Tailor-made Antibodies
and Tools for Life Science
quick order | cart
?
|
login
Home|||||Technical Support
Home > Products > 111 011

SNAP25 antibody - 111 011 K.O.

SNAP25 is a palmitoylated plasma membrane protein; required for exocytosis
Mouse monoclonal purified IgG
Cat. No.: 111 011
Amount: 50 µg
Price: $430.00
Cat. No. 111 011 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery  
IP: yes (see remarks)
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: not recommended
IHC-P: 1 : 2000 gallery  
EM: yes
Clone 71.1
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 206 from rat SNAP25B (UniProt Id: P60881-1)
Epitop Epitop: AA 20 to 40 from rat SNAP25B (UniProt Id: P60881-1)
Reactivity Reacts with: human (P60880), rat (P60881), mouse (P60879), vertebrates, invertebrates, zebrafish.
Other species not tested yet.
Specificity Detects both splice variants SNAP 25A and B. Recognizes the Botulinum neurotoxin A and E cleavage products. K.O. validated PubMed: 31794878
Remarks

IP: Immunoprecipitation not quantitative, appears to depend on the binding status of the protein.
Data sheet 111_011.pdf

References for SNAP25 - 111 011

Neurotransmitter release progressively desynchronizes in induced human neurons during synapse maturation and aging.
Uzay B, Houcek A, Ma ZZ, Konradi C, Monteggia LM, Kavalali ET
Cell reports (2023) 422: 112042. 111 011 WB; tested species: human
Glutamatergic synapses from the insular cortex to the basolateral amygdala encode observational pain.
Zhang MM, Geng AQ, Chen K, Wang J, Wang P, Qiu XT, Gu JX, Fan HW, Zhu DY, Yang SM, Chen QY, et al.
Neuron (2022) 11012: 1993-2008.e6. 111 011 WB; tested species: mouse
Selective endocytosis of Ca2+-permeable AMPARs by the Alzheimer's disease risk factor CALM bidirectionally controls synaptic plasticity.
Azarnia Tehran D, Kochlamazashvili G, Pampaloni NP, Sposini S, Shergill JK, Lehmann M, Pashkova N, Schmidt C, Löwe D, Napieczynska H, Heuser A, et al.
Science advances (2022) 821: eabl5032. 111 011 WB; tested species: mouse
Endosomal phosphatidylinositol 3-phosphate controls synaptic vesicle cycling and neurotransmission.
Liu GT, Kochlamazashvili G, Puchkov D, Müller R, Schultz C, Mackintosh AI, Vollweiter D, Haucke V, Soykan T
The EMBO journal (2022) : e109352. 111 011 WB; tested species: rat
Human Islet MicroRNA-200c Is Elevated in Type 2 Diabetes and Targets the Transcription Factor ETV5 to Reduce Insulin Secretion.
Ofori JK, Karagiannopoulos A, Nagao M, Westholm E, Ramadan S, Wendt A, Esguerra JLS, Eliasson L
Diabetes (2022) 712: 275-284. 111 011 WB; tested species: human
Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes.
Ivanova D, Dobson KL, Gajbhiye A, Davenport EC, Hacker D, Ultanir SK, Trost M, Cousin MA
Science advances (2021) 718: . 111 011 WB; tested species: mouse
Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.
Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht MT, Krause E, Maritzen T, Schmitz D, Haucke V
Neuron (2021) 1092: 299-313.e9. 111 011 WB; tested species: mouse
Sirtuin 6 is a regulator of dendrite morphogenesis in rat hippocampal neurons.
Matsuno H, Tsuchimine S, Fukuzato N, O'Hashi K, Kunugi H, Sohya K
Neurochemistry international (2021) : 104959. 111 011 WB; tested species: rat
The phosphoprotein Synapsin Ia regulates the kinetics of dense-core vesicle release.
Yang HJ, Chen PC, Huang CT, Cheng TL, Hsu SP, Chen CY, Lu JC, Wang CT
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) : . 111 011 WB, ICC; tested species: rat
Synaptotagmin-1 interacts with PI(4,5)P2 to initiate synaptic vesicle docking in hippocampal neurons.
Chen Y, Wang YH, Zheng Y, Li M, Wang B, Wang QW, Fu CL, Liu YN, Li X, Yao J
Cell reports (2021) 3411: 108842. 111 011 WB; KD verified; tested species: mouse
Munc13-1 is a Ca2+-phospholipid-dependent vesicle priming hub that shapes synaptic short-term plasticity and enables sustained neurotransmission.
Lipstein N, Chang S, Lin KH, López-Murcia FJ, Neher E, Taschenberger H, Brose N
Neuron (2021) : . 111 011 WB; tested species: mouse
Prenatal interleukin 6 elevation increases glutamatergic synapse density and disrupts hippocampal connectivity in offspring.
Mirabella F, Desiato G, Mancinelli S, Fossati G, Rasile M, Morini R, Markicevic M, Grimm C, Amegandjin C, Termanini A, Peano C, et al.
Immunity (2021) 5411: 2611-2631.e8. 111 011 WB; tested species: mouse
RTN4/NoGo-receptor binding to BAI adhesion-GPCRs regulates neuronal development.
Wang J, Miao Y, Wicklein R, Sun Z, Wang J, Jude KM, Fernandes RA, Merrill SA, Wernig M, Garcia KC, Südhof TC, et al.
Cell (2021) 18424: 5869-5885.e25. 111 011 WB; tested species: human
TRIM67 regulates exocytic mode and neuronal morphogenesis via SNAP47.
