Cat. No.: 105 101
Amount: 100 µl
Price:
$355.00
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| Cat. No. 105 101 | 100 µl ascites, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use. |
| Applications |
IP: yes ICC: yes (see remarks) IHC: not tested yet IHC-P: not tested yet |
| Clone | 604.4 |
| Subtype | IgG1 (κ light chain) |
| Epitop |
AA 1 to 12 from rat Synaptotagmin1 (UniProt Id: P21707) |
| Reactivity |
Reacts with: rat (P21707). Other species not tested yet. For unknown reasons antibodies raised against the luminal N-terminus of Synaptotagmin 1 show a strong preference for the rat protein. |
| Remarks |
ICC: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures. |
| Data sheet | 105_101.pdf |
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Synaptotagmin1, also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.