Synaptic Systems

Cat. No.: 107 011

Amount: 100 µg

Price: $415.00

Cat. No. 107 011	100 µg purified IgG, lyophilized. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not recommended ICC: 1 : 100 up to 1 : 500 gallery IHC: yes IHC-P: 1 : 200 up to 1 : 500 gallery EM: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.
Clone	42.1
Subtype	IgG1 (κ light chain)
Immunogen	Full length rat recombinant Rab3a (UniProt Id: P63012)
Epitop	AA 95 to 151 from rat Rab3a (UniProt Id: P63012)
Reactivity	Reacts with: human (P20336, P20337, Q96E17, O95716), rat (P63012, Q63941, P62824, Q63942), mouse (P63011, Q9CZT8, P62823, P35276), vertebrates. Other species not tested yet.
Specificity	Specific for all four Rab 3 isoforms a, b, c d.
Data sheet	107_011.pdf

References for Rab3 - 107 011

Monitoring of activity-driven trafficking of endogenous synaptic proteins through proximity labeling.
Pascual-Caro C, de Juan-Sanz J
PLoS biology (2024) 2210: e3002860. 107 011 WB; tested species: rat

cAMP-EPAC-PKCε-RIM1α signaling regulates presynaptic long-term potentiation and motor learning.
Wang XT, Zhou L, Dong BB, Xu FX, Wang DJ, Shen EW, Cai XY, Wang Y, Wang N, Ji SJ, Chen W, et al.
eLife (2023) 12: . 107 011 WB; tested species: mouse

Selective endocytosis of Ca2+-permeable AMPARs by the Alzheimer's disease risk factor CALM bidirectionally controls synaptic plasticity.
Azarnia Tehran D, Kochlamazashvili G, Pampaloni NP, Sposini S, Shergill JK, Lehmann M, Pashkova N, Schmidt C, Löwe D, Napieczynska H, Heuser A, et al.
Science advances (2022) 821: eabl5032. 107 011 WB; tested species: mouse

A presynaptic phosphosignaling hub for lasting homeostatic plasticity.
Müller JA, Betzin J, Santos-Tejedor J, Mayer A, Oprişoreanu AM, Engholm-Keller K, Paulußen I, Gulakova P, McGovern TD, Gschossman LJ, Schönhense E, et al.
Cell reports (2022) 393: 110696. 107 011 WB; tested species: mouse

JIP3 localises to exocytic vesicles and focal adhesions in the growth cones of differentiated PC12 cells.
Caswell PT, Dickens M
Molecular and cellular biochemistry (2017) : . 107 011 WB, ICC; tested species: rat

Parkinson Sac Domain Mutation in Synaptojanin 1 Impairs Clathrin Uncoating at Synapses and Triggers Dystrophic Changes in Dopaminergic Axons.
Cao M, Wu Y, Ashrafi G, McCartney AJ, Wheeler H, Bushong EA, Boassa D, Ellisman MH, Ryan TA, De Camilli P
Neuron (2017) 934: 882-896.e5. 107 011 WB; tested species: mouse

Dynamic Partitioning of Synaptic Vesicle Pools by the SNARE-Binding Protein Tomosyn.
Cazares VA, Njus MM, Manly A, Saldate JJ, Subramani A, Ben-Simon Y, Sutton MA, Ashery U, Stuenkel EL
The Journal of neuroscience : the official journal of the Society for Neuroscience (2016) 3644: 11208-11222. 107 011 WB; tested species: rat

Ubiquitin-Synaptobrevin Fusion Protein Causes Degeneration of Presynaptic Motor Terminals in Mice.
Liu Y, Li H, Sugiura Y, Han W, Gallardo G, Khvotchev M, Zhang Y, Kavalali ET, Südhof TC, Lin W
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3533: 11514-31. 107 011 WB

Synaptotagmin-12 phosphorylation by cAMP-dependent protein kinase is essential for hippocampal mossy fiber LTP.
Kaeser-Woo YJ, Younts TJ, Yang X, Zhou P, Wu D, Castillo PE, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3323: 9769-80. 107 011 WB

Rab3 proteins involved in vesicle biogenesis and priming in embryonic mouse chromaffin cells.
Schonn JS, van Weering JR, Mohrmann R, Schlüter OM, Südhof TC, de Wit H, Verhage M, Sørensen JB
Traffic (Copenhagen, Denmark) (2010) 1111: 1415-28. 107 011 WB, ICC

Doc2b is a high-affinity Ca2+ sensor for spontaneous neurotransmitter release.
Groffen AJ, Martens S, Díez Arazola R, Cornelisse LN, Lozovaya N, de Jong AP, Goriounova NA, Habets RL, Takai Y, Borst JG, Brose N, et al.
Science (New York, N.Y.) (2010) 3275973: 1614-8. 107 011 WB; tested species: mouse

Synaptic and vesicular co-localization of the glutamate transporters VGLUT1 and VGLUT2 in the mouse hippocampus.
Herzog E, Takamori S, Jahn R, Brose N, Wojcik SM
Journal of neurochemistry (2006) 993: 1011-8. 107 011 WB; tested species: mouse

Large scale protein identification in intracellular aquaporin-2 vesicles from renal inner medullary collecting duct.
Barile M, Pisitkun T, Yu MJ, Chou CL, Verbalis MJ, Shen RF, Knepper MA
Molecular & cellular proteomics : MCP (2005) 48: 1095-106. 107 011 WB