Urbina FL, Menon S, Goldfarb D, Edwards R, Ben Major M, Brennwald P, Gupton SL
Cell reports (2021) 346: 108743. 111 011 WB; tested species: mouse
A Catch and Anchor Approach to Combat both Toxicity and Longevity of Botulinum Toxin A.
Lin L, Olson ME, Sugane T, Turner LD, Tararina MA, Nielsen AL, Kurbanov EK, Pellett S, Johnson EA, Cohen SM, Allen KN, et al.
Journal of medicinal chemistry (2020) : . 111 011 WB; tested species: human
Bassoon inhibits proteasome activity via interaction with PSMB4.
Montenegro-Venegas C, Fienko S, Anni D, Pina-Fernández E, Frischknecht R, Fejtova A
Cellular and molecular life sciences : CMLS (2020) : . 111 011 WB; tested species: mouse
Changes in the Expression of SNAP-25 Protein in the Brain of Juvenile Rats in Two Models of Autism.
Lenart J, Bratek E, Lazarewicz JW, Zieminska E
Journal of molecular neuroscience : MN (2020) : . 111 011 WB; tested species: rat
Actions of Rab27B-GTPase on mammalian central excitatory synaptic transmission.
Arias-Hervert ER, Xu N, Njus M, Murphy GG, Hou Y, Williams JA, Lentz SI, Ernst SA, Stuenkel EL
Physiological reports (2020) 89: e14428. 111 011 WB; tested species: mouse
Endophilin-A coordinates priming and fusion of neurosecretory vesicles via intersectin.
Gowrisankaran S, Houy S, Del Castillo JGP, Steubler V, Gelker M, Kroll J, Pinheiro PS, Schwitters D, Halbsgut N, Pechstein A, van Weering JRT, et al.
Nature communications (2020) 111: 1266. 111 011 WB, ICC; tested species: mouse
Aggregation of mutant cysteine string protein-α via Fe-S cluster binding is mitigated by iron chelators.
Naseri NN, Ergel B, Kharel P, Na Y, Huang Q, Huang R, Dolzhanskaya N, Burré J, Velinov MT, Sharma M
Nature structural & molecular biology (2020) 272: 192-201. 111 011 WB, IP; tested species: mouse
Fluoxetine Suppresses Glutamate- and GABA-Mediated Neurotransmission by Altering SNARE Complex.
Lazarevic V, Mantas I, Flais I, Svenningsson P
International journal of molecular sciences (2019) 2017: . 111 011 WB; tested species: rat
Elevated synaptic vesicle release probability in synaptophysin/gyrin family quadruple knockouts.
Raja MK, Preobraschenski J, Del Olmo-Cabrera S, Martinez-Turrillas R, Jahn R, Perez-Otano I, Wesseling JF
eLife (2019) 8: . 111 011 WB; tested species: mouse
SNAP-25 phosphorylation at Ser187 is not involved in Ca2+ or phorbolester-dependent potentiation of synaptic release.
Ruiter M, Houy S, Engholm-Keller K, Graham ME, Sørensen JB
Molecular and cellular neurosciences (2019) : 103452. 111 011 WB; KO verified; tested species: mouse
An Electrostatic Energy Barrier for SNARE-Dependent Spontaneous and Evoked Synaptic Transmission.
Ruiter M, Kádková A, Scheutzow A, Malsam J, Söllner TH, Sørensen JB
Cell reports (2019) 269: 2340-2352.e5. 111 011 WB; tested species: mouse
Identification of a Botulinum Neurotoxin-like Toxin in a Commensal Strain of Enterococcus faecium.
Zhang S, Lebreton F, Mansfield MJ, Miyashita SI, Zhang J, Schwartzman JA, Tao L, Masuyer G, Martínez-Carranza M, Stenmark P, Gilmore MS, et al.
Cell host & microbe (2018) 232: 169-176.e6. 111 011 WB; tested species: rat
Covalent modifiers of Botulinum neurotoxin counteract toxin persistence.
Garland M, Babin BM, Miyashita SI, Loscher S, Shen Y, Dong M, Bogyo M
ACS chemical biology (2018) : . 111 011 WB
Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting.
Awasthi A, Ramachandran B, Ahmed S, Benito E, Shinoda Y, Nitzan N, Heukamp A, Rannio S, Martens H, Barth J, Burk K, et al.
Science (New York, N.Y.) (2018) : . 111 011 WB; tested species: rat
Synaptotagmin oligomerization is essential for calcium control of regulated exocytosis.
Bello OD, Jouannot O, Chaudhuri A, Stroeva E, Coleman J, Volynski KE, Rothman JE, Krishnakumar SS
Proceedings of the National Academy of Sciences of the United States of America (2018) 11532: E7624-E7631. 111 011 WB; tested species: rat
CRISPR interference-based specific and efficient gene inactivation in the brain.
Zheng Y, Shen W, Zhang J, Yang B, Liu YN, Qi H, Yu X, Lu SY, Chen Y, Xu YZ, Li Y, et al.
Nature neuroscience (2018) 213: 447-454. 111 011 WB; KD verified; tested species: mouse
CaV2.2 Gates Calcium-Independent but Voltage-Dependent Secretion in Mammalian Sensory Neurons.
Chai Z, Wang C, Huang R, Wang Y, Zhang X, Wu Q, Wang Y, Wu X, Zheng L, Zhang C, Guo W, et al.
Neuron (2017) 966: 1317-1326.e4. 111 011 WB, IP, ICC; tested species: rat
MiR-335 overexpression impairs insulin secretion through defective priming of insulin vesicles.
Salunkhe VA, Ofori JK, Gandasi NR, Salö SA, Hansson S, Andersson ME, Wendt A, Barg S, Esguerra JLS, Eliasson L
Physiological reports (2017) 521: . 111 011 WB; tested species: rat
Plekhg5-regulated autophagy of synaptic vesicles reveals a pathogenic mechanism in motoneuron disease.
