Synaptic Systems - Syntaxin1B

Cat. No.: 110 402

Amount: 200 µl

Price: $355.00

Cat. No. 110 402	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 100 up to 1 : 1000 gallery IHC: 1 : 1000 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 171 to 187 from rat Syntaxin1B (UniProt Id: P61265)
Reactivity	Reacts with: human (P61266), rat (P61265), mouse (P61264), hamster, cow, pig, chicken, zebrafish. Other species not tested yet.
Specificity	Specific for syntaxin 1B, no cross reactivity to syntaxin 1A. K.O. validated PubMed: 26203110
Matching control protein/peptide	110-1BP
Data sheet	110_402.pdf

References for Syntaxin1B - 110 402

Analysis of the neuromuscular deficits caused by STAM1 deficiency.
McLean JW, VanHart M, McWilliams MP, Farmer CB, Crossman DK, Cowell RM, Wilson JA, Wilson SM
Current research in neurobiology (2024) 7: 100138. 110 402 WB; tested species: mouse

Palmitoylation is required for Sept8-204 and Sept5 to form vesicle-like structure and colocalize with synaptophysin.
Liu H, Tan R, Tong J, Wen S, Wu C, Rao M, Zhu J, Qi S, Kong E
Journal of cellular biochemistry (2024) : . 110 402 WB; tested species: mouse

Syntabulin regulates neuronal excitation/inhibition balance and epileptic seizures by transporting syntaxin 1B.
Ke P, Gu J, Liu J, Liu Y, Tian X, Ma Y, Meng Y, Xiao F
Cell death discovery (2023) 91: 187. 110 402 WB, IP, ICC; tested species: mouse

Reduced synaptic proteins and SNARE complexes in Down syndrome with Alzheimer's disease and the Dp16 mouse Down syndrome model: Impact of APP gene dose.
Chen XQ, Zuo X, Becker A, Head E, Mobley WC
Alzheimer's & dementia : the journal of the Alzheimer's Association (2022) : . 110 402 WB; tested species: human,mouse

Pulse-Chase Proteomics of the App Knockin Mouse Models of Alzheimer's Disease Reveals that Synaptic Dysfunction Originates in Presynaptic Terminals.
Hark TJ, Rao NR, Castillon C, Basta T, Smukowski S, Bao H, Upadhyay A, Bomba-Warczak E, Nomura T, O'Toole ET, Morgan GP, et al.
Cell systems (2020) : . 110 402 WB, IHC; tested species: mouse

Tuning of glutamate, but not GABA, release by an intra-synaptic vesicles APP domain whose function can be modulated by β- or α-secretase cleavage.
Yao W, Tambini MD, Liu X, D'Adamio L
The Journal of neuroscience : the official journal of the Society for Neuroscience (2019) : . 110 402 WB; tested species: mouse

Syntaxin 1B is important for mouse postnatal survival and proper synaptic function at the mouse neuromuscular junctions.
Wu YJ, Tejero R, Arancillo M, Vardar G, Korotkova T, Kintscher M, Schmitz D, Ponomarenko A, Tabares L, Rosenmund C
Journal of neurophysiology (2015) 1144: 2404-17. 110 402 WB, IHC; KO verified

Quantitative Proteomic Analysis Reveals Molecular Adaptations in the Hippocampal Synaptic Active Zone of Chronic Mild Stress-Unsusceptible Rats.
Zhou J, Liu Z, Yu J, Han X, Fan S, Shao W, Chen J, Qiao R, Xie P
The international journal of neuropsychopharmacology (2015) 191: . 110 402 WB

Stapling of the botulinum type A protease to growth factors and neuropeptides allows selective targeting of neuroendocrine cells.
Arsenault J, Ferrari E, Niranjan D, Cuijpers SA, Gu C, Vallis Y, O'Brien J, Davletov B
Journal of neurochemistry (2013) 1262: 223-33. 110 402 WB

Syntaxin 1B suppresses macropinocytosis and semaphorin 3A-induced growth cone collapse.
Kabayama H, Takeuchi M, Taniguchi M, Tokushige N, Kozaki S, Mizutani A, Nakamura T, Mikoshiba K
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3120: 7357-64. 110 402 WB

How pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane.
Tsai PS, Garcia-Gil N, van Haeften T, Gadella BM
PloS one (2010) 56: e11204. 110 402 WB, IP

Vesicle associated membrane protein (VAMP)-7 and VAMP-8, but not VAMP-2 or VAMP-3, are required for activation-induced degranulation of mature human mast cells.
Sander LE, Frank SP, Bolat S, Blank U, Galli T, Bigalke H, Bischoff SC, Lorentz A
European journal of immunology (2008) 383: 855-63. 110 402 WB; tested species: human

