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Synaptotagmin 1 lumenal domain - 105 311

Ca2+-sensor on synaptic vesicles, triggers neurotransmitter release
Monoclonal mouse purified IgG
Cat. No.: 105 311
Amount: 100 µg
Price: 415.00 €
Cat. No. 105 311 100 µg purified IgG, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 gallery  
IP: yes
ICC: 1 : 50 up to 1 : 300 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: not tested yet
Clone 604.2
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 1 to 12 from rat Synaptotagmin1 (UniProt Id: P21707)
Epitop Epitop: AA 1 to 12 from rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity Reacts with: rat (P21707).
No signal: mouse (P46096), zebrafish.
Other species not tested yet.
Specificity Specific for rat synaptotagmin 1, no cross-reactivity to other synaptotagmins.
Remarks This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.
Data sheet 105_311.pdf

References for Synaptotagmin 1 - 105 311

Calcium-dependent interaction of the cytoplasmic region of synaptotagmin with membranes. Autonomous function of a single C2-homologous domain.
Chapman ER, Jahn R
The Journal of biological chemistry (1994) 2698: 5735-41. 105 311 WB; tested species: rat
VAP-SCRN1 interaction regulates dynamic endoplasmic reticulum remodeling and presynaptic function.
Lindhout FW, Cao Y, Kevenaar JT, Bodzęta A, Stucchi R, Boumpoutsari MM, Katrukha EA, Altelaar M, MacGillavry HD, Hoogenraad CC
The EMBO journal (2019) : e101345. 105 311 ICC, UPTAKE; tested species: rat
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 105 311 ICC, UPTAKE; tested species: rat
Alternative Splicing of P/Q-Type Ca2+ Channels Shapes Presynaptic Plasticity.
Thalhammer A, Contestabile A, Ermolyuk YS, Ng T, Volynski KE, Soong TW, Goda Y, Cingolani LA
Cell reports (2017) 202: 333-343. 105 311 ICC, UPTAKE; tested species: rat
Antibody-driven capture of synaptic vesicle proteins on the plasma membrane enables the analysis of their interactions with other synaptic proteins.
Richter KN, Patzelt C, Phan NTN, Rizzoli SO
Scientific reports (2019) 91: 9231. 105 311 UPTAKE; tested species: rat
VAP-SCRN1 interaction regulates dynamic endoplasmic reticulum remodeling and presynaptic function.
Lindhout FW, Cao Y, Kevenaar JT, Bodzęta A, Stucchi R, Boumpoutsari MM, Katrukha EA, Altelaar M, MacGillavry HD, Hoogenraad CC
The EMBO journal (2019) : e101345. 105 311 ICC, UPTAKE; tested species: rat
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 105 311 ICC, UPTAKE; tested species: rat
An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins.
Riemann D, Petkova A, Dresbach T, Wallrafen R
Journal of visualized experiments : JoVE (2018) 136: . 105 311 UPTAKE; tested species: rat
Alternative Splicing of P/Q-Type Ca2+ Channels Shapes Presynaptic Plasticity.
Thalhammer A, Contestabile A, Ermolyuk YS, Ng T, Volynski KE, Soong TW, Goda Y, Cingolani LA
Cell reports (2017) 202: 333-343. 105 311 ICC, UPTAKE; tested species: rat
Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis.
Martineau M, Somasundaram A, Grimm JB, Gruber TD, Choquet D, Taraska JW, Lavis LD, Perrais D
Nature communications (2017) 81: 1412. 105 311 UPTAKE; tested species: rat
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 105 311
Cat. No.: 105 311
Quantity: 100 µg
Price: 415.00 €
Calcium-dependent interaction of the cytoplasmic region of synaptotagmin with membranes. Autonomous function of a single C2-homologous domain.
Chapman ER, Jahn R
The Journal of biological chemistry (1994) 2698: 5735-41. 105 311 WB; tested species: rat
VAP-SCRN1 interaction regulates dynamic endoplasmic reticulum remodeling and presynaptic function.
Lindhout FW, Cao Y, Kevenaar JT, Bodzęta A, Stucchi R, Boumpoutsari MM, Katrukha EA, Altelaar M, MacGillavry HD, Hoogenraad CC
The EMBO journal (2019) : e101345. 105 311 ICC, UPTAKE; tested species: rat
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 105 311 ICC, UPTAKE; tested species: rat
Alternative Splicing of P/Q-Type Ca2+ Channels Shapes Presynaptic Plasticity.
Thalhammer A, Contestabile A, Ermolyuk YS, Ng T, Volynski KE, Soong TW, Goda Y, Cingolani LA
Cell reports (2017) 202: 333-343. 105 311 ICC, UPTAKE; tested species: rat
Antibody-driven capture of synaptic vesicle proteins on the plasma membrane enables the analysis of their interactions with other synaptic proteins.
Richter KN, Patzelt C, Phan NTN, Rizzoli SO
Scientific reports (2019) 91: 9231. 105 311 UPTAKE; tested species: rat
VAP-SCRN1 interaction regulates dynamic endoplasmic reticulum remodeling and presynaptic function.
Lindhout FW, Cao Y, Kevenaar JT, Bodzęta A, Stucchi R, Boumpoutsari MM, Katrukha EA, Altelaar M, MacGillavry HD, Hoogenraad CC
The EMBO journal (2019) : e101345. 105 311 ICC, UPTAKE; tested species: rat
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 105 311 ICC, UPTAKE; tested species: rat
An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins.
Riemann D, Petkova A, Dresbach T, Wallrafen R
Journal of visualized experiments : JoVE (2018) 136: . 105 311 UPTAKE; tested species: rat
Alternative Splicing of P/Q-Type Ca2+ Channels Shapes Presynaptic Plasticity.
Thalhammer A, Contestabile A, Ermolyuk YS, Ng T, Volynski KE, Soong TW, Goda Y, Cingolani LA
Cell reports (2017) 202: 333-343. 105 311 ICC, UPTAKE; tested species: rat
Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis.
Martineau M, Somasundaram A, Grimm JB, Gruber TD, Choquet D, Taraska JW, Lavis LD, Perrais D
Nature communications (2017) 81: 1412. 105 311 UPTAKE; tested species: rat
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 105 311
Background
Synaptotagmin 1 also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.