Lüningschrör P, Binotti B, Dombert B, Heimann P, Perez-Lara A, Slotta C, Thau-Habermann N, R von Collenberg C, Karl F, Damme M, Horowitz A, et al.
Nature communications (2017) 81: 678. 111 011 WB
A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 111 011 WB
Olanzapine Reverses MK-801-Induced Cognitive Deficits and Region-Specific Alterations of NMDA Receptor Subunits.
Liu X, Li J, Guo C, Wang H, Sun Y, Wang H, Su YA, Li K, Si T
Frontiers in behavioral neuroscience (2017) 11: 260. 111 011 WB; tested species: rat
Impaired AMPA receptor trafficking by a double knockout of zebrafish olfactomedin1a/b.
Nakaya N, Sultana A, Tomarev SI
Journal of neurochemistry (2017) 1436: 635-644. 111 011 WB; tested species: zebrafish
Activity-Dependent Degradation of Synaptic Vesicle Proteins Requires Rab35 and the ESCRT Pathway.
Sheehan P, Zhu M, Beskow A, Vollmer C, Waites CL
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3633: 8668-86. 111 011 WB; tested species: rat
How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 111 011 WB
Complexin stabilizes newly primed synaptic vesicles and prevents their premature fusion at the mouse calyx of held synapse.
Chang S, Reim K, Pedersen M, Neher E, Brose N, Taschenberger H
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3521: 8272-90. 111 011 WB
Wnt signalling tunes neurotransmitter release by directly targeting Synaptotagmin-1.
Ciani L, Marzo A, Boyle K, Stamatakou E, Lopes DM, Anane D, McLeod F, Rosso SB, Gibb A, Salinas PC
Nature communications (2015) 6: 8302. 111 011 WB
Ubiquitin-Synaptobrevin Fusion Protein Causes Degeneration of Presynaptic Motor Terminals in Mice.
Liu Y, Li H, Sugiura Y, Han W, Gallardo G, Khvotchev M, Zhang Y, Kavalali ET, Südhof TC, Lin W
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3533: 11514-31. 111 011 WB
Vesicular Synaptobrevin/VAMP2 Levels Guarded by AP180 Control Efficient Neurotransmission.
Koo SJ, Kochlamazashvili G, Rost B, Puchkov D, Gimber N, Lehmann M, Tadeus G, Schmoranzer J, Rosenmund C, Haucke V, Maritzen T, et al.
Neuron (2015) 882: 330-44. 111 011 WB; tested species: mouse
Synaptotagmin-1 and -7 Are Redundantly Essential for Maintaining the Capacity of the Readily-Releasable Pool of Synaptic Vesicles.
Bacaj T, Wu D, Burré J, Malenka RC, Liu X, Südhof TC
PLoS biology (2015) 1310: e1002267. 111 011 WB
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 111 011 ICC, WB; tested species: rat
α-Synuclein assembles into higher-order multimers upon membrane binding to promote SNARE complex formation.
Burré J, Sharma M, Südhof TC
Proceedings of the National Academy of Sciences of the United States of America (2014) 11140: E4274-83. 111 011 WB
Phosphorylation of syntaxin 3B by CaMKII regulates the formation of t-SNARE complexes.
Liu X, Heidelberger R, Janz R
Molecular and cellular neurosciences (2014) 60: 53-62. 111 011 WB; tested species: mouse
C-terminal complexin sequence is selectively required for clamping and priming but not for Ca2+ triggering of synaptic exocytosis.
Kaeser-Woo YJ, Yang X, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 328: 2877-85. 111 011 WB; tested species: rat
CSPα promotes SNARE-complex assembly by chaperoning SNAP-25 during synaptic activity.
Sharma M, Burré J, Südhof TC
Nature cell biology (2011) 131: 30-9. 111 011 WB; tested species: mouse
SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 111 011 WB, ICC
SV2 mediates entry of tetanus neurotoxin into central neurons.
Yeh FL, Dong M, Yao J, Tepp WH, Lin G, Johnson EA, Chapman ER
PLoS pathogens (2010) 611: e1001207. 111 011 WB; tested species: mouse
SV2B regulates synaptotagmin 1 by direct interaction.
Lazzell DR, Belizaire R, Thakur P, Sherry DM, Janz R
The Journal of biological chemistry (2004) 27950: 52124-31. 111 011 WB; tested species: mouse
Regulation of releasable vesicle pool sizes by protein kinase A-dependent phosphorylation of SNAP-25.
Nagy G, Reim K, Matti U, Brose N, Binz T, Rettig J, Neher E, Sørensen JB
Neuron (2004) 413: 417-29. 111 011 WB
An essential role for vesicular glutamate transporter 1 (VGLUT1) in postnatal development and control of quantal size.
Wojcik SM, Rhee JS, Herzog E, Sigler A, Jahn R, Takamori S, Brose N, Rosenmund C
Proceedings of the National Academy of Sciences of the United States of America (2004) 10118: 7158-63. 111 011 WB; tested species: mouse
Synaptic biomarkers in the cerebrospinal fluid associate differentially with classical neuronal biomarkers in patients with Alzheimer's disease and frontotemporal dementia.
Das S, Goossens J, Jacobs D, Dewit N, Pijnenburg YAL, In 't Veld SGJG, Teunissen CE, Vanmechelen E
Alzheimer's research & therapy (2023) 151: 62. 111 011 IP; tested species: human
Aggregation of mutant cysteine string protein-α via Fe-S cluster binding is mitigated by iron chelators.
