, Masliah E, Roy S
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3024: 8083-95. 120 002 ICC
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 120 002 WB, ICC, IHC; tested species: mouse,rat
Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse.
Fuchs M, Brandstätter JH, Regus-Leidig H
Frontiers in cellular neuroscience (2014) 8: 60. 120 002 IHC, EM; tested species: rat
Modes and regulation of endocytic membrane retrieval in mouse auditory hair cells.
Neef J, Jung S, Wong AB, Reuter K, Pangrsic T, Chakrabarti R, Kügler S, Lenz C, Nouvian R, Boumil RM, Frankel WN, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 343: 705-16. 120 002 IHC; tested species: mouse
Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses.
Von Kriegstein K, Schmitz F, Link E, Südhof TC
The European journal of neuroscience (1999) 114: 1335-48. 120 002 IHC
Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse.
Fuchs M, Brandstätter JH, Regus-Leidig H
Frontiers in cellular neuroscience (2014) 8: 60. 120 002 IHC, EM; tested species: rat
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 120 002
Cat. No.: 120 002
Quantity: 200 µl
Price: $350.00
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 120 002 WB, ICC, IHC; tested species: mouse,rat
Point mutation in syntaxin-1A causes abnormal vesicle recycling, behaviors, and short term plasticity.
Watanabe Y, Katayama N, Takeuchi K, Togano T, Itoh R, Sato M, Yamazaki M, Abe M, Sato T, Oda K, Yokoyama M, et al.
The Journal of biological chemistry (2013) 28848: 34906-19. 120 002 WB; tested species: mouse
Sleep deprivation-induced protein changes in basal forebrain: implications for synaptic plasticity.
Basheer R, Brown R, Ramesh V, Begum S, McCarley RW
Journal of neuroscience research (2005) 825: 650-8. 120 002 WB
SCAMP1 function in endocytosis.
Fernández-Chacón R, Achiriloaie M, Janz R, Albanesi JP, Südhof TC
The Journal of biological chemistry (2000) 27517: 12752-6. 120 002 WB
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 120 002 WB, ICC, IHC; tested species: mouse,rat
Role of the clathrin terminal domain in regulating coated pit dynamics revealed by small molecule inhibition.
von Kleist L, Stahlschmidt W, Bulut H, Gromova K, Puchkov D, Robertson MJ, MacGregor KA, Tomilin N, Tomlin N, Pechstein A, Chau N, et al.
Cell (2011) 1463: 471-84. 120 002 ICC; tested species: mouse
A pathologic cascade leading to synaptic dysfunction in alpha-synuclein-induced neurodegeneration.
Scott DA, Tabarean I, Tang Y, Cartier A, Masliah E, Roy S
The Journal of neuroscience : the official journal of the Society for Neuroscience (2010) 3024: 8083-95. 120 002 ICC
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 120 002 WB, ICC, IHC; tested species: mouse,rat
Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse.
Fuchs M, Brandstätter JH, Regus-Leidig H
Frontiers in cellular neuroscience (2014) 8: 60. 120 002 IHC, EM; tested species: rat
Modes and regulation of endocytic membrane retrieval in mouse auditory hair cells.
Neef J, Jung S, Wong AB, Reuter K, Pangrsic T, Chakrabarti R, Kügler S, Lenz C, Nouvian R, Boumil RM, Frankel WN, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2014) 343: 705-16. 120 002 IHC; tested species: mouse
Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses.
Von Kriegstein K, Schmitz F, Link E, Südhof TC
The European journal of neuroscience (1999) 114: 1335-48. 120 002 IHC
Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse.
Fuchs M, Brandstätter JH, Regus-Leidig H
Frontiers in cellular neuroscience (2014) 8: 60. 120 002 IHC, EM; tested species: rat
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 120 002
Background

Amphiphysin, a dominant autoantigen in paraneoplastic Stiff-man syndrome, is a neuronal protein highly concentrated in nerve terminals. It is an abundant presynaptic protein that interacts via its COOH-terminal src homology 3 (SH3) domain with the GTPase dynamin and the inositol-5-phosphatase synaptojanin and additionally directly to clathrin.
Amphiphysin, dynamin and synaptojanin have a putative role in synaptic vesicle recycling and undergo rapid dephosphorylation in synaptosomes stimulated by a depolarizing stimulus. Two isoforms are known which seem to act in concert as a heterodimer.