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SNAP29 antibody - 111 302 K.O.

SNAP29 is a Relative of SNAP25 that distributes between the cytoplasma and organellar membranes
Rabbit polyclonal antiserum
Cat. No.: 111 302
Amount: 200 µl
Price: $355.00
Cat. No. 111 302 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: not tested yet (see remarks)
IHC: not recommended
IHC-P: not tested yet
Immunogen Recombinant protein corresponding to AA 1 to 257 from rat SNAP29 (UniProt Id: Q9Z2P6)
Reactivity Reacts with: human (O95721), rat (Q9Z2P6), mouse (Q9ERB0), hamster.
Other species not tested yet.
Specificity K.O. validated
Remarks

ICC: The affinity purified antibody (cat. no. 111 303) is highly recommended.
 

Data sheet 111_302.pdf

References for SNAP29 - 111 302

SNARE-binding protein synaptosomal-associated protein of 29 kDa (SNAP29) regulates the intracellular sequestration of glucose transporter 4 (GLUT4) vesicles in adipocytes.
Matsui K, Emoto M, Fukuda N, Nomiyama R, Yamada K, Tanizawa Y
Journal of diabetes investigation (2022) : . 111 302 WB, IP, ICC; tested species: mouse
Chromosome 22q11.2 deletion causes PERK-dependent vulnerability in dopaminergic neurons.
Arioka Y, Shishido E, Kushima I, Suzuki T, Saito R, Aiba A, Mori D, Ozaki N
EBioMedicine (2020) 63: 103138. 111 302 WB; tested species: mouse
Role of VAMP7-Dependent Secretion of Reticulon 3 in Neurite Growth.
Wojnacki J, Nola S, Bun P, Cholley B, Filippini F, Pressé MT, Lipecka J, Man Lam S, N'guyen J, Simon A, Ouslimani A, et al.
Cell reports (2020) 3312: 108536. 111 302 WB, ICC; tested species: rat
Autophagosomal YKT6 is required for fusion with lysosomes independently of syntaxin 17.
Matsui T, Jiang P, Nakano S, Sakamaki Y, Yamamoto H, Mizushima N
The Journal of cell biology (2018) 2178: 2633-2645. 111 302 WB; KD verified; tested species: human
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 111 302 WB; tested species: rat
Pancreatic acinar cells express vesicle-associated membrane protein 2- and 8-specific populations of zymogen granules with distinct and overlapping roles in secretion.
Weng N, Thomas DD, Groblewski GE
The Journal of biological chemistry (2007) 28213: 9635-45. 111 302 WB, IP
Phosphorylation of SNAP-23 in activated human platelets.
Polgár J, Lane WS, Chung SH, Houng AK, Reed GL
The Journal of biological chemistry (2003) 27845: 44369-76. 111 302 WB; tested species: human
SNARE-binding protein synaptosomal-associated protein of 29 kDa (SNAP29) regulates the intracellular sequestration of glucose transporter 4 (GLUT4) vesicles in adipocytes.
Matsui K, Emoto M, Fukuda N, Nomiyama R, Yamada K, Tanizawa Y
Journal of diabetes investigation (2022) : . 111 302 WB, IP, ICC; tested species: mouse
Pancreatic acinar cells express vesicle-associated membrane protein 2- and 8-specific populations of zymogen granules with distinct and overlapping roles in secretion.
Weng N, Thomas DD, Groblewski GE
The Journal of biological chemistry (2007) 28213: 9635-45. 111 302 WB, IP
SNARE-binding protein synaptosomal-associated protein of 29 kDa (SNAP29) regulates the intracellular sequestration of glucose transporter 4 (GLUT4) vesicles in adipocytes.
Matsui K, Emoto M, Fukuda N, Nomiyama R, Yamada K, Tanizawa Y
Journal of diabetes investigation (2022) : . 111 302 WB, IP, ICC; tested species: mouse
Role of VAMP7-Dependent Secretion of Reticulon 3 in Neurite Growth.
