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Home > Products > 105 311CpH

Synaptotagmin1 antibody luminal domain - 105 311CpH

Synaptotagmin1 is a Ca2+-sensor on synaptic vesicles that triggers neurotransmitter release

pH-sensitive probe fluorescent only under acidic conditions

Mouse monoclonal purified IgG
Cat. No.: 105 311CpH
Amount: 100 µg
Price: $465.00
Cat. No. 105 311CpH 100 µg purified IgG, lyophilized, fluorescence-labeled with CypHer5E.

CypHer5E® is a pH-sensitive dye, fluorescent only after antibody internalization at the acidic pH of the lumen of the synaptic vesicle. CypHer5E® can be detected with the filter sets for Cy5®.

CypHer5E®: λex 647 nm / λem 667 nm

This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Application Number US 2006-0051758 and related patents. Cy, CyDye, and CypHer are trademarks of GE-Healthcare Ltd. Purchase of reagents related to the CypHer technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from GE-Healthcare Bio-Science Corp., 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA.

 Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 50 up to 1 : 300 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet
EM: N/A
ELISA: N/A
FACS: not tested yet
Label CypHer5E
Clone 604.2
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity Reacts with: rat (P21707).
No signal: mouse (P46096), zebrafish.
Other species not tested yet.
Remarks

ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures. The pH sensitive dye regaines its fluorescence after the reacidification of the synaptic vesicle lumen.
Data sheet 105_311cph.pdf

