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Home > Products > 111 203

SNAP23 antibody - 111 203 K.O.

SNAP23 is a palmitoylated plasma membrane protein; ubiquitously expressed isoform of SNAP25
Rabbit polyclonal purified antibody
Cat. No.: 111 203
Amount: 50 µg
Price: $455.00
Cat. No. 111 203 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes (see remarks)
ICC: 1 : 500
IHC: yes
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 196 to 211 from human SNAP23 (UniProt Id: O00161)
Reactivity Reacts with: human (O00161), rat (O70377), mouse (O09044), hamster, pig, rabbit.
Other species not tested yet.
Specificity K.O. validated PubMed: 32051343
Matching control protein/peptide 111-2P
Remarks

IP: Cat. no. 111 213 is recommended
Data sheet 111_203.pdf

References for SNAP23 - 111 203

The endothelial diapedesis synapse regulates transcellular migration of human T lymphocytes in a CX3CL1- and SNAP23-dependent manner.
Schoppmeyer R, van Steen ACI, Kempers L, Timmerman AL, Nolte MA, Hombrink P, van Buul JD
Cell reports (2022) 383: 110243. 111 203 WB; tested species: human
SNAP23 depletion enables more SNAP25/calcium channel excitosome formation to increase insulin exocytosis in type 2 diabetes.
Liang T, Qin T, Kang F, Kang Y, Xie L, Zhu D, Dolai S, Greitzer-Antes D, Baker RK, Feng D, Tuduri E, et al.
JCI insight (2020) 53: . 111 203 WB, IP, IHC; KO,KD verified; tested species: human,mouse
SNAP23 is required for constitutive and regulated exocytosis in mouse oocytes.
Mehlmann LM, Uliasz TF, Lowther KM
Biology of reproduction (2019) : . 111 203 WB; KD verified; tested species: mouse
Role of SNAREs in the Atopic Dermatitis-related Cytokine Secretion and Skin-Nerve Communication.
Meng J, Wang J, Buddenkotte J, Buhl T, Steinhoff M
The Journal of investigative dermatology (2019) : . 111 203 WB; KD verified; tested species: human
Phosphorylation of syntaxin-3 at Thr 14 negatively regulates exocytosis in RBL-2H3 mast cells.
Tadokoro S, Shibata T, Inoh Y, Amano T, Nakanishi M, Hirashima N, Utsunomiya-Tate N
Cell biology international (2016) 405: 589-96. 111 203 WB; tested species: rat
Syntaxin 8 regulates platelet dense granule secretion, aggregation, and thrombus stability.
Golebiewska EM, Harper MT, Williams CM, Savage JS, Goggs R, Fischer von Mollard G, Poole AW
The Journal of biological chemistry (2015) 2903: 1536-45. 111 203 WB
Renal epithelial cells can release ATP by vesicular fusion.
Bjaelde RG, Arnadottir SS, Overgaard MT, Leipziger J, Praetorius HA
Frontiers in physiology (2013) 4: 238. 111 203 WB; KD verified; tested species: rabbit
REST/NRSF governs the expression of dense-core vesicle gliosecretion in astrocytes.
Prada I, Marchaland J, Podini P, Magrassi L, D'Alessandro R, Bezzi P, Meldolesi J
The Journal of cell biology (2011) 1933: 537-49. 111 203 WB
A role for VAMP8/endobrevin in surface deployment of the water channel aquaporin 2.
Wang CC, Ng CP, Shi H, Liew HC, Guo K, Zeng Q, Hong W
Molecular and cellular biology (2010) 301: 333-43. 111 203 WB
Syntaxin 1A has a specific binding site in the H3 domain that is critical for targeting of H+-ATPase to apical membrane of renal epithelial cells.
Li G, Yang Q, Alexander EA, Schwartz JH
American journal of physiology. Cell physiology (2005) 2893: C665-72. 111 203 WB
Identification of soluble N-ethylmaleimide-sensitive factor attachment protein receptor exocytotic machinery in human plasma cells: SNAP-23 is essential for antibody secretion.
Reales E, Mora-López F, Rivas V, García-Poley A, Brieva JA, Campos-Caro A
Journal of immunology (Baltimore, Md. : 1950) (2005) 17510: 6686-93. 111 203 WB, ICC
Cholesterol-dependent syntaxin-4 and SNAP-23 clustering regulates caveolar fusion with the endothelial plasma membrane.
Predescu SA, Predescu DN, Shimizu K, Klein IK, Malik AB
The Journal of biological chemistry (2005) 28044: 37130-8. 111 203 WB, ICC
Vesicular glutamate transporter-dependent glutamate release from astrocytes.
