Synaptic Systems - Dynamin1/2/3

Cat. No.: 115 003

Amount: 50 µg

Price: $455.00

Cat. No. 115 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 2 to 17 from rat Dynamin1 (UniProt Id: P21575)
Reactivity	Reacts with: human (Q05193, P50570, Q9UQ16), rat (P21575, P39052, Q08877), mouse (P39053, P39054, Q8BZ98), cow. Other species not tested yet.
Specificity	Recognizes dynamin 1, 2 and 3 with a preference for dynamin 1.
Matching control protein/peptide	115-0P
Data sheet	115_003.pdf

References for Dynamin1/2/3 - 115 003

Identification of Synaptic DGKθ Interactors That Stimulate DGKθ Activity.
Barber CN, Goldschmidt HL, Ma Q, Devine LR, Cole RN, Huganir RL, Raben DM
Frontiers in synaptic neuroscience (2022) 14: 855673. 115 003 WB; tested species: rat

Cat. No.: 115 003

Amount: 50 µg

Price: $455.00

ICC

IHC

IHC-P

Background

Dynamin was discovered because of its binding to microtubules. It was later shown not to function in the cytoskeleton but in endocytosis. Dynamin is required for clathrin - mediated endocytosis. It contains a NH2 - terminal GTPase domain, a middle pleckstrin - homology domain, and a COOH- terminal proline - rich sequence. The COOH - terminal sequence binds to amphiphilin which contains a SH3 domain that recognizes the proline - rich sequence of dynamin.
There are at least three isoforms of dynamin: Dynamin 1 is enriched in synapses whereas dynamin 2 is ubiquitous and dynamin 3 is expressed in brain and testis. Neuronal dynamin 1 is phosphorylated by protein kinase C and dephosphorylated by calcineurin during an action potential in the nerve terminal. It is possible that the dephosphorylation provides a trigger for endocytosis.

1 ) Hinshaw JE et al., Annu. Rev. Cell Dev. Biol. (2000)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Slepnev VI et al., Nat. Rev. Neurosci. (2000)
4 ) Brodin L et al., Curr. Opin. Neurobiol. (2000)
5 ) Hannah MJ et al., Annu. Rev. Cell Dev. Biol. (1999)
6 ) Südhof TC et al., Nature (1995)
7 ) McMahon HT et al., Cell (1995)
8 ) Sontag JM et al., J. Biol. Chem. (1994)
9 ) Robinson PJ et al., Nature (1993)
10 ) Obar RA et al., Nature (1990)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Dynamin1/2/3 antibody - 115 003

References for Dynamin1/2/3 - 115 003