Cat. No.: 111 318
Amount: 50 µg
Price:
$415.00
|
|
|
| Cat. No. 111 318 |
50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Clone | Gp71.2 |
| Subtype | IgG2 (κ light chain) |
| Immunogen | Full-length recombinant rat SNAP25B protein (UniProt Id: P60881-1) |
| Epitop |
AA 1 to 20 from rat SNAP25B (UniProt Id: P60881-1) |
| Reactivity |
Reacts with: human (P60880), rat (P60881), mouse (P60879), mammals, bovine. No signal: zebrafish. Other species not tested yet. |
| Specificity | Detects both splice variants SNAP 25A and B. Recognizes the Botulinum neurotoxin A and E cleavage products. |
| Remarks |
This antibody is a chimeric antibody based on the monoclonal mouse antibody 71.2. The constant regions of the heavy and light chains have been replaced with guinea pig specific sequences. Therefore, the antibody can be used with standard anti-guinea pig secondary reagents. The antibody has been expressed in mammalian cells. |
| Data sheet | 111_318.pdf |
|
|
SNAP25 (synaptosome-associated protein of 25 kD) is a highly conserved protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP25 is the target of Botulinum neurotoxins A and E which cleave off 9 and 26 amino acids, respectively, from the C-terminus.
SNAP25 is part of the exocytotic fusion complex (v-SNARE) of neurons where it assembles with syntaxin1 and synaptobrevin. It is abundantly localized on the neuronal plasmalemma and on recycling vesicles including synaptic vesicles. It is also expressed in neuroendocrine cells. There are two splice-variants, SNAP25A and 25B.