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SNAP25 antibody - 111 011BT

SNAP25 is a palmitoylated plasma membrane protein; required for exocytosis
Mouse monoclonal purified IgG
Cat. No.: 111 011BT
Amount: 50 µg
Price: $465.00
Cat. No. 111 011BT 50 µg purified IgG, lyophilized, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery  
IP: yes (see remarks)
ICC: 1 : 100 up to 1 : 1000
IHC: not recommended
IHC-P: not tested yet
Label biotin
Clone 71.1
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 206 from rat SNAP25B (UniProt Id: P60881-1)
Epitop Epitop: AA 20 to 40 from rat SNAP25B (UniProt Id: P60881-1)
Reactivity Reacts with: human (P60880), rat (P60881), mouse (P60879), vertebrates, invertebrates.
Other species not tested yet.
Specificity Detects both splice variants SNAP 25A and B. Recognizes the Botulinum neurotoxin A and E cleavage products.
Remarks

IP: Immunoprecipitation not quantitative, appears to depend on the binding status of the protein.

Data sheet 111_011bt.pdf
Cat. No.: 111 011BT
Amount: 50 µg
Price: $465.00
Background

SNAP 25 (synaptosome-associated protein of 25 kDa) is a highly conserved protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP 25 is the target of Botulinum neurotoxin A and E which cleave off 9 and 26 amino acids, respectively, from the C-terminus.
SNAP 25 is part of the exocytotic fusion complex (v-SNARE) of neurons where it assembles with syntaxin 1 and synaptobrevin. It is abundantly localized on the neuronal plasmalemma and on recycling vesicles including synaptic vesicles. It is also expressed in neuroendocrine cells. There are two splice-variants, SNAP 25A and 25B.