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Home > Products > 106 011C3

Synapsin1 antibody - 106 011C3 K.O.

Synapsins are peripheral synaptic vesicle proteins and substrate for several protein kinases
Mouse monoclonal purified IgG
Cat. No.: 106 011C3
Amount: 100 µg
Price: $475.00
Cat. No. 106 011C3 100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 3.

Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy.

Cyanine 2: λex 492 nm / λem 508 nm
Sulfo-Cyanine 3: λex 554 nm / λem 568 nm
Sulfo-Cyanine 5: λex 649 nm / λem 667 nm

(Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy.
Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates.

 Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 100 up to 1 : 2000 gallery  
IHC: 1 : 100 up to 1 : 500 gallery  
IHC-P: not tested yet
Label Sulfo-Cyanine 3
Clone 46.1
Subtype IgG1
Immunogen full-length recombinant rat Synapsin1 (UniProt Id: P09951)
Epitop AA 435 to 475 from rat Synapsin1 (UniProt Id: P09951)
Reactivity Reacts with: human (P17600), rat (P09951), mouse (O88935), mammals.
Weaker signal: zebrafish, chicken, other vertebrates.
Other species not tested yet.
Specificity Specific for synapsin 1a and 1b independent of phosphorylation state. K.O. validated
Data sheet 106_011c3.pdf

References for Synapsin1 - 106 011C3

Neuronal differentiation requires BRAT1 complex to remove REST from chromatin.
Dokaneheifard S, Gomes Dos Santos H, Guiselle Valencia M, Arigela H, Edupuganti RR, Shiekhattar R
Proceedings of the National Academy of Sciences of the United States of America (2024) 12123: e2318740121. 106 011C3 ICC; tested species: human
Inflammatory Molecules Released by Mechanically Injured Astrocytes Trigger Presynaptic Loss in Cortical Neuronal Networks.
Lantoine J, Procès A, Villers A, Halliez S, Buée L, Ris L, Gabriele S
ACS chemical neuroscience (2021) 1220: 3885-3897. 106 011C3 ICC; tested species: rat
Cat. No.: 106 011C3
Amount: 100 µg
Price: $475.00
Neuronal differentiation requires BRAT1 complex to remove REST from chromatin.
Dokaneheifard S, Gomes Dos Santos H, Guiselle Valencia M, Arigela H, Edupuganti RR, Shiekhattar R
Proceedings of the National Academy of Sciences of the United States of America (2024) 12123: e2318740121. 106 011C3 ICC; tested species: human
Inflammatory Molecules Released by Mechanically Injured Astrocytes Trigger Presynaptic Loss in Cortical Neuronal Networks.
Lantoine J, Procès A, Villers A, Halliez S, Buée L, Ris L, Gabriele S
ACS chemical neuroscience (2021) 1220: 3885-3897. 106 011C3 ICC; tested species: rat
ICC
IHC
Background

Synapsins are neuron-specific phosphoproteins that are exclusively associated with small synaptic vesicles, with little or no expression in other tissues including neuroendocrine cells. In mammals, three distinct synapsin genes (synapsin1, 2, and 3) encode more than eight neuronal isoforms.
Synapsin1 is one of the most specific markers of synapses throughout the central and peripheral nervous system. In addition to synaptic nerve terminals, the protein is also present in certain sensory nerve endings. It is expressed in two splice variants (synapsin 1a and synapsin 1b). Synapsin1 interacts with vesicle membranes as well as with actin and spectrin.
Synapsin2 is expressed in the nervous system and also two splice variants were described so far, while synapsin3 shows a more restricted expression pattern and is mainly found in the hypocampus.
Synapsins are major phosphoproteins and are substrates for several protein kinases such as PKA, CaMK I and CaMK II. Synapsin1 is widely used as reference substrate for calmodulin-dependent protein kinases.
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