Synaptic Systems - Clathrin light chain

Cat. No.: 113 011

Amount: 100 µg

Price: $420.00

Cat. No. 113 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 5000 up to 1 : 10000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 gallery IHC-P: 1 : 2000 gallery
Clone	57.4
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to AA 156 to 173 from rat Clathrin light chainB (UniProt Id: P08082)
Reactivity	Reacts with: human (P09497), rat (P08082), mouse (Q6IRU5), zebrafish. Other species not tested yet.
Specificity	Specific for both neuronal light chains, does not cross-react with the non-neuronal variants.
Remarks	Suitable for immunogold electron microscopy.
Data sheet	113_011.pdf

References for Clathrin light chain - 113 011

Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson's disease.
Vidyadhara DJ, Somayaji M, Wade N, Yücel B, Zhao H, Shashaank N, Ribaudo J, Gupta J, Lam TT, Sames D, Greene LE, et al.
Cell reports (2023) 423: 112231. 113 011 WB, IHC; tested species: mouse

Vesicular Synaptobrevin/VAMP2 Levels Guarded by AP180 Control Efficient Neurotransmission.
Koo SJ, Kochlamazashvili G, Rost B, Puchkov D, Gimber N, Lehmann M, Tadeus G, Schmoranzer J, Rosenmund C, Haucke V, Maritzen T, et al.
Neuron (2015) 882: 330-44. 113 011 WB; tested species: mouse

Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting.
Biesemann C, Grønborg M, Luquet E, Wichert SP, Bernard V, Bungers SR, Cooper B, Varoqueaux F, Li L, Byrne JA, Urlaub H, et al.
The EMBO journal (2014) 332: 157-70. 113 011 WB; tested species: mouse

Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 113 011 WB; tested species: mouse

CSPα knockout causes neurodegeneration by impairing SNAP-25 function.
Sharma M, Burré J, Bronk P, Zhang Y, Xu W, Südhof TC
The EMBO journal (2012) 314: 829-41. 113 011 WB; tested species: mouse

Role for Reelin in neurotransmitter release.
Hellwig S, Hack I, Kowalski J, Brunne B, Jarowyj J, Unger A, Bock HH, Junghans D, Frotscher M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 317: 2352-60. 113 011 WB

APP/PS1KI bigenic mice develop early synaptic deficits and hippocampus atrophy.
Breyhan H, Wirths O, Duan K, Marcello A, Rettig J, Bayer TA
Acta neuropathologica (2009) 1176: 677-85. 113 011 WB; tested species: mouse

A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis.
Ferguson SM, Brasnjo G, Hayashi M, Wölfel M, Collesi C, Giovedi S, Raimondi A, Gong LW, Ariel P, Paradise S, O'toole E, et al.
Science (New York, N.Y.) (2007) 3165824: 570-4. 113 011 WB, ICC

Stimulation of phosphatidylinositol kinase type I-mediated phosphatidylinositol (4,5)-bisphosphate synthesis by AP-2mu-cargo complexes.
Krauss M, Kukhtina V, Pechstein A, Haucke V
Proceedings of the National Academy of Sciences of the United States of America (2006) 10332: 11934-9. 113 011 WB

The neuronal form of adaptor protein-3 is required for synaptic vesicle formation from endosomes.
Blumstein J, Faundez V, Nakatsu F, Saito T, Ohno H, Kelly RB
The Journal of neuroscience : the official journal of the Society for Neuroscience (2001) 2120: 8034-42. 113 011 WB

Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses.
Von Kriegstein K, Schmitz F, Link E, Südhof TC
The European journal of neuroscience (1999) 114: 1335-48. 113 011 WB, IHC

Cat. No.: 113 011

Amount: 100 µg

Price: $420.00

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IHC

IHC-P

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Clathrin light chain antibody - 113 011

References for Clathrin light chain - 113 011