Retinal pigment epithelial cells exhibit unique expression and localization of plasma membrane syntaxins which may contribute to their trafficking phenotype.
Low SH, Marmorstein LY, Miura M, Li X, Kudo N, Marmorstein AD, Weimbs T
Journal of cell science (2002) 115Pt 23: 4545-53. 110 402 WB, IHC

Co-immunoprecipitation with Tau Isoform-specific Antibodies Reveals Distinct Protein Interactions and Highlights a Putative Role for 2N Tau in Disease.
Liu C, Song X, Nisbet R, Götz J
The Journal of biological chemistry (2016) 29115: 8173-88. 110 402 IP

How pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane.
Tsai PS, Garcia-Gil N, van Haeften T, Gadella BM
PloS one (2010) 56: e11204. 110 402 WB, IP

SNAP23 deficiency causes severe brain dysplasia through the loss of radial glial cell polarity.
Kunii M, Noguchi Y, Yoshimura SI, Kanda S, Iwano T, Avriyanti E, Atik N, Sato T, Sato K, Ogawa M, Harada A, et al.
The Journal of cell biology (2021) 2201: . 110 402 ICC, IHC; KD verified; tested species: mouse

Dynamin controls neuropeptide secretion by organizing dense-core vesicle fusion sites.
Moro A, van Nifterick A, Toonen RF, Verhage M
Science advances (2021) 721: . 110 402 ICC; tested species: mouse

Novel cell types, neurosecretory cells, and body plan of the early-diverging metazoan Trichoplax adhaerens.
Smith CL, Varoqueaux F, Kittelmann M, Azzam RN, Cooper B, Winters CA, Eitel M, Fasshauer D, Reese TS
Current biology : CB (2014) 2414: 1565-1572. 110 402 ICC

Rare autism-associated variants implicate syntaxin 1 (STX1 R26Q) phosphorylation and the dopamine transporter (hDAT R51W) in dopamine neurotransmission and behaviors.
Cartier E, Hamilton PJ, Belovich AN, Shekar A, Campbell NG, Saunders C, Andreassen TF, Gether U, Veenstra-Vanderweele J, Sutcliffe JS, Ulery-Reynolds PG, et al.
EBioMedicine (2015) 22: 135-146. 110 402 IHC; tested species: mouse

Changes in retinal synaptic proteins in the transgenic model expressing a mutant HRG4 (UNC119).
Kubota S, Kobayashi A, Mori N, Higashide T, McLaren MJ, Inana G
Investigative ophthalmology & visual science (2002) 432: 308-13. 110 402 IHC

Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission.
Vasileva M, Horstmann H, Geumann C, Gitler D, Kuner T
The European journal of neuroscience (2012) 368: 3005-20. 110 402 ELISA

Cat. No.: 110 402

Amount: 200 µl

Price: $355.00

How pig sperm prepares to fertilize: stable acrosome docking to the plasma membrane.
Tsai PS, Garcia-Gil N, van Haeften T, Gadella BM
PloS one (2010) 56: e11204. 110 402 WB, IP

ICC

IHC

IHC-P

Background

Syntaxin 1, also known as p35, is a small integral membrane protein that is abundantly expressed in neurons and neuroendocrine cells. It was initially discovered as HPC-1. Syntaxin 1 is an essential component of the exocytotic fusion machine and interacts with several other proteins important for synaptic function, including its partners in the fusion complex synaptobrevin, SNAP 25, α-SNAP, synaptotagmin 1, Munc 18/n-Sec1 and Ca²⁺-channels.
Syntaxin 1 is localized primarily to the neuronal plasmalemma and is concentrated in synapses where pools of the protein are also present on recycling organelles including synaptic vesicles. It is the main target of one of the Botulinum neurotoxins BoNT/C1 which, however, cannot cleave the protein when complexed with its partner proteins in the fusion complex.

1 ) Lin RC et al., Annu. Rev. Cell Dev. Biol. (2000)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Foletti DL et al., J. Neurosci. (2000)
4 ) Jahn R et al., Annu. Rev. Biochem. (1999)
5 ) Südhof TC et al., Nature (1995)
6 ) Bennett MK et al., Science (1992)
7 ) Inoue A et al., J. Biol. Chem. (1992)
8 ) Hirai Y et al., Cell (1992)
9 ) Barnstable CJ et al., Brain Res. (1985)

Protocols

Standard protocol

ICC

IHC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Syntaxin1B antibody - 110 402 K.O.

References for Syntaxin1B - 110 402