Synaptotagmin1 antibody luminal domain - 105 211 K.O.

Synaptotagmin1 is a Ca²⁺-sensor on synaptic vesicles that triggers neurotransmitter release

Mouse monoclonal purified IgG

Cat. No.: 105 211

Amount: 100 µg

Price: $415.00

Cat. No. 105 211	100 µg purified IgG, lyophilized. Albumin was added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP-staining ) (see remarks) gallery IP: yes ICC: 1 : 200 up to 1 : 500 (see remarks) gallery IHC: 1 : 500 (see remarks) IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	604.1
Subtype	IgG3 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity	Reacts with: rat (P21707), mouse (P46096). No signal: zebrafish. Other species not tested yet.
Specificity	K.O. validated
Remarks	WB: Only detects rat Synaptotagmin1 in Western blots. ICC: Antibody is suitable for the labeling of recycling vesicles of living rat and mouse neurons. It detects PFA fixed rat Synaptotagmin1, but is negative on PFA fixed mouse Synaptotagmin1. IHC: Antibody detects PFA fixed rat Synaptotagmin1, but is negative on PFA fixed mouse Synaptotagmin1.
Data sheet	105_211.pdf

Cat. No.: 105 211

Amount: 100 µg

Price: $415.00

ICC

Background

Synaptotagmin1, also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca²⁺-binding C2-domains.
Ca²⁺-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca²⁺-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.

1 ) Geppert M et al., Annu. Rev. Neurosci. (1998)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Südhof TC et al., Nature (1995)
4 ) Geppert M et al., Cell (1994)
5 ) Brose N et al., Science (1992)
6 ) Perin MS et al., Nature (1990)

Protocols

Standard protocol

ICC

IHC

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