SNAP25 antibody - 111 111BT

SNAP25 is a palmitoylated plasma membrane protein; required for exocytosis

Mouse monoclonal purified IgG

Cat. No.: 111 111BT

Amount: 50 µg

Price: $470.00

Cat. No. 111 111BT	50 µg purified IgG, lyophilized, labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 10000 (AP staining) gallery IP: yes (see remarks) ICC: 1 : 100 up to 1 : 500 IHC: 1 : 200 IHC-P: not tested yet ELISA: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Label	biotin
Clone	71.2
Subtype	IgG1 (κ light chain)
Immunogen	Full-length recombinant rat SNAP25B protein (UniProt Id: P60881-1)
Epitop	AA 1 to 20 from rat SNAP25B (UniProt Id: P60881-1)
Reactivity	Reacts with: human (P60880), rat (P60881), mouse (P60879), mammals. No signal: zebrafish. Other species not tested yet.
Specificity	Detects both splice variants SNAP 25A and B. Recognizes the Botulinum neurotoxin A and E cleavage products.
Remarks	IP: Immunoprecipitation not quantitative, appears to depend on the binding status of the protein.
Data sheet	111_111bt.pdf

Cat. No.: 111 111BT

Amount: 50 µg

Price: $470.00

Background

SNAP25 (synaptosome-associated protein of 25 kD) is a highly conserved protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP25 is the target of Botulinum neurotoxins A and E which cleave off 9 and 26 amino acids, respectively, from the C-terminus.
SNAP25 is part of the exocytotic fusion complex (v-SNARE) of neurons where it assembles with syntaxin1 and synaptobrevin. It is abundantly localized on the neuronal plasmalemma and on recycling vesicles including synaptic vesicles. It is also expressed in neuroendocrine cells. There are two splice-variants, SNAP25A and 25B.

1 ) Lin RC et al., Annu. Rev. Cell Dev. Biol. (2000)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Jahn R et al., Annu. Rev. Biochem. (1999)
4 ) Fasshauer D et al., J. Biol. Chem. (1997)
5 ) Südhof TC et al., Nature (1995)
6 ) Blasi J et al., Nature (1993)
7 ) Prescott GR et al., BMC Neurosci (2011)
8 ) Schulze KL et al., Cell (1995)

Protocols

Standard protocol

ICC

IHC

ELISA

Standard protocol

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