References for Synaptotagmin1 - 105 311CpH

Transient Confinement of CaV2.1 Ca2+-Channel Splice Variants Shapes Synaptic Short-Term Plasticity.
Heck J, Parutto P, Ciuraszkiewicz A, Bikbaev A, Freund R, Mitlöhner J, Alonso M, Fejtova A, Holcman D, Heine M
Neuron (2019) : . 105 311CpH ICC; tested species: rat
Riluzole attenuates the efficacy of glutamatergic transmission by interfering with the size of the readily releasable neurotransmitter pool.
Lazarevic V, Yang Y, Ivanova D, Fejtova A, Svenningsson P
Neuropharmacology (2018) : . 105 311CpH ICC; tested species: rat
Regulated Dynamic Trafficking of Neurexins Inside and Outside of Synaptic Terminals.
Neupert C, Schneider R, Klatt O, Reissner C, Repetto D, Biermann B, Niesmann K, Missler M, Heine M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3540: 13629-47. 105 311CpH ICC
Dynamic properties of the alkaline vesicle population at hippocampal synapses.
Röther M, Brauner JM, Ebert K, Welzel O, Jung J, Bauereiss A, Kornhuber J, Groemer TW
PloS one (2014) 97: e102723. 105 311CpH ICC; tested species: rat
Synapse clusters are preferentially formed by synapses with large recycling pool sizes.
Welzel O, Tischbirek CH, Jung J, Kohler EM, Svetlitchny A, Henkel AW, Kornhuber J, Groemer TW
PloS one (2010) 510: e13514. 105 311CpH ICC
α-Synuclein induced cholesterol lowering increases tonic and reduces depolarization-evoked synaptic vesicle recycling and glutamate release.
Lazarevic V, Yang Y, Paslawski W, Svenningsson P
NPJ Parkinson's disease (2022) 81: 71. 105 311CpH UPTAKE; tested species: mouse
Rho-kinase inhibition by fasudil modulates pre-synaptic vesicle dynamics.
Saal KA, Warth Pérez Arias C, Roser AE, Christoph Koch J, Bähr M, Rizzoli SO, Lingor P
Journal of neurochemistry (2020) : . 105 311CpH UPTAKE; tested species: rat
CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles.
Ivanova D, Imig C, Camacho M, Reinhold A, Guhathakurta D, Montenegro-Venegas C, Cousin MA, Gundelfinger ED, Rosenmund C, Cooper B, Fejtova A, et al.
Cell reports (2020) 307: 2444-2459.e7. 105 311CpH UPTAKE; tested species: mouse
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 105 311CpH UPTAKE; tested species: rat
The pH probe CypHer™5E is effectively quenched by FM dyes.
Welzel O, Loy K, Tischbirek CH, Tabor A, Gmeiner P, Kornhuber J, Groemer TW
Journal of fluorescence (2013) 233: 487-94. 105 311CpH UPTAKE; tested species: rat
Blocking endocytosis enhances short-term synaptic depression under conditions of normal availability of vesicles.
Hua Y, Woehler A, Kahms M, Haucke V, Neher E, Klingauf J
Neuron (2013) 802: 343-9. 105 311CpH UPTAKE; tested species: rat
Key physiological parameters dictate triggering of activity-dependent bulk endocytosis in hippocampal synapses.
Wenzel EM, Morton A, Ebert K, Welzel O, Kornhuber J, Cousin MA, Groemer TW
PloS one (2012) 76: e38188. 105 311CpH UPTAKE
Independent vesicle pools underlie different modes of release during neuronal development.
Andreae LC, Fredj NB, Burrone J
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 325: 1867-74. 105 311CpH UPTAKE
Use-dependent inhibition of synaptic transmission by the secretion of intravesicularly accumulated antipsychotic drugs.
Tischbirek CH, Wenzel EM, Zheng F, Huth T, Amato D, Trapp S, Denker A, Welzel O, Lueke K, Svetlitchny A, Rauh M, et al.
Neuron (2012) 745: 830-44. 105 311CpH UPTAKE
A readily retrievable pool of synaptic vesicles.
Hua Y, Sinha R, Thiel CS, Schmidt R, Hüve J, Martens H, Hell SW, Egner A, Klingauf J
Nature neuroscience (2011) 147: 833-9. 105 311CpH UPTAKE
Amyloid precursor protein is trafficked and secreted via synaptic vesicles.
Groemer TW, Thiel CS, Holt M, Riedel D, Hua Y, Hüve J, Wilhelm BG, Klingauf J
PloS one (2011) 64: e18754. 105 311CpH UPTAKE
Systematic heterogeneity of fractional vesicle pool sizes and release rates of hippocampal synapses.
Welzel O, Henkel AW, Stroebel AM, Jung J, Tischbirek CH, Ebert K, Kornhuber J, Rizzoli SO, Groemer TW
Biophysical journal (2011) 1003: 593-601. 105 311CpH UPTAKE
A common origin of synaptic vesicles undergoing evoked and spontaneous fusion.
Hua Y, Sinha R, Martineau M, Kahms M, Klingauf J
Nature neuroscience (2010) 1312: 1451-3. 105 311CpH UPTAKE
Cat. No.: 105 311CpH
Amount: 100 µg
Price: $465.00
