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Home > Products > 114 103

GluN1 antibody - 114 103

GluN1 is the major subunit of ionotropic NMDA type glutamate receptors
Rabbit polyclonal purified antibody
Cat. No.: 114 103
Amount: 50 µg
Price: $375.00
Cat. No. 114 103 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended (see remarks)
IP: not tested yet
ICC: 1 : 250 up to 1 : 500 (see remarks) gallery  
IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 35 to 53 from rat GluN1 (UniProt Id: P35439)
Reactivity Reacts with: human (Q05586), rat (P35439), mouse (P35438).
Other species not tested yet.
Matching control protein/peptide 114-0P
Remarks

WB: Cat. no. 114 003, is recommended for this application.
ICC: Only suitable for the surface staining of living cells.
IHC: This antibody requires antigen retrieval with pepsin according to:
Lorincz A & Nusser Z (2008).
Data sheet 114_103.pdf

References for GluN1 - 114 103

Human cerebrospinal fluid monoclonal N-methyl-D-aspartate receptor autoantibodies are sufficient for encephalitis pathogenesis.
Kreye J, Wenke NK, Chayka M, Leubner J, Murugan R, Maier N, Jurek B, Ly LT, Brandl D, Rost BR, Stumpf A, et al.
Brain : a journal of neurology (2016) 139Pt 10: 2641-2652. 114 103 ICC; tested species: mouse,rat
Disruption of the grid cell network in a mouse model of early Alzheimer's disease.
Ying J, Keinath AT, Lavoie R, Vigneault E, El Mestikawy S, Brandon MP
Nature communications (2022) 131: 886. 114 103 IHC; tested species: mouse
Anti-NMDA Receptor Encephalitis in the Polar Bear (Ursus maritimus) Knut.
Prüss H, Leubner J, Wenke NK, Czirják GÁ, Szentiks CA, Greenwood AD
Scientific reports (2015) 5: 12805. 114 103 IHC
Activity-dependent regulation of MHC class I expression in the developing primary visual cortex of the common marmoset monkey.
Ribic A, Flügge G, Schlumbohm C, Mätz-Rensing K, Walter L, Fuchs E
Behavioral and brain functions : BBF (2011) 7: 1. 114 103 IHC
Cat. No.: 114 103
Amount: 50 µg
Price: $375.00
Human cerebrospinal fluid monoclonal N-methyl-D-aspartate receptor autoantibodies are sufficient for encephalitis pathogenesis.
Kreye J, Wenke NK, Chayka M, Leubner J, Murugan R, Maier N, Jurek B, Ly LT, Brandl D, Rost BR, Stumpf A, et al.
Brain : a journal of neurology (2016) 139Pt 10: 2641-2652. 114 103 ICC; tested species: mouse,rat
Disruption of the grid cell network in a mouse model of early Alzheimer's disease.
Ying J, Keinath AT, Lavoie R, Vigneault E, El Mestikawy S, Brandon MP
Nature communications (2022) 131: 886. 114 103 IHC; tested species: mouse
Anti-NMDA Receptor Encephalitis in the Polar Bear (Ursus maritimus) Knut.
Prüss H, Leubner J, Wenke NK, Czirják GÁ, Szentiks CA, Greenwood AD
Scientific reports (2015) 5: 12805. 114 103 IHC
Activity-dependent regulation of MHC class I expression in the developing primary visual cortex of the common marmoset monkey.
Ribic A, Flügge G, Schlumbohm C, Mätz-Rensing K, Walter L, Fuchs E
Behavioral and brain functions : BBF (2011) 7: 1. 114 103 IHC
ICC
ICC
IHC
Background

GluNs (NMDA-receptors) represent a class of glutamate receptors that are of central importance in synaptic plasticity. Multiple NMDA receptor subtypes exist: GluN1 and GluN2 A-D. GluN1 is the most important as it is required for activity. NMDA-receptors allow Ca2+ influx and are thought to trigger Ca2+ dependent postsynaptic processes involved in long term potentiation and depression.
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