Tailor-made Antibodies
and Tools for Live Science
Home|||||||Technical Support|

Synaptotagmin 1 antibody luminal domain - 105 308Ab635P

Synaptotagmin1 is a Ca2+-sensor on synaptic vesicles that triggers neurotransmitter release
Rabbit monoclonal recombinant IgG
Cat. No.: 105 308Ab635P
Amount: 50 µg
Price: $465.00
Cat. No. 105 308Ab635P 50 µg purified recombinant IgG, lyophilized, fluorescence-labeled with AbberiorStar 635P. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives.
Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications
 
WB: N/A
IP: N/A
ICC: 1 : 200 up to 1 : 500 gallery  
IHC: not tested yet
IHC-P: not tested yet
Label AbberiorStar 635P
Clone Rb604.2
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 1 to 12 from rat Synaptotagmin1 (UniProt Id: P21707)
Epitop Epitop: AA 1 to 12 from rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity Reacts with: rat (P21707).
No signal: mouse (P46096).
Other species not tested yet.
Specificity Specific for rat Synaptotagmin 1, no cross-reactivity to other Synaptotagmins.
Remarks

This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures.
The antibody is a chimeric antibody based on the monoclonal mouse antibody clone 604.2. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. The antibody has been expressed in mammalian cells.

Data sheet 105_308ab635p.pdf
Cat. No.: 105 308Ab635P
Amount: 50 µg
Price: $465.00
Background
Synaptotagmin 1 also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.
Protocols