GluN1 antibody extracellular - 114 003

GluN1 is the major subunit of ionotropic NMDA type glutamate receptors

Rabbit polyclonal purified antibody

Cat. No.: 114 003

Amount: 50 µg

Price: $375.00

Cat. No. 114 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes (see remarks) ICC: not recommended IHC: not recommended (see remarks) IHC-P: not tested yet ELISA: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Immunogen	Synthetic peptide corresponding to AA 35 to 53 from rat GluN1 (UniProt Id: P35439)
Reactivity	Reacts with: human (Q05586), rat (P35439), mouse (P35438). No signal: zebrafish. Other species not tested yet.
Matching control protein/peptide	114-0P
Remarks	IP: For most effective IP, use the denaturing IP-protocol. Consider that protein-protein interactions may be affected. IHC: Cat. no. 114 103, is recommended for IHC. ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 114 011 as capture antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet	114_003.pdf

References for GluN1 - 114 003

Molecular Characterization of AMPA-Receptor-Containing Vesicles.
Peters JJ, Leitz J, Oses-Prieto JA, Burlingame AL, Brunger AT
Frontiers in molecular neuroscience (2021) 14: 754631. 114 003 WB; tested species: mouse

Chronic Toxoplasma infection is associated with distinct alterations in the synaptic protein composition.
Lang D, Schott BH, van Ham M, Morton L, Kulikovskaja L, Herrera-Molina R, Pielot R, Klawonn F, Montag D, Jänsch L, Gundelfinger ED, et al.
Journal of neuroinflammation (2018) 151: 216. 114 003 WB; tested species: mouse

Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission.
Vasileva M, Horstmann H, Geumann C, Gitler D, Kuner T
The European journal of neuroscience (2012) 368: 3005-20. 114 003 ELISA

Cat. No.: 114 003

Amount: 50 µg

Price: $375.00

Background

GluNs (NMDA-receptors) represent a class of glutamate receptors that are of central importance in synaptic plasticity. Multiple NMDA receptor subtypes exist: GluN1 and GluN2 A-D. GluN1 is the most important as it is required for activity. NMDA-receptors allow Ca²⁺ influx and are thought to trigger Ca²⁺ dependent postsynaptic processes involved in long term potentiation and depression.

1 ) Nusser Z et al., Curr. Opin. Neurobiol. (2000)
2 ) Brose N et al., J. Biol. Chem. (1994)
3 ) Moriyoshi K et al., Nature (1991)
4 ) Collingridge GL et al., Neuropharmacology (2009)

Protocols

Standard protocol

ELISA

Membrane proteins

OTHER

Preabsorbtion

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