Synaptic targeting of rabphilin-3A, a synaptic vesicle Ca2+/phospholipid-binding protein, depends on rab3A/3C.
Li C, Takei K, Geppert M, Daniell L, Stenius K, Chapman ER, Jahn R, De Camilli P, Südhof TC
Neuron (1994) 134: 885-98. 107 011 WB, IHC

Structure of the murine rab3A gene: correlation of genomic organization with antibody epitopes.
Baumert M, Fischer von Mollard G, Jahn R, Südhof TC
The Biochemical journal (1993) 293 ( Pt 1): 157-63. 107 011 WB

Association of Rab3A with synaptic vesicles at late stages of the secretory pathway.
Matteoli M, Takei K, Cameron R, Hurlbut P, Johnston PA, Südhof TC, Jahn R, De Camilli P
The Journal of cell biology (1991) 1153: 625-33. 107 011 ICC, WB; tested species: rat

A Super-Resolved View of the Alzheimer's Disease-Related Amyloidogenic Pathway in Hippocampal Neurons.
Yu Y, Gao Y, Winblad B, Tjernberg LO, Schedin-Weiss S
Journal of Alzheimer's disease : JAD (2021) 832: 833-852. 107 011 ICC; tested species: mouse

Effect of zinc binding residues in growth hormone (GH) and altered intracellular zinc content on regulated GH secretion.
Petkovic V, Miletta MC, Eblé A, Iliev DI, Binder G, Flück CE, Mullis PE
Endocrinology (2013) 15411: 4215-25. 107 011 ICC; tested species: rat

Intracellular distribution of amyloid beta peptide and its relationship to the lysosomal system.
Zheng L, Cedazo-Minguez A, Hallbeck M, Jerhammar F, Marcusson J, Terman A
Translational neurodegeneration (2012) 11: 19. 107 011 ICC; tested species: human

Mutations in the LGI1/Epitempin gene on 10q24 cause autosomal dominant lateral temporal epilepsy.
Morante-Redolat JM, Gorostidi-Pagola A, Piquer-Sirerol S, Sáenz A, Poza JJ, Galán J, Gesk S, Sarafidou T, Mautner VF, Binelli S, Staub E, et al.
Human molecular genetics (2002) 119: 1119-28. 107 011 ICC

The zebrafish pinball wizard gene encodes WRB, a tail-anchored-protein receptor essential for inner-ear hair cells and retinal photoreceptors.
Lin SY, Vollrath MA, Mangosing S, Shen J, Cardenas E, Corey DP
The Journal of physiology (2016) 5944: 895-914. 107 011 IHC; tested species: zebrafish

Rabconnectin3α promotes stable activity of the H+ pump on synaptic vesicles in hair cells.
Einhorn Z, Trapani JG, Liu Q, Nicolson T
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 3232: 11144-56. 107 011 IHC; tested species: zebrafish

Impaired activity-dependent plasticity of quantal amplitude at the neuromuscular junction of Rab3A deletion and Rab3A earlybird mutant mice.
Wang X, Wang Q, Yang S, Bucan M, Rich MM, Engisch KL
The Journal of neuroscience : the official journal of the Society for Neuroscience (2011) 3110: 3580-8. 107 011 IHC

Microtubule-dependent transport of secretory vesicles in RBL-2H3 cells.
Smith AJ, Pfeiffer JR, Zhang J, Martinez AM, Griffiths GM, Wilson BS
Traffic (Copenhagen, Denmark) (2003) 45: 302-12. 107 011 EM; tested species: rat

Cat. No.: 107 011

Amount: 100 µg

Price: $415.00

ICC

IHC-P

Background

Rab 3 is a member of the Rab protein family that belongs to the ras-related superfamily of small monomeric GTPases. Four related isoforms of Rab 3 are known (Rab 3a, 3b, 3c, and 3d). Rab 3a and 3c are predominantly expressed in neurons and neuroendocrine cells where they are localized to synaptic vesicles. Unlike the integral membrane proteins of synaptic vesicles, Rab 3a/c is absent from the Golgi complex and thus does not result in immunostaining of the axo-dendritic region as sometimes seen with e.g. synaptophysin, synaptobrevin/VAMP, or synaptogyrin.
Rab 3b and 3d are expressed in non-neuronal tissues such as adipocytes and the exocrine pancreas (3d). It has been shown that overexpression of Rab 3 inhibits Ca²⁺ regulated excocytosis and converts it into an constitutive Ca²⁺ independent exocytosis mechanism.

1 ) Geppert M et al., Annu. Rev. Neurosci. (1998)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Geppert M et al., Nature (1997)
4 ) Südhof TC et al., Nature (1995)
5 ) Fischer von Mollard G et al., Eur. J. Cell Biol. (1994)
6 ) Chen X et al., J. Biol. Chem. (2003)
7 ) Millar AL et al., Histol. Histopathol. (2002)
8 ) Pavlos NJ et al., J. Mol. Endocrinol. (2001)
9 ) Lin CG et al., Biochem. J. (1997)
10 ) Fischer von Mollard G et al., J. Biol. Chem. (1994)

Protocols

ICC

IHC

IHC-P

Other products for this target

Rab3 antibody - 107 011

References for Rab3 - 107 011