Synaptic Systems - Synaptotagmin1

Cat. No.: 105 015

Amount: 50 µg

Price: $460.00

Cat. No. 105 015	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: yes ICC: 1 : 500 (see remarks) gallery IHC: not tested yet IHC-P: 1 : 200 up to 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 405 to 421 from rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity	Reacts with: mouse (P46096), rat (P21707). Other species not tested yet.
Specificity	K.O. validated
Matching control protein/peptide	105-01P
Remarks	ICC: Methanol fixation is recommended.
Data sheet	105_015.pdf

References for Synaptotagmin1 - 105 015

Synaptotagmins 1 and 7 play complementary roles in somatodendritic dopamine release.
Hikima T, Witkovsky P, Khatri L, Chao M, Rice ME
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) : . 105 015 WB, IHC; tested species: mouse

Cat. No.: 105 015

Amount: 50 µg

Price: $460.00

ICC

IHC-P

Background

Synaptotagmin1, also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca²⁺-binding C2-domains.
Ca²⁺-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca²⁺-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.

1 ) Geppert M et al., Annu. Rev. Neurosci. (1998)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Südhof TC et al., Nature (1995)
4 ) Geppert M et al., Cell (1994)
5 ) Brose N et al., Science (1992)
6 ) Perin MS et al., Nature (1990)

Protocols

Standard protocol

ICC

IHC-P

OTHER

Preabsorbtion

Other products for this target

Synaptotagmin1 antibody cytoplasmic tail - 105 015 K.O.

References for Synaptotagmin1 - 105 015