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SNAP25 antibody - 111 111

SNAP25 is a palmitoylated plasma membrane protein; required for exocytosis
Mouse monoclonal purified IgG
Cat. No.: 111 111
Amount: 50 µg
Price: $430.00
Cat. No. 111 111 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 10000 (AP staining) gallery  
IP: yes (see remarks)
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: 1 : 200 gallery  
IHC-P: 1 : 1000 gallery  
ELISA: yes (see remarks)
Clone 71.2
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 206 from rat SNAP25B (UniProt Id: P60881-1)
Epitop Epitop: AA 1 to 20 from rat SNAP25B (UniProt Id: P60881-1)
Reactivity Reacts with: human (P60880), rat (P60881), mouse (P60879), mammals, bovine.
No signal: zebrafish.
Other species not tested yet.
Specificity Detects both splice variants SNAP 25A and B. Recognizes the Botulinum neurotoxin A and E cleavage products.
Remarks

IP: Immunoprecipitation not quantitative, appears to depend on the binding status of the protein.
ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 111 002 as detector antibody. The ELISA-protocol for membrane proteins is recommended.
Data sheet 111_111.pdf

References for SNAP25 - 111 111

Proximity labelling reveals effects of disease-causing mutation on the DNAJC5/cysteine string protein α interactome.
Barker E, Milburn A, Helassa N, Hammond D, Sanchez-Soriano N, Morgan A, Barclay J
The Biochemical journal (2024) : . 111 111 WB; tested species: rat
LRRTMs Organize Synapses through Differential Engagement of Neurexin and PTPσ.
Roppongi RT, Dhume SH, Padmanabhan N, Silwal P, Zahra N, Karimi B, Bomkamp C, Patil CS, Champagne-Jorgensen K, Twilley RE, Zhang P, et al.
Neuron (2020) : . 111 111 WB; tested species: mouse
VAMP4 maintains a Ca2+-sensitive pool of spontaneously recycling synaptic vesicles.
Lin PY, Chanaday NL, Horvath PM, Ramirez DMO, Monteggia LM, Kavalali ET
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 111 111 WB; tested species: rat
Potential Protection Against Type 2 Diabetes in Obesity Through Lower CD36 Expression and Improved Exocytosis in β-Cells.
Nagao M, Esguerra JLS, Asai A, Ofori JK, Edlund A, Wendt A, Sugihara H, Wollheim CB, Oikawa S, Eliasson L
Diabetes (2020) 696: 1193-1205. 111 111 WB; tested species: human
Role of Aberrant Spontaneous Neurotransmission in SNAP25-Associated Encephalopathies.
Alten B, Zhou Q, Shin OH, Esquivies L, Lin PY, White KI, Sun R, Chung WK, Monteggia LM, Brunger AT, Kavalali ET, et al.
Neuron (2020) : . 111 111 WB; tested species: mouse
Intracellular Vesicle Fusion Requires a Membrane-Destabilizing Peptide Located at the Juxtamembrane Region of the v-SNARE.
Rathore SS, Liu Y, Yu H, Wan C, Lee M, Yin Q, Stowell MHB, Shen J
Cell reports (2019) 2913: 4583-4592.e3. 111 111 WB
Phosphorylated SNAP25 in the CA1 regulates morphine-associated contextual memory retrieval via increasing GluN2B-NMDAR surface localization.
Wang X, Liu Y, Jia M, Sun X, Wang N, Li Y, Cui C
Addiction biology (2017) : . 111 111 WB; tested species: rat
Identification and characterization of a novel botulinum neurotoxin.
Zhang S, Masuyer G, Zhang J, Shen Y, Lundin D, Henriksson L, Miyashita SI, Martínez-Carranza M, Dong M, Stenmark P
Nature communications (2017) 8: 14130. 111 111 WB; tested species: mouse
The neural cell adhesion molecule promotes maturation of the presynaptic endocytotic machinery by switching synaptic vesicle recycling from adaptor protein 3 (AP-3)- to AP-2-dependent mechanisms.
Shetty A, Sytnyk V, Leshchyns'ka I, Puchkov D, Haucke V, Schachner M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3342: 16828-45. 111 111 WB
Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 111 111 WB; tested species: mouse
Aberrant function and structure of retinal ribbon synapses in the absence of complexin 3 and complexin 4.
Reim K, Regus-Leidig H, Ammermüller J, El-Kordi A, Radyushkin K, Ehrenreich H, Brandstätter JH, Brose N