Naseri NN, Ergel B, Kharel P, Na Y, Huang Q, Huang R, Dolzhanskaya N, Burré J, Velinov MT, Sharma M
Nature structural & molecular biology (2020) 272: 192-201. 111 011 WB, IP; tested species: mouse
Rapid and Sensitive Nano-Immunosensors for Botulinum.
Cheng HP, Chuang HS
ACS sensors (2019) : . 111 011 IP; tested species: human
CaV2.2 Gates Calcium-Independent but Voltage-Dependent Secretion in Mammalian Sensory Neurons.
Chai Z, Wang C, Huang R, Wang Y, Zhang X, Wu Q, Wang Y, Wu X, Zheng L, Zhang C, Guo W, et al.
Neuron (2017) 966: 1317-1326.e4. 111 011 WB, IP, ICC; tested species: rat
α-Synuclein induced cholesterol lowering increases tonic and reduces depolarization-evoked synaptic vesicle recycling and glutamate release.
Lazarevic V, Yang Y, Paslawski W, Svenningsson P
NPJ Parkinson's disease (2022) 81: 71. 111 011 ICC; tested species: mouse
Synaptotagmin-7-mediated activation of spontaneous NMDAR currents is disrupted in bipolar disorder susceptibility variants.
Wang QW, Wang YH, Wang B, Chen Y, Lu SY, Yao J
PLoS biology (2021) 197: e3001323. 111 011 ICC; tested species: mouse
The phosphoprotein Synapsin Ia regulates the kinetics of dense-core vesicle release.
Yang HJ, Chen PC, Huang CT, Cheng TL, Hsu SP, Chen CY, Lu JC, Wang CT
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) : . 111 011 WB, ICC; tested species: rat
Endophilin-A coordinates priming and fusion of neurosecretory vesicles via intersectin.
Gowrisankaran S, Houy S, Del Castillo JGP, Steubler V, Gelker M, Kroll J, Pinheiro PS, Schwitters D, Halbsgut N, Pechstein A, van Weering JRT, et al.
Nature communications (2020) 111: 1266. 111 011 WB, ICC; tested species: mouse
Intersectin-Mediated Clearance of SNARE Complexes Is Required for Fast Neurotransmission.
Jäpel M, Gerth F, Sakaba T, Bacetic J, Yao L, Koo SJ, Maritzen T, Freund C, Haucke V
Cell reports (2020) 302: 409-420.e6. 111 011 ICC; tested species: mouse
Riluzole attenuates the efficacy of glutamatergic transmission by interfering with the size of the readily releasable neurotransmitter pool.
Lazarevic V, Yang Y, Ivanova D, Fejtova A, Svenningsson P
Neuropharmacology (2018) : . 111 011 ICC; tested species: rat
CaV2.2 Gates Calcium-Independent but Voltage-Dependent Secretion in Mammalian Sensory Neurons.
Chai Z, Wang C, Huang R, Wang Y, Zhang X, Wu Q, Wang Y, Wu X, Zheng L, Zhang C, Guo W, et al.
Neuron (2017) 966: 1317-1326.e4. 111 011 WB, IP, ICC; tested species: rat
Atoxic derivative of botulinum neurotoxin A as a prototype molecular vehicle for targeted delivery to the neuronal cytoplasm.
Vazquez-Cintron EJ, Vakulenko M, Band PA, Stanker LH, Johnson EA, Ichtchenko K
PloS one (2014) 91: e85517. 111 011 ICC; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 111 011 ICC, WB; tested species: rat
Synaptotagmin interaction with SNAP-25 governs vesicle docking, priming, and fusion triggering.
Mohrmann R, de Wit H, Connell E, Pinheiro PS, Leese C, Bruns D, Davletov B, Verhage M, Sørensen JB
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3336: 14417-30. 111 011 ICC
SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 111 011 WB, ICC
Temporal characteristics of vesicular fusion in astrocytes: examination of synaptobrevin 2-laden vesicles at single vesicle resolution.
Malarkey EB, Parpura V
The Journal of physiology (2011) 58917: 4271-300. 111 011 ICC; tested species: rat
Nanobodies reveal an extra-synaptic population of SNAP-25 and Syntaxin 1A in hippocampal neurons.
Maidorn M, Olichon A, Rizzoli SO, Opazo F
mAbs () 112: 305-321. 111 011 ICC; tested species: rat
Hemisynapse Formation Between Target Astrocytes and Cortical Neuron Axons in vitro.
Teng Z, Gottmann K
Frontiers in molecular neuroscience (2022) 15: 829506. 111 011 IHC; tested species: mouse
The Isolated Mouse Jejunal Afferent Nerve Assay as a Tool to Assess the Effect of Botulinum Neurotoxins in Visceral Nociception.
Retailleau K, Martin V, Lezmi S, Nicoleau C, Maignel J
Toxins (2022) 143: . 111 011 IHC; tested species: mouse
Recombinant botulinum neurotoxin serotype A1 in vivo characterization.
Périer C, Martin V, Cornet S, Favre-Guilmard C, Rocher MN, Bindler J, Wagner S, Andriambeloson E, Rudkin B, Marty R, Vignaud A, et al.
Pharmacology research & perspectives (2021) 95: e00857. 111 011 IHC; tested species: mouse
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 111 011 IHC; tested species: rat
Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells.
Kirizs T, Kerti-Szigeti K, Lorincz A, Nusser Z
The European journal of neuroscience (2014) 3911: 1771-83. 111 011 EM; tested species: rat
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 111 011
Cat. No.: 111 011
Amount: 50 µg
Price: $430.00
Neurotransmitter release progressively desynchronizes in induced human neurons during synapse maturation and aging.
