|Cat. No. 112 011||100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery
IP: yes (see remarks)
ICC: not recommended (denaturation of the antigen during fixation)
IHC: not tested yet
IHC-P/FFPE: not tested yet
|Subtype||IgG2b (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 1 to 295 from rat α SNAP (UniProt Id: P54921)|
Reacts with: human (P54920, P60880), rat (P54921, P60881), mouse (Q9DB05, P28663), zebrafish.
Other species not tested yet.
|Specificity||Specific for α- and β SNAP, does not cross-react to γ SNAP.|
IP: The antibody does not immunoprecipitate the 20 S SNARE-complex.
The proteins α/β-SNAP are two related soluble and highly conserved proteins that bind to the fusion complex (SNARE complex), thus allowing the N-ethylmaleimide sensitive fusion protein NSF to bind to the complex. γ-SNAP binds directly to NSF and Gaf-1/Rip11, a protein of the Rab11 interacting family. In contrast to α/β-SNAP it does not interact directly with SNARE proteins and is not required for ER-Golgi transport. SNAP-proteins are abundantly expressed in all tissues. They are partially soluble, partially membrane-bound.