Synaptic Systems - alpha/beta SNAP

Cat. No.: 112 111

Amount: 100 µg

Price: $420.00

Cat. No. 112 111	100 µg purified IgG, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery IP: yes (see remarks) ICC: 1 : 500 up to 1 : 1000 gallery IHC: yes IHC-P: not tested yet
Clone	77.2
Subtype	IgG1 (κ light chain)
Immunogen	Recombinant protein corresponding to AA 1 to 295 from rat α SNAP (UniProt Id: P54921)
Reactivity	Reacts with: human (P54920, P60880), rat (P54921, P60881), mouse (Q9DB05, P28663), zebrafish. Other species not tested yet.
Specificity	Specific for α- and β SNAP, does not cross-react to γ SNAP.
Remarks	IP: The antibody does not immunoprecipitate the 20 S SNARE-complex.
Data sheet	112_111.pdf

References for alpha/beta SNAP - 112 111

Intersectin-Mediated Clearance of SNARE Complexes Is Required for Fast Neurotransmission.
Jäpel M, Gerth F, Sakaba T, Bacetic J, Yao L, Koo SJ, Maritzen T, Freund C, Haucke V
Cell reports (2020) 302: 409-420.e6. 112 111 WB; tested species: mouse

Cortical Granule Exocytosis Is Mediated by Alpha-SNAP and N-Ethilmaleimide Sensitive Factor in Mouse Oocytes.
de Paola M, Bello OD, Michaut MA
PloS one (2015) 108: e0135679. 112 111 WB

Ubiquitin-Synaptobrevin Fusion Protein Causes Degeneration of Presynaptic Motor Terminals in Mice.
Liu Y, Li H, Sugiura Y, Han W, Gallardo G, Khvotchev M, Zhang Y, Kavalali ET, Südhof TC, Lin W
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3533: 11514-31. 112 111 WB

Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 112 111 WB, ICC, IHC; tested species: mouse,rat

An essential and NSF independent role for α-SNAP in store-operated calcium entry.
Miao Y, Miner C, Zhang L, Hanson PI, Dani A, Vig M
eLife (2013) 2: e00802. 112 111 WB; KD verified

Doc2b is a high-affinity Ca2+ sensor for spontaneous neurotransmitter release.
Groffen AJ, Martens S, Díez Arazola R, Cornelisse LN, Lozovaya N, de Jong AP, Goriounova NA, Habets RL, Takai Y, Borst JG, Brose N, et al.
Science (New York, N.Y.) (2010) 3275973: 1614-8. 112 111 WB; tested species: mouse

SNARE proteins are highly enriched in lipid rafts in PC12 cells: implications for the spatial control of exocytosis.
Chamberlain LH, Burgoyne RD, Gould GW
Proceedings of the National Academy of Sciences of the United States of America (2001) 9810: 5619-24. 112 111 WB; tested species: rat

The N-ethylmaleimide-sensitive fusion protein and alpha-SNAP induce a conformational change in syntaxin.
Hanson PI, Otto H, Barton N, Jahn R
The Journal of biological chemistry (1995) 27028: 16955-61. 112 111 WB

Cat. No.: 112 111

Amount: 100 µg

Price: $420.00

ICC

Background

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alpha/beta SNAP antibody - 112 111

References for alpha/beta SNAP - 112 111