Uzay B, Houcek A, Ma ZZ, Konradi C, Monteggia LM, Kavalali ET
Cell reports (2023) 422: 112042. 111 011 WB; tested species: human
Glutamatergic synapses from the insular cortex to the basolateral amygdala encode observational pain.
Zhang MM, Geng AQ, Chen K, Wang J, Wang P, Qiu XT, Gu JX, Fan HW, Zhu DY, Yang SM, Chen QY, et al.
Neuron (2022) 11012: 1993-2008.e6. 111 011 WB; tested species: mouse
Selective endocytosis of Ca2+-permeable AMPARs by the Alzheimer's disease risk factor CALM bidirectionally controls synaptic plasticity.
Azarnia Tehran D, Kochlamazashvili G, Pampaloni NP, Sposini S, Shergill JK, Lehmann M, Pashkova N, Schmidt C, Löwe D, Napieczynska H, Heuser A, et al.
Science advances (2022) 821: eabl5032. 111 011 WB; tested species: mouse
Endosomal phosphatidylinositol 3-phosphate controls synaptic vesicle cycling and neurotransmission.
Liu GT, Kochlamazashvili G, Puchkov D, Müller R, Schultz C, Mackintosh AI, Vollweiter D, Haucke V, Soykan T
The EMBO journal (2022) : e109352. 111 011 WB; tested species: rat
Human Islet MicroRNA-200c Is Elevated in Type 2 Diabetes and Targets the Transcription Factor ETV5 to Reduce Insulin Secretion.
Ofori JK, Karagiannopoulos A, Nagao M, Westholm E, Ramadan S, Wendt A, Esguerra JLS, Eliasson L
Diabetes (2022) 712: 275-284. 111 011 WB; tested species: human
Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes.
Ivanova D, Dobson KL, Gajbhiye A, Davenport EC, Hacker D, Ultanir SK, Trost M, Cousin MA
Science advances (2021) 718: . 111 011 WB; tested species: mouse
Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.
Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht MT, Krause E, Maritzen T, Schmitz D, Haucke V
Neuron (2021) 1092: 299-313.e9. 111 011 WB; tested species: mouse
Sirtuin 6 is a regulator of dendrite morphogenesis in rat hippocampal neurons.
Matsuno H, Tsuchimine S, Fukuzato N, O'Hashi K, Kunugi H, Sohya K
Neurochemistry international (2021) : 104959. 111 011 WB; tested species: rat
The phosphoprotein Synapsin Ia regulates the kinetics of dense-core vesicle release.
Yang HJ, Chen PC, Huang CT, Cheng TL, Hsu SP, Chen CY, Lu JC, Wang CT
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) : . 111 011 WB, ICC; tested species: rat
Synaptotagmin-1 interacts with PI(4,5)P2 to initiate synaptic vesicle docking in hippocampal neurons.
Chen Y, Wang YH, Zheng Y, Li M, Wang B, Wang QW, Fu CL, Liu YN, Li X, Yao J
Cell reports (2021) 3411: 108842. 111 011 WB; KD verified; tested species: mouse
Munc13-1 is a Ca2+-phospholipid-dependent vesicle priming hub that shapes synaptic short-term plasticity and enables sustained neurotransmission.
Lipstein N, Chang S, Lin KH, López-Murcia FJ, Neher E, Taschenberger H, Brose N
Neuron (2021) : . 111 011 WB; tested species: mouse
Prenatal interleukin 6 elevation increases glutamatergic synapse density and disrupts hippocampal connectivity in offspring.
Mirabella F, Desiato G, Mancinelli S, Fossati G, Rasile M, Morini R, Markicevic M, Grimm C, Amegandjin C, Termanini A, Peano C, et al.
Immunity (2021) 5411: 2611-2631.e8. 111 011 WB; tested species: mouse
RTN4/NoGo-receptor binding to BAI adhesion-GPCRs regulates neuronal development.
Wang J, Miao Y, Wicklein R, Sun Z, Wang J, Jude KM, Fernandes RA, Merrill SA, Wernig M, Garcia KC, Südhof TC, et al.
Cell (2021) 18424: 5869-5885.e25. 111 011 WB; tested species: human
TRIM67 regulates exocytic mode and neuronal morphogenesis via SNAP47.
Urbina FL, Menon S, Goldfarb D, Edwards R, Ben Major M, Brennwald P, Gupton SL
Cell reports (2021) 346: 108743. 111 011 WB; tested species: mouse
A Catch and Anchor Approach to Combat both Toxicity and Longevity of Botulinum Toxin A.
Lin L, Olson ME, Sugane T, Turner LD, Tararina MA, Nielsen AL, Kurbanov EK, Pellett S, Johnson EA, Cohen SM, Allen KN, et al.
Journal of medicinal chemistry (2020) : . 111 011 WB; tested species: human
Bassoon inhibits proteasome activity via interaction with PSMB4.
Montenegro-Venegas C, Fienko S, Anni D, Pina-Fernández E, Frischknecht R, Fejtova A
Cellular and molecular life sciences : CMLS (2020) : . 111 011 WB; tested species: mouse
Changes in the Expression of SNAP-25 Protein in the Brain of Juvenile Rats in Two Models of Autism.
Lenart J, Bratek E, Lazarewicz JW, Zieminska E
Journal of molecular neuroscience : MN (2020) : . 111 011 WB; tested species: rat
Actions of Rab27B-GTPase on mammalian central excitatory synaptic transmission.
Arias-Hervert ER, Xu N, Njus M, Murphy GG, Hou Y, Williams JA, Lentz SI, Ernst SA, Stuenkel EL
Physiological reports (2020) 89: e14428. 111 011 WB; tested species: mouse
Endophilin-A coordinates priming and fusion of neurosecretory vesicles via intersectin.