Montana V, Ni Y, Sunjara V, Hua X, Parpura V
The Journal of neuroscience : the official journal of the Society for Neuroscience (2004) 2411: 2633-42. 111 203 WB
Intersectin regulates fission and internalization of caveolae in endothelial cells.
Predescu SA, Predescu DN, Timblin BK, Stan RV, Malik AB
Molecular biology of the cell (2003) 1412: 4997-5010. 111 203 WB
Syntaxin isoform specificity in the regulation of renal H+-ATPase exocytosis.
Li G, Alexander EA, Schwartz JH
The Journal of biological chemistry (2003) 27822: 19791-7. 111 203 WB
Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses.
Von Kriegstein K, Schmitz F, Link E, Südhof TC
The European journal of neuroscience (1999) 114: 1335-48. 111 203 WB, ICC
EHSH1/intersectin, a protein that contains EH and SH3 domains and binds to dynamin and SNAP-25. A protein connection between exocytosis and endocytosis?
Okamoto M, Schoch S, Südhof TC
The Journal of biological chemistry (1999) 27426: 18446-54. 111 203 WB
SNAP23 depletion enables more SNAP25/calcium channel excitosome formation to increase insulin exocytosis in type 2 diabetes.
Liang T, Qin T, Kang F, Kang Y, Xie L, Zhu D, Dolai S, Greitzer-Antes D, Baker RK, Feng D, Tuduri E, et al.
JCI insight (2020) 53: . 111 203 WB, IP, IHC; KO,KD verified; tested species: human,mouse
Insulin stimulates syntaxin4 SNARE complex assembly via a novel regulatory mechanism.
Kioumourtzoglou D, Gould GW, Bryant NJ
Molecular and cellular biology (2014) 347: 1271-9. 111 203 ICC
PKA-dependent ENaC trafficking requires the SNARE-binding protein complexin.
Butterworth MB, Frizzell RA, Johnson JP, Peters KW, Edinger RS
American journal of physiology. Renal physiology (2005) 2895: F969-77. 111 203 ICC
Cholesterol-dependent syntaxin-4 and SNAP-23 clustering regulates caveolar fusion with the endothelial plasma membrane.
Predescu SA, Predescu DN, Shimizu K, Klein IK, Malik AB
The Journal of biological chemistry (2005) 28044: 37130-8. 111 203 WB, ICC
Identification of soluble N-ethylmaleimide-sensitive factor attachment protein receptor exocytotic machinery in human plasma cells: SNAP-23 is essential for antibody secretion.
Reales E, Mora-López F, Rivas V, García-Poley A, Brieva JA, Campos-Caro A
Journal of immunology (Baltimore, Md. : 1950) (2005) 17510: 6686-93. 111 203 WB, ICC
Promiscuous interaction of SNAP-25 with all plasma membrane syntaxins in a neuroendocrine cell.
Bajohrs M, Darios F, Peak-Chew SY, Davletov B
The Biochemical journal (2005) 392Pt 2: 283-9. 111 203 ICC
Vimentin filaments in fibroblasts are a reservoir for SNAP23, a component of the membrane fusion machinery.
Faigle W, Colucci-Guyon E, Louvard D, Amigorena S, Galli T
Molecular biology of the cell (2000) 1110: 3485-94. 111 203 ICC
Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses.
Von Kriegstein K, Schmitz F, Link E, Südhof TC
The European journal of neuroscience (1999) 114: 1335-48. 111 203 WB, ICC
SNAP23 depletion enables more SNAP25/calcium channel excitosome formation to increase insulin exocytosis in type 2 diabetes.
Liang T, Qin T, Kang F, Kang Y, Xie L, Zhu D, Dolai S, Greitzer-Antes D, Baker RK, Feng D, Tuduri E, et al.
JCI insight (2020) 53: . 111 203 WB, IP, IHC; KO,KD verified; tested species: human,mouse
SNARE-dependent glutamate release in megakaryocytes.
Thompson CJ, Schilling T, Howard MR, Genever PG
Experimental hematology (2010) 386: 504-15. 111 203 IHC
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 111 203 DNA-PAINT; tested species: rat
Fusion-related release of glutamate from astrocytes.
Zhang Q, Pangrsic T, Kreft M, Krzan M, Li N, Sul JY, Halassa M, Van Bockstaele E, Zorec R, Haydon PG
The Journal of biological chemistry (2004) 27913: 12724-33. 111 203
Subcellular distribution of 3 functional platelet SNARE proteins: human cellubrevin, SNAP-23, and syntaxin 2.
