Tailor-made Antibodies
and Tools for Live Science
Home|||||||Technical Support|

Rab 3d antibody - 107 303

Rab3 proteins are the major Rab isoforms in brain. They are highly enriched on synaptic vesicles
Rabbit polyclonal purified antibody
Cat. No.: 107 303
Amount: 50 µg
Price: $370.00
Cat. No. 107 303 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: yes
ICC: not tested yet
IHC: not tested yet
IHC-P: 1 : 200 gallery  
Immunogen Synthetic peptide corresponding to AA 3 to 16 and 193 to 210 from rat Rab3d (UniProt Id: Q63942)
Reactivity Reacts with: rat (Q63942), mouse (P35276).
Other species not tested yet.
Specificity Specific for Rab 3d, no cross reactivity to other Rab 3 proteins.
Remarks

This antibody has been purified over a full-length Rab 3d column.

Data sheet 107_303.pdf

References for Rab 3d - 107 303

Intestinal goblet cells sample and deliver lumenal antigens by regulated endocytic uptake and transcytosis.
Gustafsson JK, Davis JE, Rappai T, McDonald KG, Kulkarni DH, Knoop KA, Hogan SP, Fitzpatrick JA, Lencer WI, Newberry RD
eLife (2021) 10: . 107 303 IHC; tested species: mouse
Cat. No.: 107 303
Amount: 50 µg
Price: $370.00
Intestinal goblet cells sample and deliver lumenal antigens by regulated endocytic uptake and transcytosis.
Gustafsson JK, Davis JE, Rappai T, McDonald KG, Kulkarni DH, Knoop KA, Hogan SP, Fitzpatrick JA, Lencer WI, Newberry RD
eLife (2021) 10: . 107 303 IHC; tested species: mouse
Background

Rab 3 is a member of the Rab protein family that belongs to the ras-related superfamily of small monomeric GTPases. Four related isoforms of Rab 3 are known (Rab 3a, 3b, 3c, and 3d). Rab 3a and 3c are predominantly expressed in neurons and neuroendocrine cells where they are localized to synaptic vesicles. Unlike the integral membrane proteins of synaptic vesicles, Rab 3a/c is absent from the Golgi complex and thus does not result in immunostaining of the axo-dendritic region as sometimes seen with e.g. synaptophysin, synaptobrevin/VAMP, or synaptogyrin.
Rab 3b and 3d are expressed in non-neuronal tissues such as adipocytes and the exocrine pancreas (3d). It has been shown that overexpression of Rab 3 inhibits Ca2+ regulated excocytosis and converts it into an constitutive Ca2+ independent exocytosis mechanism.