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Home > Products > 105 311C5

Synaptotagmin1 antibody luminal domain - 105 311C5

Synaptotagmin1 is a Ca2+-sensor on synaptic vesicles that triggers neurotransmitter release
Mouse monoclonal purified IgG
Cat. No.: 105 311C5
Amount: 100 µg
Price: $465.00
Cat. No. 105 311C5 100 µg purified IgG, lyophilized, fluorescence-labeled with Cyanine 5.

Fluorescence labeled antibodies conjugated to (Sulfo-)Cyanine dyes are well suited for standard epi-fluorescence setups and confocal microscopy.

Cyanine 2: λex 492 nm / λem 508 nm
Sulfo-Cyanine 3: λex 554 nm / λem 568 nm
Sulfo-Cyanine 5: λex 649 nm / λem 667 nm

(Sulfo-)Cyanine 5 dyes are well suited for STORM high resolution microscopy.
Sulfo-Cyanines are highly water soluble and allow a higher labeling degree and consequently brighter conjugates.
Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 50 up to 1 : 300 (see remarks) gallery  
IHC: not tested yet
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label Sulfo-Cyanine 5
Clone 604.2
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity Reacts with: rat (P21707).
No signal: mouse (P46096), zebrafish.
Other species not tested yet.
Remarks

ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.
Data sheet 105_311c5.pdf

References for Synaptotagmin1 - 105 311C5

Chaperone-mediated autophagy in neuronal dendrites utilizes activity-dependent lysosomal exocytosis for protein disposal.
Grochowska KM, Sperveslage M, Raman R, Failla AV, Głów D, Schulze C, Laprell L, Fehse B, Kreutz MR
Cell reports (2023) 428: 112998. 105 311C5 ICC; tested species: rat
A CRHR1 antagonist prevents synaptic loss and memory deficits in a trauma-induced delirium-like syndrome.
Cursano S, Battaglia CR, Urrutia-Ruiz C, Grabrucker S, Schön M, Bockmann J, Braumüller S, Radermacher P, Roselli F, Huber-Lang M, Boeckers TM, et al.
Molecular psychiatry (2020) : . 105 311C5 UPTAKE; tested species: mouse
GABA(A) receptors can initiate the formation of functional inhibitory GABAergic synapses.
Fuchs C, Abitbol K, Burden JJ, Mercer A, Brown L, Iball J, Anne Stephenson F, Thomson AM, Jovanovic JN
The European journal of neuroscience (2013) 388: 3146-58. 105 311C5 UPTAKE; tested species: rat
Cat. No.: 105 311C5
Amount: 100 µg
Price: $465.00
Chaperone-mediated autophagy in neuronal dendrites utilizes activity-dependent lysosomal exocytosis for protein disposal.
Grochowska KM, Sperveslage M, Raman R, Failla AV, Głów D, Schulze C, Laprell L, Fehse B, Kreutz MR
Cell reports (2023) 428: 112998. 105 311C5 ICC; tested species: rat
A CRHR1 antagonist prevents synaptic loss and memory deficits in a trauma-induced delirium-like syndrome.
Cursano S, Battaglia CR, Urrutia-Ruiz C, Grabrucker S, Schön M, Bockmann J, Braumüller S, Radermacher P, Roselli F, Huber-Lang M, Boeckers TM, et al.
Molecular psychiatry (2020) : . 105 311C5 UPTAKE; tested species: mouse
GABA(A) receptors can initiate the formation of functional inhibitory GABAergic synapses.
Fuchs C, Abitbol K, Burden JJ, Mercer A, Brown L, Iball J, Anne Stephenson F, Thomson AM, Jovanovic JN
The European journal of neuroscience (2013) 388: 3146-58. 105 311C5 UPTAKE; tested species: rat
ICC
Background

Synaptotagmin1, also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.
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