Synaptotagmin1 antibody luminal domain - 105 311BT

Synaptotagmin1 is a Ca²⁺-sensor on synaptic vesicles that triggers neurotransmitter release

Mouse monoclonal purified IgG

Cat. No.: 105 311BT

Amount: 100 µg

Price: $465.00

Cat. No. 105 311BT	100 µg purified IgG, lyophilized, labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known. Albumin was added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 gallery IP: yes ICC: 1 : 50 up to 1 : 300 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	biotin
Clone	604.2
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity	Reacts with: rat (P21707). No signal: mouse (P46096), zebrafish. Other species not tested yet.
Remarks	ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.
Data sheet	105_311bt.pdf

References for Synaptotagmin1 - 105 311BT

Unique dynamics and exocytosis properties of GABAergic synaptic vesicles revealed by three-dimensional single vesicle tracking.
Park C, Chen X, Tian CL, Park GN, Chenouard N, Lee H, Yeo XY, Jung S, Tsien RW, Bi GQ, Park H, et al.
Proceedings of the National Academy of Sciences of the United States of America (2021) 1189: . 105 311BT UPTAKE; tested species: rat

Real-time three-dimensional tracking of single synaptic vesicles reveals that synaptic vesicles undergoing kiss-and-run fusion remain close to their original fusion site before reuse.
Qin X, Tsien RW, Park H
Biochemical and biophysical research communications (2019) : . 105 311BT UPTAKE; tested species: rat

Cat. No.: 105 311BT

Amount: 100 µg

Price: $465.00

ICC

Background

Synaptotagmin1, also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca²⁺-binding C2-domains.
Ca²⁺-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca²⁺-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.

1 ) Geppert M et al., Annu. Rev. Neurosci. (1998)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Südhof TC et al., Nature (1995)
4 ) Geppert M et al., Cell (1994)
5 ) Brose N et al., Science (1992)
6 ) Perin MS et al., Nature (1990)

Protocols

Standard protocol

ICC

Other products for this target