Transient Confinement of CaV2.1 Ca2+-Channel Splice Variants Shapes Synaptic Short-Term Plasticity.
Heck J, Parutto P, Ciuraszkiewicz A, Bikbaev A, Freund R, Mitlöhner J, Alonso M, Fejtova A, Holcman D, Heine M
Neuron (2019) : . 105 311CpH ICC; tested species: rat
Riluzole attenuates the efficacy of glutamatergic transmission by interfering with the size of the readily releasable neurotransmitter pool.
Lazarevic V, Yang Y, Ivanova D, Fejtova A, Svenningsson P
Neuropharmacology (2018) : . 105 311CpH ICC; tested species: rat
Regulated Dynamic Trafficking of Neurexins Inside and Outside of Synaptic Terminals.
Neupert C, Schneider R, Klatt O, Reissner C, Repetto D, Biermann B, Niesmann K, Missler M, Heine M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2015) 3540: 13629-47. 105 311CpH ICC
Dynamic properties of the alkaline vesicle population at hippocampal synapses.
Röther M, Brauner JM, Ebert K, Welzel O, Jung J, Bauereiss A, Kornhuber J, Groemer TW
PloS one (2014) 97: e102723. 105 311CpH ICC; tested species: rat
Synapse clusters are preferentially formed by synapses with large recycling pool sizes.
Welzel O, Tischbirek CH, Jung J, Kohler EM, Svetlitchny A, Henkel AW, Kornhuber J, Groemer TW
PloS one (2010) 510: e13514. 105 311CpH ICC
α-Synuclein induced cholesterol lowering increases tonic and reduces depolarization-evoked synaptic vesicle recycling and glutamate release.
Lazarevic V, Yang Y, Paslawski W, Svenningsson P
NPJ Parkinson's disease (2022) 81: 71. 105 311CpH UPTAKE; tested species: mouse
Rho-kinase inhibition by fasudil modulates pre-synaptic vesicle dynamics.
Saal KA, Warth Pérez Arias C, Roser AE, Christoph Koch J, Bähr M, Rizzoli SO, Lingor P
Journal of neurochemistry (2020) : . 105 311CpH UPTAKE; tested species: rat
CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles.
Ivanova D, Imig C, Camacho M, Reinhold A, Guhathakurta D, Montenegro-Venegas C, Cousin MA, Gundelfinger ED, Rosenmund C, Cooper B, Fejtova A, et al.
Cell reports (2020) 307: 2444-2459.e7. 105 311CpH UPTAKE; tested species: mouse
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 105 311CpH UPTAKE; tested species: rat
The pH probe CypHer™5E is effectively quenched by FM dyes.
Welzel O, Loy K, Tischbirek CH, Tabor A, Gmeiner P, Kornhuber J, Groemer TW
Journal of fluorescence (2013) 233: 487-94. 105 311CpH UPTAKE; tested species: rat
Blocking endocytosis enhances short-term synaptic depression under conditions of normal availability of vesicles.
Hua Y, Woehler A, Kahms M, Haucke V, Neher E, Klingauf J
Neuron (2013) 802: 343-9. 105 311CpH UPTAKE; tested species: rat
Key physiological parameters dictate triggering of activity-dependent bulk endocytosis in hippocampal synapses.
Wenzel EM, Morton A, Ebert K, Welzel O, Kornhuber J, Cousin MA, Groemer TW
PloS one (2012) 76: e38188. 105 311CpH UPTAKE
Independent vesicle pools underlie different modes of release during neuronal development.
Andreae LC, Fredj NB, Burrone J
The Journal of neuroscience : the official journal of the Society for Neuroscience (2012) 325: 1867-74. 105 311CpH UPTAKE
Use-dependent inhibition of synaptic transmission by the secretion of intravesicularly accumulated antipsychotic drugs.
Tischbirek CH, Wenzel EM, Zheng F, Huth T, Amato D, Trapp S, Denker A, Welzel O, Lueke K, Svetlitchny A, Rauh M, et al.
Neuron (2012) 745: 830-44. 105 311CpH UPTAKE
A readily retrievable pool of synaptic vesicles.
Hua Y, Sinha R, Thiel CS, Schmidt R, Hüve J, Martens H, Hell SW, Egner A, Klingauf J
Nature neuroscience (2011) 147: 833-9. 105 311CpH UPTAKE
Amyloid precursor protein is trafficked and secreted via synaptic vesicles.
Groemer TW, Thiel CS, Holt M, Riedel D, Hua Y, Hüve J, Wilhelm BG, Klingauf J
PloS one (2011) 64: e18754. 105 311CpH UPTAKE
Systematic heterogeneity of fractional vesicle pool sizes and release rates of hippocampal synapses.
Welzel O, Henkel AW, Stroebel AM, Jung J, Tischbirek CH, Ebert K, Kornhuber J, Rizzoli SO, Groemer TW
Biophysical journal (2011) 1003: 593-601. 105 311CpH UPTAKE
A common origin of synaptic vesicles undergoing evoked and spontaneous fusion.
Hua Y, Sinha R, Martineau M, Kahms M, Klingauf J
Nature neuroscience (2010) 1312: 1451-3. 105 311CpH UPTAKE
ICC
Background

Synaptotagmin1, also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.
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