Synaptic Systems - Synapsin1

Cat. No.: 106 009

Amount: 50 µg

Price: $415.00

WB

ICC

IHC

IHC-P

Background

Synapsins are neuron-specific phosphoproteins that are exclusively associated with small synaptic vesicles, with little or no expression in other tissues including neuroendocrine cells. In mammals, three distinct synapsin genes (synapsin1, 2, and 3) encode more than eight neuronal isoforms.
Synapsin1 is one of the most specific markers of synapses throughout the central and peripheral nervous system. In addition to synaptic nerve terminals, the protein is also present in certain sensory nerve endings. It is expressed in two splice variants (synapsin 1a and synapsin 1b). Synapsin1 interacts with vesicle membranes as well as with actin and spectrin.
Synapsin2 is expressed in the nervous system and also two splice variants were described so far, while synapsin3 shows a more restricted expression pattern and is mainly found in the hypocampus.
Synapsins are major phosphoproteins and are substrates for several protein kinases such as PKA, CaMK I and CaMK II. Synapsin1 is widely used as reference substrate for calmodulin-dependent protein kinases.

1 ) Hosaka M et al., Neuron (1999)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Rosahl TW et al., Nature (1995)
4 ) Südhof TC et al., Nature (1995)

Protocols

WB

ICC

IHC

IHC-P

Other products for this target

Cat. No. 106 009	50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 4000 (AP-staining) gallery IP: not tested yet ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: 1 : 2000 up to 1 : 4000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Ch46.1
Subtype	IgY (λ light chain)
Immunogen	full-length recombinant rat Synapsin1 (UniProt Id: P09951)
Epitop	AA 435 to 475 from rat Synapsin1 (UniProt Id: P09951)
Reactivity	Reacts with: human (P17600), rat (P09951), mouse (O88935), other mammals. Weaker signal: zebrafish, chicken, other vertebrates. Other species not tested yet.
Specificity	Specific for synapsin 1a and 1b independent of phosphorylation state. K.O. validated
Remarks	This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 46.1. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. Therefore, the antibody can be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	106_009.pdf

Synapsin1 antibody - 106 009 K.O.