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Synaptotagmin 1 antibody luminal domain - 105 311

Synaptotagmin1 is a Ca2+-sensor on synaptic vesicles that triggers neurotransmitter release
Mouse monoclonal purified IgG
Cat. No.: 105 311
Amount: 100 µg
Price: $415.00
Cat. No. 105 311 100 µg purified IgG, lyophilized. Albumin was added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 1000 gallery  
IP: yes
ICC: 1 : 50 up to 1 : 300 (see remarks) gallery  
IHC: 1 : 500 gallery  
IHC-P: not tested yet
Clone 604.2
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 1 to 12 from rat Synaptotagmin1 (UniProt Id: P21707)
Epitop Epitop: AA 1 to 12 from rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity Reacts with: rat (P21707).
No signal: mouse (P46096), zebrafish.
Other species not tested yet.
Specificity Specific for rat synaptotagmin 1, no cross-reactivity to other synaptotagmins.
Remarks

ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.

Data sheet 105_311.pdf

References for Synaptotagmin 1 - 105 311

Calcium-dependent interaction of the cytoplasmic region of synaptotagmin with membranes. Autonomous function of a single C2-homologous domain.
Chapman ER, Jahn R
The Journal of biological chemistry (1994) 2698: 5735-41. 105 311 WB; tested species: rat
Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R.
Dankovich TM, Kaushik R, Olsthoorn LHM, Petersen GC, Giro PE, Kluever V, Agüi-Gonzalez P, Grewe K, Bao G, Beuermann S, Hadi HA, et al.
Nature communications (2021) 121: 7129. 105 311 IP; tested species: rat
Neuroligin-1 mediates presynaptic maturation through brain-derived neurotrophic factor signaling.
Petkova-Tuffy A, Gödecke N, Viotti J, Korte M, Dresbach T
BMC biology (2021) 191: 215. 105 311 ICC, UPTAKE; tested species: rat
Gold-Conjugated Nanobodies for Targeted Imaging Using High-Resolution Secondary Ion Mass Spectrometry.
Agüi-Gonzalez P, Dankovich TM, Rizzoli SO, Phan NTN
Nanomaterials (Basel, Switzerland) (2021) 117: . 105 311 ICC; tested species: rat
VAP-SCRN1 interaction regulates dynamic endoplasmic reticulum remodeling and presynaptic function.
Lindhout FW, Cao Y, Kevenaar JT, Bodzęta A, Stucchi R, Boumpoutsari MM, Katrukha EA, Altelaar M, MacGillavry HD, Hoogenraad CC
The EMBO journal (2019) : e101345. 105 311 ICC, UPTAKE; tested species: rat
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 105 311 ICC, UPTAKE; tested species: rat
Alternative Splicing of P/Q-Type Ca2+ Channels Shapes Presynaptic Plasticity.
Thalhammer A, Contestabile A, Ermolyuk YS, Ng T, Volynski KE, Soong TW, Goda Y, Cingolani LA
Cell reports (2017) 202: 333-343. 105 311 ICC, UPTAKE; tested species: rat
Neuroligin-1 mediates presynaptic maturation through brain-derived neurotrophic factor signaling.
Petkova-Tuffy A, Gödecke N, Viotti J, Korte M, Dresbach T
BMC biology (2021) 191: 215. 105 311 ICC, UPTAKE; tested species: rat
Differences in synaptic vesicle pool behavior between male and female hippocampal cultured neurons.
Sertel SM, Blumenstein W, Mandad S, Shomroni O, Salinas G, Rizzoli SO
Scientific reports (2021) 111: 17374. 105 311 UPTAKE; tested species: rat
CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles.
Ivanova D, Imig C, Camacho M, Reinhold A, Guhathakurta D, Montenegro-Venegas C, Cousin MA, Gundelfinger ED, Rosenmund C, Cooper B, Fejtova A, et al.
Cell reports (2020) 307: 2444-2459.e7. 105 311 UPTAKE; tested species: mouse
Antibody-driven capture of synaptic vesicle proteins on the plasma membrane enables the analysis of their interactions with other synaptic proteins.
Richter KN, Patzelt C, Phan NTN, Rizzoli SO
Scientific reports (2019) 91: 9231. 105 311 UPTAKE; tested species: rat
VAP-SCRN1 interaction regulates dynamic endoplasmic reticulum remodeling and presynaptic function.
Lindhout FW, Cao Y, Kevenaar JT, Bodzęta A, Stucchi R, Boumpoutsari MM, Katrukha EA, Altelaar M, MacGillavry HD, Hoogenraad CC
The EMBO journal (2019) : e101345. 105 311 ICC, UPTAKE; tested species: rat
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 105 311 ICC, UPTAKE; tested species: rat
An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins.
Riemann D, Petkova A, Dresbach T, Wallrafen R
Journal of visualized experiments : JoVE (2018) 136: . 105 311 UPTAKE; tested species: rat
Alternative Splicing of P/Q-Type Ca2+ Channels Shapes Presynaptic Plasticity.
Thalhammer A, Contestabile A, Ermolyuk YS, Ng T, Volynski KE, Soong TW, Goda Y, Cingolani LA
Cell reports (2017) 202: 333-343. 105 311 ICC, UPTAKE; tested species: rat
Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis.
Martineau M, Somasundaram A, Grimm JB, Gruber TD, Choquet D, Taraska JW, Lavis LD, Perrais D
Nature communications (2017) 81: 1412. 105 311 UPTAKE; tested species: rat
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 105 311
Cat. No.: 105 311
Amount: 100 µg
Price: $415.00
Calcium-dependent interaction of the cytoplasmic region of synaptotagmin with membranes. Autonomous function of a single C2-homologous domain.
Chapman ER, Jahn R
The Journal of biological chemistry (1994) 2698: 5735-41. 105 311 WB; tested species: rat
Extracellular matrix remodeling through endocytosis and resurfacing of Tenascin-R.
Dankovich TM, Kaushik R, Olsthoorn LHM, Petersen GC, Giro PE, Kluever V, Agüi-Gonzalez P, Grewe K, Bao G, Beuermann S, Hadi HA, et al.
Nature communications (2021) 121: 7129. 105 311 IP; tested species: rat
Neuroligin-1 mediates presynaptic maturation through brain-derived neurotrophic factor signaling.
Petkova-Tuffy A, Gödecke N, Viotti J, Korte M, Dresbach T
BMC biology (2021) 191: 215. 105 311 ICC, UPTAKE; tested species: rat
Gold-Conjugated Nanobodies for Targeted Imaging Using High-Resolution Secondary Ion Mass Spectrometry.
Agüi-Gonzalez P, Dankovich TM, Rizzoli SO, Phan NTN
Nanomaterials (Basel, Switzerland) (2021) 117: . 105 311 ICC; tested species: rat
VAP-SCRN1 interaction regulates dynamic endoplasmic reticulum remodeling and presynaptic function.
Lindhout FW, Cao Y, Kevenaar JT, Bodzęta A, Stucchi R, Boumpoutsari MM, Katrukha EA, Altelaar M, MacGillavry HD, Hoogenraad CC
The EMBO journal (2019) : e101345. 105 311 ICC, UPTAKE; tested species: rat
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 105 311 ICC, UPTAKE; tested species: rat
Alternative Splicing of P/Q-Type Ca2+ Channels Shapes Presynaptic Plasticity.
Thalhammer A, Contestabile A, Ermolyuk YS, Ng T, Volynski KE, Soong TW, Goda Y, Cingolani LA
Cell reports (2017) 202: 333-343. 105 311 ICC, UPTAKE; tested species: rat
Neuroligin-1 mediates presynaptic maturation through brain-derived neurotrophic factor signaling.
Petkova-Tuffy A, Gödecke N, Viotti J, Korte M, Dresbach T
BMC biology (2021) 191: 215. 105 311 ICC, UPTAKE; tested species: rat
Differences in synaptic vesicle pool behavior between male and female hippocampal cultured neurons.
Sertel SM, Blumenstein W, Mandad S, Shomroni O, Salinas G, Rizzoli SO
Scientific reports (2021) 111: 17374. 105 311 UPTAKE; tested species: rat
CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles.
Ivanova D, Imig C, Camacho M, Reinhold A, Guhathakurta D, Montenegro-Venegas C, Cousin MA, Gundelfinger ED, Rosenmund C, Cooper B, Fejtova A, et al.
Cell reports (2020) 307: 2444-2459.e7. 105 311 UPTAKE; tested species: mouse
Antibody-driven capture of synaptic vesicle proteins on the plasma membrane enables the analysis of their interactions with other synaptic proteins.
Richter KN, Patzelt C, Phan NTN, Rizzoli SO
Scientific reports (2019) 91: 9231. 105 311 UPTAKE; tested species: rat
VAP-SCRN1 interaction regulates dynamic endoplasmic reticulum remodeling and presynaptic function.
Lindhout FW, Cao Y, Kevenaar JT, Bodzęta A, Stucchi R, Boumpoutsari MM, Katrukha EA, Altelaar M, MacGillavry HD, Hoogenraad CC
The EMBO journal (2019) : e101345. 105 311 ICC, UPTAKE; tested species: rat
Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission.
Truckenbrodt S, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero EF, Rizzoli SO
The EMBO journal (2018) : . 105 311 ICC, UPTAKE; tested species: rat
An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins.
Riemann D, Petkova A, Dresbach T, Wallrafen R
Journal of visualized experiments : JoVE (2018) 136: . 105 311 UPTAKE; tested species: rat
Alternative Splicing of P/Q-Type Ca2+ Channels Shapes Presynaptic Plasticity.
Thalhammer A, Contestabile A, Ermolyuk YS, Ng T, Volynski KE, Soong TW, Goda Y, Cingolani LA
Cell reports (2017) 202: 333-343. 105 311 ICC, UPTAKE; tested species: rat
Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis.
Martineau M, Somasundaram A, Grimm JB, Gruber TD, Choquet D, Taraska JW, Lavis LD, Perrais D
Nature communications (2017) 81: 1412. 105 311 UPTAKE; tested species: rat
Endosomal sorting of readily releasable synaptic vesicles.
Hoopmann P, Punge A, Barysch SV, Westphal V, Bückers J, Opazo F, Bethani I, Lauterbach MA, Hell SW, Rizzoli SO
Proceedings of the National Academy of Sciences of the United States of America (2010) 10744: 19055-60. 105 311
Background
Synaptotagmin 1 also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.