Tailor-made Antibodies
and Tools for Life Science

Syntaxin1A antibody - 110 302 K.O.

Syntaxin1 is a plasma membrane SNARE protein essential for synaptic vesicle fusion
Rabbit polyclonal antiserum
Cat. No.: 110 302
Amount: 200 µl
Price: $360.00
Cat. No. 110 302 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: 1 : 100 gallery  
IHC: not tested yet
IHC-P: not tested yet
ELISA: yes (see remarks)
Immunogen Synthetic peptide corresponding to AA 172 to 189 from rat Syntaxin1A (UniProt Id: P32851)
Reactivity Reacts with: human (Q16623), rat (P32851), mouse (O35526), cow.
No signal: zebrafish.
Other species not tested yet.
Specificity Specific for syntaxin 1A, no cross reactivity to syntaxin 1B. K.O. validated PubMed: 28031464
Remarks

ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 110 011 as capture antibodies. The ELISA-protocol for membrane proteins is recommended.

Data sheet 110_302.pdf

References for Syntaxin1A - 110 302

Disrupting stroke-induced GAT-1-syntaxin1A interaction promotes functional recovery after stroke.
Lin YH, Wu F, Li TY, Lin L, Gao F, Zhu LJ, Xu XM, Chen MY, Hou YL, Zhang CJ, Wu HY, et al.
Cell reports. Medicine (2024) 511: 101789. 110 302 WB; tested species: mouse
Analysis of the neuromuscular deficits caused by STAM1 deficiency.
McLean JW, VanHart M, McWilliams MP, Farmer CB, Crossman DK, Cowell RM, Wilson JA, Wilson SM
Current research in neurobiology (2024) 7: 100138. 110 302 WB; tested species: mouse
Syntabulin regulates neuronal excitation/inhibition balance and epileptic seizures by transporting syntaxin 1B.
Ke P, Gu J, Liu J, Liu Y, Tian X, Ma Y, Meng Y, Xiao F
Cell death discovery (2023) 91: 187. 110 302 WB, IP; tested species: mouse
A novel synaptopathy-defective synaptic vesicle protein trafficking in the mutant CHMP2B mouse model of frontotemporal dementia.
Clayton EL, Bonnycastle K, Isaacs AM, Cousin MA, Schorge S
Journal of neurochemistry (2022) 1603: 412-425. 110 302 WB; tested species: mouse
Plasma membrane flipping of Syntaxin-2 regulates its inhibitory action on insulin granule exocytosis.
Kang F, Xie L, Qin T, Miao Y, Kang Y, Takahashi T, Liang T, Xie H, Gaisano HY
Nature communications (2022) 131: 6512. 110 302 IP, WB; tested species: human,mouse
Phospholipase D1 Ablation Disrupts Mouse Longitudinal Hippocampal Axis Organization and Functioning.
Santa-Marinha L, Castanho I, Silva RR, Bravo FV, Miranda AM, Meira T, Morais-Ribeiro R, Marques F, Xu Y, Point du Jour K, Wenk M, et al.
Cell reports (2020) 3012: 4197-4208.e6. 110 302 WB; tested species: mouse
Intersectin-Mediated Clearance of SNARE Complexes Is Required for Fast Neurotransmission.
Jäpel M, Gerth F, Sakaba T, Bacetic J, Yao L, Koo SJ, Maritzen T, Freund C, Haucke V
Cell reports (2020) 302: 409-420.e6. 110 302 WB; tested species: mouse
Actions of Rab27B-GTPase on mammalian central excitatory synaptic transmission.
Arias-Hervert ER, Xu N, Njus M, Murphy GG, Hou Y, Williams JA, Lentz SI, Ernst SA, Stuenkel EL
Physiological reports (2020) 89: e14428. 110 302 WB; tested species: mouse
VAMP4 maintains a Ca2+-sensitive pool of spontaneously recycling synaptic vesicles.
Lin PY, Chanaday NL, Horvath PM, Ramirez DMO, Monteggia LM, Kavalali ET
The Journal of neuroscience : the official journal of the Society for Neuroscience (2020) : . 110 302 WB; tested species: rat
New Roles of Syntaxin-1A in Insulin Granule Exocytosis and Replenishment.
Liang T, Qin T, Xie L, Dolai S, Zhu D, Prentice KJ, Wheeler M, Kang Y, Osborne L, Gaisano HY
The Journal of biological chemistry (2017) 2926: 2203-2216. 110 302 WB, IHC; KO verified; tested species: mouse
PRRT2 Is a Key Component of the Ca(2+)-Dependent Neurotransmitter Release Machinery.
Valente P, Castroflorio E, Rossi P, Fadda M, Sterlini B, Cervigni RI, Prestigio C, Giovedì S, Onofri F, Mura E, Guarnieri FC, et al.
Cell reports (2016) 151: 117-31. 110 302 WB
Point mutation in syntaxin-1A causes abnormal vesicle recycling, behaviors, and short term plasticity.
Watanabe Y, Katayama N, Takeuchi K, Togano T, Itoh R, Sato M, Yamazaki M, Abe M, Sato T, Oda K, Yokoyama M, et al.
The Journal of biological chemistry (2013) 28848: 34906-19. 110 302 WB, IP; tested species: mouse
Munc18-1 controls SNARE protein complex assembly during human sperm acrosomal exocytosis.
Rodríguez F, Zanetti MN, Mayorga LS, Tomes CN
The Journal of biological chemistry (2012) 28752: 43825-39. 110 302 WB, ICC; tested species: human
Vesicle associated membrane protein (VAMP)-7 and VAMP-8, but not VAMP-2 or VAMP-3, are required for activation-induced degranulation of mature human mast cells.
Sander LE, Frank SP, Bolat S, Blank U, Galli T, Bigalke H, Bischoff SC, Lorentz A
European journal of immunology (2008) 383: 855-63. 110 302 WB; tested species: human,rat
Ectopic expression of syntaxin 1 in the ER redirects TI-VAMP- and cellubrevin-containing vesicles.
Martinez-Arca S, Proux-Gillardeaux V, Alberts P, Louvard D, Galli T
Journal of cell science (2003) 116Pt 13: 2805-16. 110 302 WB, ICC
Cat. No.: 110 302
Amount: 200 µl
Price: $360.00