Feng D, Crane K, Rozenvayn N, Dvorak AM, Flaumenhaft R
Blood (2002) 9911: 4006-14. 111 203
Cat. No.: 111 203
Amount: 50 µg
Price: $455.00
The endothelial diapedesis synapse regulates transcellular migration of human T lymphocytes in a CX3CL1- and SNAP23-dependent manner.
Schoppmeyer R, van Steen ACI, Kempers L, Timmerman AL, Nolte MA, Hombrink P, van Buul JD
Cell reports (2022) 383: 110243. 111 203 WB; tested species: human
SNAP23 depletion enables more SNAP25/calcium channel excitosome formation to increase insulin exocytosis in type 2 diabetes.
Liang T, Qin T, Kang F, Kang Y, Xie L, Zhu D, Dolai S, Greitzer-Antes D, Baker RK, Feng D, Tuduri E, et al.
JCI insight (2020) 53: . 111 203 WB, IP, IHC; KO,KD verified; tested species: human,mouse
SNAP23 is required for constitutive and regulated exocytosis in mouse oocytes.
Mehlmann LM, Uliasz TF, Lowther KM
Biology of reproduction (2019) : . 111 203 WB; KD verified; tested species: mouse
Role of SNAREs in the Atopic Dermatitis-related Cytokine Secretion and Skin-Nerve Communication.
Meng J, Wang J, Buddenkotte J, Buhl T, Steinhoff M
The Journal of investigative dermatology (2019) : . 111 203 WB; KD verified; tested species: human
Phosphorylation of syntaxin-3 at Thr 14 negatively regulates exocytosis in RBL-2H3 mast cells.
Tadokoro S, Shibata T, Inoh Y, Amano T, Nakanishi M, Hirashima N, Utsunomiya-Tate N
Cell biology international (2016) 405: 589-96. 111 203 WB; tested species: rat
Syntaxin 8 regulates platelet dense granule secretion, aggregation, and thrombus stability.
Golebiewska EM, Harper MT, Williams CM, Savage JS, Goggs R, Fischer von Mollard G, Poole AW
The Journal of biological chemistry (2015) 2903: 1536-45. 111 203 WB
Renal epithelial cells can release ATP by vesicular fusion.
Bjaelde RG, Arnadottir SS, Overgaard MT, Leipziger J, Praetorius HA
Frontiers in physiology (2013) 4: 238. 111 203 WB; KD verified; tested species: rabbit
REST/NRSF governs the expression of dense-core vesicle gliosecretion in astrocytes.
Prada I, Marchaland J, Podini P, Magrassi L, D'Alessandro R, Bezzi P, Meldolesi J
The Journal of cell biology (2011) 1933: 537-49. 111 203 WB
A role for VAMP8/endobrevin in surface deployment of the water channel aquaporin 2.
Wang CC, Ng CP, Shi H, Liew HC, Guo K, Zeng Q, Hong W
Molecular and cellular biology (2010) 301: 333-43. 111 203 WB
Syntaxin 1A has a specific binding site in the H3 domain that is critical for targeting of H+-ATPase to apical membrane of renal epithelial cells.
Li G, Yang Q, Alexander EA, Schwartz JH
American journal of physiology. Cell physiology (2005) 2893: C665-72. 111 203 WB
Identification of soluble N-ethylmaleimide-sensitive factor attachment protein receptor exocytotic machinery in human plasma cells: SNAP-23 is essential for antibody secretion.
Reales E, Mora-López F, Rivas V, García-Poley A, Brieva JA, Campos-Caro A
Journal of immunology (Baltimore, Md. : 1950) (2005) 17510: 6686-93. 111 203 WB, ICC
Cholesterol-dependent syntaxin-4 and SNAP-23 clustering regulates caveolar fusion with the endothelial plasma membrane.
Predescu SA, Predescu DN, Shimizu K, Klein IK, Malik AB
The Journal of biological chemistry (2005) 28044: 37130-8. 111 203 WB, ICC
Vesicular glutamate transporter-dependent glutamate release from astrocytes.
Montana V, Ni Y, Sunjara V, Hua X, Parpura V
The Journal of neuroscience : the official journal of the Society for Neuroscience (2004) 2411: 2633-42. 111 203 WB
Intersectin regulates fission and internalization of caveolae in endothelial cells.
Predescu SA, Predescu DN, Timblin BK, Stan RV, Malik AB
Molecular biology of the cell (2003) 1412: 4997-5010. 111 203 WB
Syntaxin isoform specificity in the regulation of renal H+-ATPase exocytosis.
Li G, Alexander EA, Schwartz JH
The Journal of biological chemistry (2003) 27822: 19791-7. 111 203 WB
Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses.