Wojnacki J, Nola S, Bun P, Cholley B, Filippini F, Pressé MT, Lipecka J, Man Lam S, N'guyen J, Simon A, Ouslimani A, et al.
Cell reports (2020) 3312: 108536. 111 302 WB, ICC; tested species: rat
Cat. No.: 111 302
Amount: 200 µl
Price: $355.00
SNARE-binding protein synaptosomal-associated protein of 29 kDa (SNAP29) regulates the intracellular sequestration of glucose transporter 4 (GLUT4) vesicles in adipocytes.
Matsui K, Emoto M, Fukuda N, Nomiyama R, Yamada K, Tanizawa Y
Journal of diabetes investigation (2022) : . 111 302 WB, IP, ICC; tested species: mouse
Chromosome 22q11.2 deletion causes PERK-dependent vulnerability in dopaminergic neurons.
Arioka Y, Shishido E, Kushima I, Suzuki T, Saito R, Aiba A, Mori D, Ozaki N
EBioMedicine (2020) 63: 103138. 111 302 WB; tested species: mouse
Role of VAMP7-Dependent Secretion of Reticulon 3 in Neurite Growth.
Wojnacki J, Nola S, Bun P, Cholley B, Filippini F, Pressé MT, Lipecka J, Man Lam S, N'guyen J, Simon A, Ouslimani A, et al.
Cell reports (2020) 3312: 108536. 111 302 WB, ICC; tested species: rat
Autophagosomal YKT6 is required for fusion with lysosomes independently of syntaxin 17.
Matsui T, Jiang P, Nakano S, Sakamaki Y, Yamamoto H, Mizushima N
The Journal of cell biology (2018) 2178: 2633-2645. 111 302 WB; KD verified; tested species: human
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 111 302 WB; tested species: rat
Pancreatic acinar cells express vesicle-associated membrane protein 2- and 8-specific populations of zymogen granules with distinct and overlapping roles in secretion.
Weng N, Thomas DD, Groblewski GE
The Journal of biological chemistry (2007) 28213: 9635-45. 111 302 WB, IP
Phosphorylation of SNAP-23 in activated human platelets.
Polgár J, Lane WS, Chung SH, Houng AK, Reed GL
The Journal of biological chemistry (2003) 27845: 44369-76. 111 302 WB; tested species: human
SNARE-binding protein synaptosomal-associated protein of 29 kDa (SNAP29) regulates the intracellular sequestration of glucose transporter 4 (GLUT4) vesicles in adipocytes.
Matsui K, Emoto M, Fukuda N, Nomiyama R, Yamada K, Tanizawa Y
Journal of diabetes investigation (2022) : . 111 302 WB, IP, ICC; tested species: mouse
Pancreatic acinar cells express vesicle-associated membrane protein 2- and 8-specific populations of zymogen granules with distinct and overlapping roles in secretion.
Weng N, Thomas DD, Groblewski GE
The Journal of biological chemistry (2007) 28213: 9635-45. 111 302 WB, IP
SNARE-binding protein synaptosomal-associated protein of 29 kDa (SNAP29) regulates the intracellular sequestration of glucose transporter 4 (GLUT4) vesicles in adipocytes.
Matsui K, Emoto M, Fukuda N, Nomiyama R, Yamada K, Tanizawa Y
Journal of diabetes investigation (2022) : . 111 302 WB, IP, ICC; tested species: mouse
Role of VAMP7-Dependent Secretion of Reticulon 3 in Neurite Growth.
Wojnacki J, Nola S, Bun P, Cholley B, Filippini F, Pressé MT, Lipecka J, Man Lam S, N'guyen J, Simon A, Ouslimani A, et al.
Cell reports (2020) 3312: 108536. 111 302 WB, ICC; tested species: rat
Background

SNAP 29, also known as GS 32, is an ubiquitously distributed relative of SNAP 25 and SNAP 23 that is ubiquitously distributed among intracellular membranes and that is also found in the cytosol of mammalian cells. As a Q-SNARE it forms SNARE complexes in vitro but its precise role in intracellular membrane traffic is not known.