Journal of cell science (2009) 122Pt 9: 1352-61. 111 111 WB, IHC; tested species: mouse
Dvl regulates endo- and exocytotic processes through binding to synaptotagmin.
Kishida S, Hamao K, Inoue M, Hasegawa M, Matsuura Y, Mikoshiba K, Fukuda M, Kikuchi A
Genes to cells : devoted to molecular & cellular mechanisms (2007) 121: 49-61. 111 111 WB; tested species: rat
Loss of SNAP-25 and rabphilin 3a in sensory-motor cortex in Huntington's disease.
Smith R, Klein P, Koc-Schmitz Y, Waldvogel HJ, Faull RL, Brundin P, Plomann M, Li JY
Journal of neurochemistry (2007) 1031: 115-23. 111 111 WB; tested species: human
Rapid and Sensitive Nano-Immunosensors for Botulinum.
Cheng HP, Chuang HS
ACS sensors (2019) : . 111 111 IP; tested species: human
The N-ethylmaleimide-sensitive fusion protein and alpha-SNAP induce a conformational change in syntaxin.
Hanson PI, Otto H, Barton N, Jahn R
The Journal of biological chemistry (1995) 27028: 16955-61. 111 111 IP
Positively charged amino acids at the SNAP-25 C terminus determine fusion rates, fusion pore properties, and energetics of tight SNARE complex zippering.
Fang Q, Zhao Y, Herbst AD, Kim BN, Lindau M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 357: 3230-9. 111 111 ICC; tested species: cow
Aberrant function and structure of retinal ribbon synapses in the absence of complexin 3 and complexin 4.
Reim K, Regus-Leidig H, Ammermüller J, El-Kordi A, Radyushkin K, Ehrenreich H, Brandstätter JH, Brose N
Journal of cell science (2009) 122Pt 9: 1352-61. 111 111 WB, IHC; tested species: mouse
The expression pattern and assembly profile of synaptic membrane proteins in ribbon synapses of the developing mouse retina.
von Kriegstein K, Schmitz F
Cell and tissue research (2003) 3112: 159-73. 111 111 IHC
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 111 111 DNA-PAINT; tested species: rat
Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission.
Vasileva M, Horstmann H, Geumann C, Gitler D, Kuner T
The European journal of neuroscience (2012) 368: 3005-20. 111 111 ELISA
Inhibition of SNARE complex assembly differentially affects kinetic components of exocytosis.
Xu T, Rammner B, Margittai M, Artalejo AR, Neher E, Jahn R
Cell (1999) 997: 713-22. 111 111
Cat. No.: 111 111
Amount: 50 µg
Price: $430.00
Proximity labelling reveals effects of disease-causing mutation on the DNAJC5/cysteine string protein α interactome.
Barker E, Milburn A, Helassa N, Hammond D, Sanchez-Soriano N, Morgan A, Barclay J
The Biochemical journal (2024) : . 111 111 WB; tested species: rat
LRRTMs Organize Synapses through Differential Engagement of Neurexin and PTPσ.
Roppongi RT, Dhume SH, Padmanabhan N, Silwal P, Zahra N, Karimi B, Bomkamp C, Patil CS, Champagne-Jorgensen K, Twilley RE, Zhang P, et al.
Neuron (2020) : . 111 111 WB; tested species: mouse
VAMP4 maintains a Ca2+-sensitive pool of spontaneously recycling synaptic vesicles.
Lin PY, Chanaday NL, Horvath PM, Ramirez DMO, Monteggia LM, Kavalali ET
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 111 111 WB; tested species: rat
Potential Protection Against Type 2 Diabetes in Obesity Through Lower CD36 Expression and Improved Exocytosis in β-Cells.
Nagao M, Esguerra JLS, Asai A, Ofori JK, Edlund A, Wendt A, Sugihara H, Wollheim CB, Oikawa S, Eliasson L
Diabetes (2020) 696: 1193-1205. 111 111 WB; tested species: human
Role of Aberrant Spontaneous Neurotransmission in SNAP25-Associated Encephalopathies.
Alten B, Zhou Q, Shin OH, Esquivies L, Lin PY, White KI, Sun R, Chung WK, Monteggia LM, Brunger AT, Kavalali ET, et al.
Neuron (2020) : . 111 111 WB; tested species: mouse
Intracellular Vesicle Fusion Requires a Membrane-Destabilizing Peptide Located at the Juxtamembrane Region of the v-SNARE.
Rathore SS, Liu Y, Yu H, Wan C, Lee M, Yin Q, Stowell MHB, Shen J
Cell reports (2019) 2913: 4583-4592.e3. 111 111 WB
Phosphorylated SNAP25 in the CA1 regulates morphine-associated contextual memory retrieval via increasing GluN2B-NMDAR surface localization.