Von Kriegstein K, Schmitz F, Link E, Südhof TC
The European journal of neuroscience (1999) 114: 1335-48. 111 203 WB, ICC
EHSH1/intersectin, a protein that contains EH and SH3 domains and binds to dynamin and SNAP-25. A protein connection between exocytosis and endocytosis?
Okamoto M, Schoch S, Südhof TC
The Journal of biological chemistry (1999) 27426: 18446-54. 111 203 WB
SNAP23 depletion enables more SNAP25/calcium channel excitosome formation to increase insulin exocytosis in type 2 diabetes.
Liang T, Qin T, Kang F, Kang Y, Xie L, Zhu D, Dolai S, Greitzer-Antes D, Baker RK, Feng D, Tuduri E, et al.
JCI insight (2020) 53: . 111 203 WB, IP, IHC; KO,KD verified; tested species: human,mouse
Insulin stimulates syntaxin4 SNARE complex assembly via a novel regulatory mechanism.
Kioumourtzoglou D, Gould GW, Bryant NJ
Molecular and cellular biology (2014) 347: 1271-9. 111 203 ICC
PKA-dependent ENaC trafficking requires the SNARE-binding protein complexin.
Butterworth MB, Frizzell RA, Johnson JP, Peters KW, Edinger RS
American journal of physiology. Renal physiology (2005) 2895: F969-77. 111 203 ICC
Cholesterol-dependent syntaxin-4 and SNAP-23 clustering regulates caveolar fusion with the endothelial plasma membrane.
Predescu SA, Predescu DN, Shimizu K, Klein IK, Malik AB
The Journal of biological chemistry (2005) 28044: 37130-8. 111 203 WB, ICC
Identification of soluble N-ethylmaleimide-sensitive factor attachment protein receptor exocytotic machinery in human plasma cells: SNAP-23 is essential for antibody secretion.
Reales E, Mora-López F, Rivas V, García-Poley A, Brieva JA, Campos-Caro A
Journal of immunology (Baltimore, Md. : 1950) (2005) 17510: 6686-93. 111 203 WB, ICC
Promiscuous interaction of SNAP-25 with all plasma membrane syntaxins in a neuroendocrine cell.
Bajohrs M, Darios F, Peak-Chew SY, Davletov B
The Biochemical journal (2005) 392Pt 2: 283-9. 111 203 ICC
Vimentin filaments in fibroblasts are a reservoir for SNAP23, a component of the membrane fusion machinery.
Faigle W, Colucci-Guyon E, Louvard D, Amigorena S, Galli T
Molecular biology of the cell (2000) 1110: 3485-94. 111 203 ICC
Distribution of synaptic vesicle proteins in the mammalian retina identifies obligatory and facultative components of ribbon synapses.
Von Kriegstein K, Schmitz F, Link E, Südhof TC
The European journal of neuroscience (1999) 114: 1335-48. 111 203 WB, ICC
SNAP23 depletion enables more SNAP25/calcium channel excitosome formation to increase insulin exocytosis in type 2 diabetes.
Liang T, Qin T, Kang F, Kang Y, Xie L, Zhu D, Dolai S, Greitzer-Antes D, Baker RK, Feng D, Tuduri E, et al.
JCI insight (2020) 53: . 111 203 WB, IP, IHC; KO,KD verified; tested species: human,mouse
SNARE-dependent glutamate release in megakaryocytes.
Thompson CJ, Schilling T, Howard MR, Genever PG
Experimental hematology (2010) 386: 504-15. 111 203 IHC
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 111 203 DNA-PAINT; tested species: rat
Fusion-related release of glutamate from astrocytes.
Zhang Q, Pangrsic T, Kreft M, Krzan M, Li N, Sul JY, Halassa M, Van Bockstaele E, Zorec R, Haydon PG
The Journal of biological chemistry (2004) 27913: 12724-33. 111 203
Subcellular distribution of 3 functional platelet SNARE proteins: human cellubrevin, SNAP-23, and syntaxin 2.
Feng D, Crane K, Rozenvayn N, Dvorak AM, Flaumenhaft R
Blood (2002) 9911: 4006-14. 111 203
WB
Background

SNAP23 (synaptosome-associated protein of 23 kDa) is an ubiquitously expressed isoform and functional homologue of SNAP25. It is resistant to cleavage by BoNT/A and E. The protein is part of the exocytotic fusion complex (v-SNARE) where it assembles with syntaxin1 and synaptobrevin.
SNAP23 is able to function in regulated exocytosis. Both isoforms may have their own specific binding partners and discrete, albeit mechanistically similar, functional roles within the cell.
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