Synaptic Systems - Syntaxin1B

Cat. No.: 110 403

Amount: 50 µg

Price: $455.00

Cat. No. 110 403	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 100 up to 1 : 1000 gallery IHC: 1 : 1000 gallery IHC-P: 1 : 500 gallery ELISA: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Immunogen	Synthetic peptide corresponding to AA 171 to 187 from rat Syntaxin1B (UniProt Id: P61265)
Reactivity	Reacts with: human (P61266), rat (P61265), mouse (P61264), hamster, cow, pig, chicken, zebrafish. Other species not tested yet.
Specificity	Specific for syntaxin 1B, no cross reactivity to syntaxin 1A. K.O. validated PubMed: 32572454
Matching control protein/peptide	110-1BP
Remarks	ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 110 011 as capture antibodies. The ELISA-protocol for membrane proteins is recommended.
Data sheet	110_403.pdf

References for Syntaxin1B - 110 403

The proteomic landscape of synaptic diversity across brain regions and cell types.
van Oostrum M, Blok TM, Giandomenico SL, Tom Dieck S, Tushev G, Fürst N, Langer JD, Schuman EM
Cell (2023) 18624: 5411-5427.e23. 110 403 WB; tested species: mouse

Epilepsy-causing STX1B mutations translate altered protein functions into distinct phenotypes in mouse neurons.
Vardar G, Gerth F, Schmitt XJ, Rautenstrauch P, Trimbuch T, Schubert J, Lerche H, Rosenmund C, Freund C
Brain : a journal of neurology (2020) : . 110 403 WB, ICC; KO verified; tested species: mouse

Changes in synaptic proteins precede neurodegeneration markers in preclinical Alzheimer's disease cerebrospinal fluid.
Lleó A, Núñez-Llaves R, Alcolea D, Chiva C, Balateu-Paños D, Colom-Cadena M, Gomez-Giro G, Muñoz L, Querol-Vilaseca M, Pegueroles J, Rami L, et al.
Molecular & cellular proteomics : MCP (2019) : . 110 403 WB; tested species: human

The neural cell adhesion molecule promotes maturation of the presynaptic endocytotic machinery by switching synaptic vesicle recycling from adaptor protein 3 (AP-3)- to AP-2-dependent mechanisms.
Shetty A, Sytnyk V, Leshchyns'ka I, Puchkov D, Haucke V, Schachner M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3342: 16828-45. 110 403 WB

Titration of Syntaxin1 in mammalian synapses reveals multiple roles in vesicle docking, priming, and release probability.
Arancillo M, Min SW, Gerber S, Münster-Wandowski A, Wu YJ, Herman M, Trimbuch T, Rah JC, Ahnert-Hilger G, Riedel D, Südhof TC, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3342: 16698-714. 110 403 WB, ICC

Point mutation in syntaxin-1A causes abnormal vesicle recycling, behaviors, and short term plasticity.
Watanabe Y, Katayama N, Takeuchi K, Togano T, Itoh R, Sato M, Yamazaki M, Abe M, Sato T, Oda K, Yokoyama M, et al.
The Journal of biological chemistry (2013) 28848: 34906-19. 110 403 WB; tested species: mouse

Cat. No.: 110 403

Amount: 50 µg

Price: $455.00

ICC

IHC

IHC-P

Background

Syntaxin 1, also known as p35, is a small integral membrane protein that is abundantly expressed in neurons and neuroendocrine cells. It was initially discovered as HPC-1. Syntaxin 1 is an essential component of the exocytotic fusion machine and interacts with several other proteins important for synaptic function, including its partners in the fusion complex synaptobrevin, SNAP 25, α-SNAP, synaptotagmin 1, Munc 18/n-Sec1 and Ca²⁺-channels.
Syntaxin 1 is localized primarily to the neuronal plasmalemma and is concentrated in synapses where pools of the protein are also present on recycling organelles including synaptic vesicles. It is the main target of one of the Botulinum neurotoxins BoNT/C1 which, however, cannot cleave the protein when complexed with its partner proteins in the fusion complex.

1 ) Lin RC et al., Annu. Rev. Cell Dev. Biol. (2000)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Foletti DL et al., J. Neurosci. (2000)
4 ) Jahn R et al., Annu. Rev. Biochem. (1999)
5 ) Südhof TC et al., Nature (1995)
6 ) Bennett MK et al., Science (1992)
7 ) Inoue A et al., J. Biol. Chem. (1992)
8 ) Hirai Y et al., Cell (1992)
9 ) Barnstable CJ et al., Brain Res. (1985)

Protocols

Standard protocol

ICC

IHC

IHC-P

ELISA

Membrane proteins

OTHER

Preabsorbtion

Other products for this target

Syntaxin1B antibody - 110 403 K.O.

References for Syntaxin1B - 110 403