Tailor-made Antibodies
and Tools for Life Science

SV2 A antibody - 119 002

SV2s are highly glycosylated synaptic vesicle proteins with homology to transmembrane transporters
Rabbit polyclonal antiserum
Cat. No.: 119 002
Amount: 200 µl
Price: $365.00
Cat. No. 119 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 200 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 200 gallery  
EM: yes
Immunogen Synthetic peptide corresponding to AA 2 to 17 from human SV2A (UniProt Id: Q7L0J3)
Reactivity Reacts with: human (Q7L0J3), rat (Q02563), mouse (Q9JIS5), Guinea pig, cow, hamster, chicken, zebrafish.
Other species not tested yet.
Matching control protein/peptide 119-0P
Remarks

WB:  SV2 A aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples.

Data sheet 119_002.pdf

References for SV2 A - 119 002

Autophagy enables microglia to engage amyloid plaques and prevents microglial senescence.
Choi I, Wang M, Yoo S, Xu P, Seegobin SP, Li X, Han X, Wang Q, Peng J, Zhang B, Yue Z, et al.
Nature cell biology (2023) 257: 963-974. 119 002 WB; tested species: mouse
SV2B defines a subpopulation of synaptic vesicles.
Paulussen I, Beckert H, Musial TF, Gschossmann LJ, Wolf J, Schmitt M, Clasadonte J, Mairet-Coello G, Wolff C, Schoch S, Dietrich D, et al.
Journal of molecular cell biology (2023) : . 119 002 WB, IHC, EM; tested species: mouse
Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3.
Upmanyu N, Jin J, Emde HV, Ganzella M, Bösche L, Malviya VN, Zhuleku E, Politi AZ, Ninov M, Silbern I, Leutenegger M, et al.
Neuron (2022) : . 119 002 WB; tested species: rat
Abnormal Striatal Development Underlies the Early Onset of Behavioral Deficits in Shank3B-/- Mice.
Peixoto RT, Chantranupong L, Hakim R, Levasseur J, Wang W, Merchant T, Gorman K, Budnik B, Sabatini BL
Cell reports (2019) 297: 2016-2027.e4. 119 002 WB; tested species: mouse
The Microglial Innate Immune Receptor TREM2 Is Required for Synapse Elimination and Normal Brain Connectivity.
Filipello F, Morini R, Corradini I, Zerbi V, Canzi A, Michalski B, Erreni M, Markicevic M, Starvaggi-Cucuzza C, Otero K, Piccio L, et al.
Immunity (2018) : . 119 002 WB; tested species: mouse
Pentraxin 3 regulates synaptic function by inducing AMPA receptor clustering via ECM remodeling and β1-integrin.
Fossati G, Pozzi D, Canzi A, Mirabella F, Valentino S, Morini R, Ghirardini E, Filipello F, Moretti M, Gotti C, Annis DS, et al.
The EMBO journal (2018) : . 119 002 WB; tested species: mouse
Serotonin Transporter Associated Protein Complexes Are Enriched in Synaptic Vesicle Proteins and Proteins Involved in Energy Metabolism and Ion Homeostasis.
Haase J, Grudzinska-Goebel J, Müller HK, Münster-Wandowski A, Chow E, Wynne K, Farsi Z, Zander JF, Ahnert-Hilger G
ACS chemical neuroscience (2017) 85: 1101-1116. 119 002 WB; tested species: mouse,rat
Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
Biesemann C, Grønborg M, Luquet E, Wichert SP, Bernard V, Bungers SR, Cooper B, Varoqueaux F, Li L, Byrne JA, Urlaub H, et al.
The EMBO journal (2014) 332: 157-70. 119 002 WB; tested species: mouse
Identification of the SV2 protein receptor-binding site of botulinum neurotoxin type E.
Mahrhold S, Strotmeier J, Garcia-Rodriguez C, Lou J, Marks JD, Rummel A, Binz T
The Biochemical journal (2013) 4531: 37-47. 119 002 WB
Preferential entry of botulinum neurotoxin A Hc domain through intestinal crypt cells and targeting to cholinergic neurons of the mouse intestine.
Couesnon A, Molgó J, Connan C, Popoff MR
PLoS pathogens (2012) 83: e1002583. 119 002 WB
Cortico-hippocampal hyperexcitability in synapsin I/II/III knockout mice: age-dependency and response to the antiepileptic drug levetiracetam.
Boido D, Farisello P, Cesca F, Ferrea E, Valtorta F, Benfenati F, Baldelli P
Neuroscience (2010) 1711: 268-83. 119 002 WB
Decreased expression of synaptic vesicle protein 2A, the binding site for levetiracetam, during epileptogenesis and chronic epilepsy.
van Vliet EA, Aronica E, Redeker S, Boer K, Gorter JA
Epilepsia (2009) 503: 422-33. 119 002 WB, IHC, IHC-P
Botulinum neurotoxins C, E and F bind gangliosides via a conserved binding site prior to stimulation-dependent uptake with botulinum neurotoxin F utilising the three isoforms of SV2 as second receptor.
Rummel A, Häfner K, Mahrhold S, Darashchonak N, Holt M, Jahn R, Beermann S, Karnath T, Bigalke H, Binz T
Journal of neurochemistry (2009) 1106: 1942-54. 119 002 WB; tested species: mouse
Receptor-mediated transcytosis of botulinum neurotoxin A through intestinal cell monolayers.
Couesnon A, Pereira Y, Popoff MR
Cellular microbiology (2008) 102: 375-87. 119 002 WB
SV2C is a synaptic vesicle protein with an unusually restricted localization: anatomy of a synaptic vesicle protein family.
Janz R, Südhof TC
Neuroscience (1999) 944: 1279-90. 119 002 WB, IHC
Cat. No.: 119 002
Amount: 200 µl
Price: $365.00