Gowrisankaran S, Houy S, Del Castillo JGP, Steubler V, Gelker M, Kroll J, Pinheiro PS, Schwitters D, Halbsgut N, Pechstein A, van Weering JRT, et al.
Nature communications (2020) 111: 1266. 111 011 WB, ICC; tested species: mouse
Aggregation of mutant cysteine string protein-α via Fe-S cluster binding is mitigated by iron chelators.
Naseri NN, Ergel B, Kharel P, Na Y, Huang Q, Huang R, Dolzhanskaya N, Burré J, Velinov MT, Sharma M
Nature structural & molecular biology (2020) 272: 192-201. 111 011 WB, IP; tested species: mouse
Fluoxetine Suppresses Glutamate- and GABA-Mediated Neurotransmission by Altering SNARE Complex.
Lazarevic V, Mantas I, Flais I, Svenningsson P
International journal of molecular sciences (2019) 2017: . 111 011 WB; tested species: rat
Elevated synaptic vesicle release probability in synaptophysin/gyrin family quadruple knockouts.
Raja MK, Preobraschenski J, Del Olmo-Cabrera S, Martinez-Turrillas R, Jahn R, Perez-Otano I, Wesseling JF
eLife (2019) 8: . 111 011 WB; tested species: mouse
SNAP-25 phosphorylation at Ser187 is not involved in Ca2+ or phorbolester-dependent potentiation of synaptic release.
Ruiter M, Houy S, Engholm-Keller K, Graham ME, Sørensen JB
Molecular and cellular neurosciences (2019) : 103452. 111 011 WB; KO verified; tested species: mouse
An Electrostatic Energy Barrier for SNARE-Dependent Spontaneous and Evoked Synaptic Transmission.
Ruiter M, Kádková A, Scheutzow A, Malsam J, Söllner TH, Sørensen JB
Cell reports (2019) 269: 2340-2352.e5. 111 011 WB; tested species: mouse
Identification of a Botulinum Neurotoxin-like Toxin in a Commensal Strain of Enterococcus faecium.
Zhang S, Lebreton F, Mansfield MJ, Miyashita SI, Zhang J, Schwartzman JA, Tao L, Masuyer G, Martínez-Carranza M, Stenmark P, Gilmore MS, et al.
Cell host & microbe (2018) 232: 169-176.e6. 111 011 WB; tested species: rat
Covalent modifiers of Botulinum neurotoxin counteract toxin persistence.
Garland M, Babin BM, Miyashita SI, Loscher S, Shen Y, Dong M, Bogyo M
ACS chemical biology (2018) : . 111 011 WB
Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting.
Awasthi A, Ramachandran B, Ahmed S, Benito E, Shinoda Y, Nitzan N, Heukamp A, Rannio S, Martens H, Barth J, Burk K, et al.
Science (New York, N.Y.) (2018) : . 111 011 WB; tested species: rat
Synaptotagmin oligomerization is essential for calcium control of regulated exocytosis.
Bello OD, Jouannot O, Chaudhuri A, Stroeva E, Coleman J, Volynski KE, Rothman JE, Krishnakumar SS
Proceedings of the National Academy of Sciences of the United States of America (2018) 11532: E7624-E7631. 111 011 WB; tested species: rat
CRISPR interference-based specific and efficient gene inactivation in the brain.
Zheng Y, Shen W, Zhang J, Yang B, Liu YN, Qi H, Yu X, Lu SY, Chen Y, Xu YZ, Li Y, et al.
Nature neuroscience (2018) 213: 447-454. 111 011 WB; KD verified; tested species: mouse
CaV2.2 Gates Calcium-Independent but Voltage-Dependent Secretion in Mammalian Sensory Neurons.
Chai Z, Wang C, Huang R, Wang Y, Zhang X, Wu Q, Wang Y, Wu X, Zheng L, Zhang C, Guo W, et al.
Neuron (2017) 966: 1317-1326.e4. 111 011 WB, IP, ICC; tested species: rat
MiR-335 overexpression impairs insulin secretion through defective priming of insulin vesicles.
Salunkhe VA, Ofori JK, Gandasi NR, Salö SA, Hansson S, Andersson ME, Wendt A, Barg S, Esguerra JLS, Eliasson L
Physiological reports (2017) 521: . 111 011 WB; tested species: rat
Plekhg5-regulated autophagy of synaptic vesicles reveals a pathogenic mechanism in motoneuron disease.
Lüningschrör P, Binotti B, Dombert B, Heimann P, Perez-Lara A, Slotta C, Thau-Habermann N, R von Collenberg C, Karl F, Damme M, Horowitz A, et al.
Nature communications (2017) 81: 678. 111 011 WB
A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 111 011 WB
Olanzapine Reverses MK-801-Induced Cognitive Deficits and Region-Specific Alterations of NMDA Receptor Subunits.
Liu X, Li J, Guo C, Wang H, Sun Y, Wang H, Su YA, Li K, Si T
Frontiers in behavioral neuroscience (2017) 11: 260. 111 011 WB; tested species: rat
Impaired AMPA receptor trafficking by a double knockout of zebrafish olfactomedin1a/b.
Nakaya N, Sultana A, Tomarev SI
Journal of neurochemistry (2017) 1436: 635-644. 111 011 WB; tested species: zebrafish
Activity-Dependent Degradation of Synaptic Vesicle Proteins Requires Rab35 and the ESCRT Pathway.
Sheehan P, Zhu M, Beskow A, Vollmer C, Waites CL
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3633: 8668-86. 111 011 WB; tested species: rat
How to Make an Active Zone: Unexpected Universal Functional Redundancy between RIMs and RIM-BPs.
