SNAP25 antibody - 111 008 K.O.

SNAP25 is a palmitoylated plasma membrane protein; required for exocytosis

Rabbit monoclonal recombinant IgG

Cat. No.: 111 008

Amount: 50 µg

Price: $415.00

Cat. No. 111 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery IP: yes (see remarks) ICC: 1 : 1000 gallery IHC: not tested yet IHC-P: 1 : 200 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb71.1
Subtype	IgG1 (κ light chain)
Immunogen	Full-length recombinant rat SNAP25B protein (UniProt Id: P60881-1)
Epitop	AA 20 to 40 from rat SNAP25B (UniProt Id: P60881-1)
Reactivity	Reacts with: human (P60880), rat (P60881-1), mouse (P60879), vertebrates, invertebrates, zebrafish. Other species not tested yet.
Specificity	Detects both splice variants SNAP 25A and B. Recognizes the Botulinum neurotoxin A and E cleavage products. K.O. validated
Remarks	This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 71.1. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. IP: Immunoprecipitation not quantitative, appears to depend on the binding status of the protein.
Data sheet	111_008.pdf

Cat. No.: 111 008

Amount: 50 µg

Price: $415.00

ICC

IHC-P

Background

SNAP25 (synaptosome-associated protein of 25 kD) is a highly conserved protein anchored to the cytosolic face of membranes via palmitoyl side chains in the middle of the molecule. SNAP25 is the target of Botulinum neurotoxins A and E which cleave off 9 and 26 amino acids, respectively, from the C-terminus.
SNAP25 is part of the exocytotic fusion complex (v-SNARE) of neurons where it assembles with syntaxin1 and synaptobrevin. It is abundantly localized on the neuronal plasmalemma and on recycling vesicles including synaptic vesicles. It is also expressed in neuroendocrine cells. There are two splice-variants, SNAP25A and 25B.

1 ) Lin RC et al., Annu. Rev. Cell Dev. Biol. (2000)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Jahn R et al., Annu. Rev. Biochem. (1999)
4 ) Fasshauer D et al., J. Biol. Chem. (1997)
5 ) Südhof TC et al., Nature (1995)
6 ) Blasi J et al., Nature (1993)
7 ) Prescott GR et al., BMC Neurosci (2011)
8 ) Schulze KL et al., Cell (1995)

Protocols

Standard protocol

ICC

IHC-P

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