Synaptic Systems

Cat. No.: 119 011BT

Amount: 100 µg

Price: $465.00

ICC

Background

SV2s (synaptic vesicle protein 2) are integral membrane glycoproteins present in all synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2 A, SV2 B and SV2 C. SV2 A is expressed ubiquitously throughout the brain and is probably involved in the maintenance of a pool of synaptic vesicles competent for calcium- stimulated exocytosis. SV2 B has a more restricted distribution with varying degrees of coexpression with SV2 A. SV2 C is more closely related to SV2 A but shows a very restricted expression pattern. The highest expression levels were observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb. SV2 expression has also been observed in other organs. In kidney it localizes to podocytes.

1 ) Xu T et al., Nat. Cell Biol. (2001)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Fernández-Chacón R et al., Annu. Rev. Physiol. (1999)
4 ) Südhof TC et al., Nature (1995)
5 ) Bajjalieh SM et al., J. Neurosci. (1994)
6 ) Bajjalieh SM et al., Science (1992)
7 ) Feany MB et al., Cell (1992)

Protocols

WB

IP

Standard protocol

ICC

OTHER

Preabsorbtion

Other products for this target

Cat. No. 119 011BT	100 µg purified IgG, lyophilized, labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) IP: yes ICC: 1 : 100 up to 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	biotin
Clone	17G10
Subtype	IgG3
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of human SV2 A (UniProt Id: Q7L0J3)
Reactivity	Reacts with: human (Q7L0J3), rat (Q02563), mouse (Q9JIS5). Other species not tested yet.
Matching control protein/peptide	119-0P
Remarks	WB: SV2 A aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.
Data sheet	119_011bt.pdf

SV2 A antibody - 119 011BT