Acuna C, Liu X, Südhof TC
Neuron (2016) 914: 792-807. 111 011 WB
Complexin stabilizes newly primed synaptic vesicles and prevents their premature fusion at the mouse calyx of held synapse.
Chang S, Reim K, Pedersen M, Neher E, Brose N, Taschenberger H
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3521: 8272-90. 111 011 WB
Wnt signalling tunes neurotransmitter release by directly targeting Synaptotagmin-1.
Ciani L, Marzo A, Boyle K, Stamatakou E, Lopes DM, Anane D, McLeod F, Rosso SB, Gibb A, Salinas PC
Nature communications (2015) 6: 8302. 111 011 WB
Ubiquitin-Synaptobrevin Fusion Protein Causes Degeneration of Presynaptic Motor Terminals in Mice.
Liu Y, Li H, Sugiura Y, Han W, Gallardo G, Khvotchev M, Zhang Y, Kavalali ET, Südhof TC, Lin W
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3533: 11514-31. 111 011 WB
Vesicular Synaptobrevin/VAMP2 Levels Guarded by AP180 Control Efficient Neurotransmission.
Koo SJ, Kochlamazashvili G, Rost B, Puchkov D, Gimber N, Lehmann M, Tadeus G, Schmoranzer J, Rosenmund C, Haucke V, Maritzen T, et al.
Neuron (2015) 882: 330-44. 111 011 WB; tested species: mouse
Synaptotagmin-1 and -7 Are Redundantly Essential for Maintaining the Capacity of the Readily-Releasable Pool of Synaptic Vesicles.
Bacaj T, Wu D, Burré J, Malenka RC, Liu X, Südhof TC
PLoS biology (2015) 1310: e1002267. 111 011 WB
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 111 011 ICC, WB; tested species: rat
α-Synuclein assembles into higher-order multimers upon membrane binding to promote SNARE complex formation.
Burré J, Sharma M, Südhof TC
Proceedings of the National Academy of Sciences of the United States of America (2014) 11140: E4274-83. 111 011 WB
Phosphorylation of syntaxin 3B by CaMKII regulates the formation of t-SNARE complexes.
Liu X, Heidelberger R, Janz R
Molecular and cellular neurosciences (2014) 60: 53-62. 111 011 WB; tested species: mouse
C-terminal complexin sequence is selectively required for clamping and priming but not for Ca2+ triggering of synaptic exocytosis.
Kaeser-Woo YJ, Yang X, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 328: 2877-85. 111 011 WB; tested species: rat
CSPα promotes SNARE-complex assembly by chaperoning SNAP-25 during synaptic activity.
Sharma M, Burré J, Südhof TC
Nature cell biology (2011) 131: 30-9. 111 011 WB; tested species: mouse
SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 111 011 WB, ICC
SV2 mediates entry of tetanus neurotoxin into central neurons.
Yeh FL, Dong M, Yao J, Tepp WH, Lin G, Johnson EA, Chapman ER
PLoS pathogens (2010) 611: e1001207. 111 011 WB; tested species: mouse
SV2B regulates synaptotagmin 1 by direct interaction.
Lazzell DR, Belizaire R, Thakur P, Sherry DM, Janz R
The Journal of biological chemistry (2004) 27950: 52124-31. 111 011 WB; tested species: mouse
Regulation of releasable vesicle pool sizes by protein kinase A-dependent phosphorylation of SNAP-25.
Nagy G, Reim K, Matti U, Brose N, Binz T, Rettig J, Neher E, Sørensen JB
Neuron (2004) 413: 417-29. 111 011 WB
An essential role for vesicular glutamate transporter 1 (VGLUT1) in postnatal development and control of quantal size.
Wojcik SM, Rhee JS, Herzog E, Sigler A, Jahn R, Takamori S, Brose N, Rosenmund C
Proceedings of the National Academy of Sciences of the United States of America (2004) 10118: 7158-63. 111 011 WB; tested species: mouse
Synaptic biomarkers in the cerebrospinal fluid associate differentially with classical neuronal biomarkers in patients with Alzheimer's disease and frontotemporal dementia.
Das S, Goossens J, Jacobs D, Dewit N, Pijnenburg YAL, In 't Veld SGJG, Teunissen CE, Vanmechelen E
Alzheimer's research & therapy (2023) 151: 62. 111 011 IP; tested species: human
Aggregation of mutant cysteine string protein-α via Fe-S cluster binding is mitigated by iron chelators.
Naseri NN, Ergel B, Kharel P, Na Y, Huang Q, Huang R, Dolzhanskaya N, Burré J, Velinov MT, Sharma M
Nature structural & molecular biology (2020) 272: 192-201. 111 011 WB, IP; tested species: mouse
Rapid and Sensitive Nano-Immunosensors for Botulinum.
Cheng HP, Chuang HS
ACS sensors (2019) : . 111 011 IP; tested species: human
CaV2.2 Gates Calcium-Independent but Voltage-Dependent Secretion in Mammalian Sensory Neurons.
Chai Z, Wang C, Huang R, Wang Y, Zhang X, Wu Q, Wang Y, Wu X, Zheng L, Zhang C, Guo W, et al.
Neuron (2017) 966: 1317-1326.e4. 111 011 WB, IP, ICC; tested species: rat
α-Synuclein induced cholesterol lowering increases tonic and reduces depolarization-evoked synaptic vesicle recycling and glutamate release.
Lazarevic V, Yang Y, Paslawski W, Svenningsson P
NPJ Parkinson's disease (2022) 81: 71. 111 011 ICC; tested species: mouse
Synaptotagmin-7-mediated activation of spontaneous NMDAR currents is disrupted in bipolar disorder susceptibility variants.