Wang X, Liu Y, Jia M, Sun X, Wang N, Li Y, Cui C
Addiction biology (2017) : . 111 111 WB; tested species: rat
Identification and characterization of a novel botulinum neurotoxin.
Zhang S, Masuyer G, Zhang J, Shen Y, Lundin D, Henriksson L, Miyashita SI, Martínez-Carranza M, Dong M, Stenmark P
Nature communications (2017) 8: 14130. 111 111 WB; tested species: mouse
The neural cell adhesion molecule promotes maturation of the presynaptic endocytotic machinery by switching synaptic vesicle recycling from adaptor protein 3 (AP-3)- to AP-2-dependent mechanisms.
Shetty A, Sytnyk V, Leshchyns'ka I, Puchkov D, Haucke V, Schachner M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3342: 16828-45. 111 111 WB
Small-scale isolation of synaptic vesicles from mammalian brain.
Ahmed S, Holt M, Riedel D, Jahn R
Nature protocols (2013) 85: 998-1009. 111 111 WB; tested species: mouse
Aberrant function and structure of retinal ribbon synapses in the absence of complexin 3 and complexin 4.
Reim K, Regus-Leidig H, Ammermüller J, El-Kordi A, Radyushkin K, Ehrenreich H, Brandstätter JH, Brose N
Journal of cell science (2009) 122Pt 9: 1352-61. 111 111 WB, IHC; tested species: mouse
Dvl regulates endo- and exocytotic processes through binding to synaptotagmin.
Kishida S, Hamao K, Inoue M, Hasegawa M, Matsuura Y, Mikoshiba K, Fukuda M, Kikuchi A
Genes to cells : devoted to molecular & cellular mechanisms (2007) 121: 49-61. 111 111 WB; tested species: rat
Loss of SNAP-25 and rabphilin 3a in sensory-motor cortex in Huntington's disease.
Smith R, Klein P, Koc-Schmitz Y, Waldvogel HJ, Faull RL, Brundin P, Plomann M, Li JY
Journal of neurochemistry (2007) 1031: 115-23. 111 111 WB; tested species: human
Rapid and Sensitive Nano-Immunosensors for Botulinum.
Cheng HP, Chuang HS
ACS sensors (2019) : . 111 111 IP; tested species: human
The N-ethylmaleimide-sensitive fusion protein and alpha-SNAP induce a conformational change in syntaxin.
Hanson PI, Otto H, Barton N, Jahn R
The Journal of biological chemistry (1995) 27028: 16955-61. 111 111 IP
Positively charged amino acids at the SNAP-25 C terminus determine fusion rates, fusion pore properties, and energetics of tight SNARE complex zippering.
Fang Q, Zhao Y, Herbst AD, Kim BN, Lindau M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 357: 3230-9. 111 111 ICC; tested species: cow
Aberrant function and structure of retinal ribbon synapses in the absence of complexin 3 and complexin 4.
Reim K, Regus-Leidig H, Ammermüller J, El-Kordi A, Radyushkin K, Ehrenreich H, Brandstätter JH, Brose N
Journal of cell science (2009) 122Pt 9: 1352-61. 111 111 WB, IHC; tested species: mouse
The expression pattern and assembly profile of synaptic membrane proteins in ribbon synapses of the developing mouse retina.
von Kriegstein K, Schmitz F
Cell and tissue research (2003) 3112: 159-73. 111 111 IHC
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 111 111 DNA-PAINT; tested species: rat
Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission.
Vasileva M, Horstmann H, Geumann C, Gitler D, Kuner T
The European journal of neuroscience (2012) 368: 3005-20. 111 111 ELISA
Inhibition of SNARE complex assembly differentially affects kinetic components of exocytosis.
Xu T, Rammner B, Margittai M, Artalejo AR, Neher E, Jahn R
Cell (1999) 997: 713-22. 111 111
WB
ICC
IHC
IHC-P
Background

SNAP 25 (synaptosome-associated protein of 25 kDa) is a highly conserved protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP 25 is the target of Botulinum neurotoxin A and E which cleave off 9 and 26 amino acids, respectively, from the C-terminus.
SNAP 25 is part of the exocytotic fusion complex (v-SNARE) of neurons where it assembles with syntaxin 1 and synaptobrevin. It is abundantly localized on the neuronal plasmalemma and on recycling vesicles including synaptic vesicles. It is also expressed in neuroendocrine cells. There are two splice-variants, SNAP 25A and 25B.
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