Synaptotagmin 1 antibody luminal domain - 105 308Ab635P

Synaptotagmin1 is a Ca²⁺-sensor on synaptic vesicles that triggers neurotransmitter release

Rabbit monoclonal recombinant IgG

Cat. No.: 105 308Ab635P

Amount: 50 µg

Price: $465.00

Cat. No. 105 308Ab635P	50 µg purified recombinant IgG, lyophilized, fluorescence-labeled with AbberiorStar 635P. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: N/A IP: N/A ICC: 1 : 200 up to 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet
Label	AbberiorStar 635P
Clone	Rb604.2
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to AA 1 to 12 from rat Synaptotagmin1 (UniProt Id: P21707)
Epitop	Epitop: AA 1 to 12 from rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity	Reacts with: rat (P21707). No signal: mouse (P46096). Other species not tested yet.
Specificity	Specific for rat Synaptotagmin 1, no cross-reactivity to other Synaptotagmins.
Remarks	This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures. The antibody is a chimeric antibody based on the monoclonal mouse antibody clone 604.2. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. The antibody has been expressed in mammalian cells.
Data sheet	105_308ab635p.pdf

Cat. No.: 105 308Ab635P

Amount: 50 µg

Price: $465.00

ICC

Background

Synaptotagmin 1 also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca²⁺-binding C2-domains.
Ca²⁺-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca²⁺-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.

1 ) Geppert M, Annual review of neuroscience (1998) 21: 75-95.
2 ) Jahn R, Annual review of neuroscience (1994) 17: 219-46.
3 ) Südhof TC, Nature (1995) 3756533: 645-53.
4 ) Geppert M, Cell (1994) 794: 717-27.
5 ) Brose N, Science (New York, N.Y.) (1992) 2565059: 1021-5.

Protocols

ICC

PFA fixation

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