Wang QW, Wang YH, Wang B, Chen Y, Lu SY, Yao J
PLoS biology (2021) 197: e3001323. 111 011 ICC; tested species: mouse
The phosphoprotein Synapsin Ia regulates the kinetics of dense-core vesicle release.
Yang HJ, Chen PC, Huang CT, Cheng TL, Hsu SP, Chen CY, Lu JC, Wang CT
The Journal of neuroscience : the official journal of the Society for Neuroscience (2021) : . 111 011 WB, ICC; tested species: rat
Endophilin-A coordinates priming and fusion of neurosecretory vesicles via intersectin.
Gowrisankaran S, Houy S, Del Castillo JGP, Steubler V, Gelker M, Kroll J, Pinheiro PS, Schwitters D, Halbsgut N, Pechstein A, van Weering JRT, et al.
Nature communications (2020) 111: 1266. 111 011 WB, ICC; tested species: mouse
Intersectin-Mediated Clearance of SNARE Complexes Is Required for Fast Neurotransmission.
Jäpel M, Gerth F, Sakaba T, Bacetic J, Yao L, Koo SJ, Maritzen T, Freund C, Haucke V
Cell reports (2020) 302: 409-420.e6. 111 011 ICC; tested species: mouse
Riluzole attenuates the efficacy of glutamatergic transmission by interfering with the size of the readily releasable neurotransmitter pool.
Lazarevic V, Yang Y, Ivanova D, Fejtova A, Svenningsson P
Neuropharmacology (2018) : . 111 011 ICC; tested species: rat
CaV2.2 Gates Calcium-Independent but Voltage-Dependent Secretion in Mammalian Sensory Neurons.
Chai Z, Wang C, Huang R, Wang Y, Zhang X, Wu Q, Wang Y, Wu X, Zheng L, Zhang C, Guo W, et al.
Neuron (2017) 966: 1317-1326.e4. 111 011 WB, IP, ICC; tested species: rat
Atoxic derivative of botulinum neurotoxin A as a prototype molecular vehicle for targeted delivery to the neuronal cytoplasm.
Vazquez-Cintron EJ, Vakulenko M, Band PA, Stanker LH, Johnson EA, Ichtchenko K
PloS one (2014) 91: e85517. 111 011 ICC; tested species: rat
Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins.
Wilhelm BG, Mandad S, Truckenbrodt S, Kröhnert K, Schäfer C, Rammner B, Koo SJ, Claßen GA, Krauss M, Haucke V, Urlaub H, et al.
Science (New York, N.Y.) (2014) 3446187: 1023-8. 111 011 ICC, WB; tested species: rat
Synaptotagmin interaction with SNAP-25 governs vesicle docking, priming, and fusion triggering.
Mohrmann R, de Wit H, Connell E, Pinheiro PS, Leese C, Bruns D, Davletov B, Verhage M, Sørensen JB
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3336: 14417-30. 111 011 ICC
SV31 is a Zn2+-binding synaptic vesicle protein.
Barth J, Zimmermann H, Volknandt W
Journal of neurochemistry (2011) 1184: 558-70. 111 011 WB, ICC
Temporal characteristics of vesicular fusion in astrocytes: examination of synaptobrevin 2-laden vesicles at single vesicle resolution.
Malarkey EB, Parpura V
The Journal of physiology (2011) 58917: 4271-300. 111 011 ICC; tested species: rat
Nanobodies reveal an extra-synaptic population of SNAP-25 and Syntaxin 1A in hippocampal neurons.
Maidorn M, Olichon A, Rizzoli SO, Opazo F
mAbs () 112: 305-321. 111 011 ICC; tested species: rat
Hemisynapse Formation Between Target Astrocytes and Cortical Neuron Axons in vitro.
Teng Z, Gottmann K
Frontiers in molecular neuroscience (2022) 15: 829506. 111 011 IHC; tested species: mouse
The Isolated Mouse Jejunal Afferent Nerve Assay as a Tool to Assess the Effect of Botulinum Neurotoxins in Visceral Nociception.
Retailleau K, Martin V, Lezmi S, Nicoleau C, Maignel J
Toxins (2022) 143: . 111 011 IHC; tested species: mouse
Recombinant botulinum neurotoxin serotype A1 in vivo characterization.
Périer C, Martin V, Cornet S, Favre-Guilmard C, Rocher MN, Bindler J, Wagner S, Andriambeloson E, Rudkin B, Marty R, Vignaud A, et al.
Pharmacology research & perspectives (2021) 95: e00857. 111 011 IHC; tested species: mouse
A High-Resolution Method for Quantitative Molecular Analysis of Functionally Characterized Individual Synapses.
Holderith N, Heredi J, Kis V, Nusser Z
Cell reports (2020) 324: 107968. 111 011 IHC; tested species: rat
Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells.
Kirizs T, Kerti-Szigeti K, Lorincz A, Nusser Z
The European journal of neuroscience (2014) 3911: 1771-83. 111 011 EM; tested species: rat
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 111 011
WB
ICC
IHC-P
Background

SNAP 25 (synaptosome-associated protein of 25 kDa) is a highly conserved protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP 25 is the target of Botulinum neurotoxin A and E which cleave off 9 and 26 amino acids, respectively, from the C-terminus.
SNAP 25 is part of the exocytotic fusion complex (v-SNARE) of neurons where it assembles with syntaxin 1 and synaptobrevin. It is abundantly localized on the neuronal plasmalemma and on recycling vesicles including synaptic vesicles. It is also expressed in neuroendocrine cells. There are two splice-variants, SNAP 25A and 25B.
Other products for this target