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Synaptotagmin1 antibody cytoplasmic tail - 105 015 K.O.

Synaptotagmin1 is a Ca2+-sensor on synaptic vesicles that triggers neurotransmitter release
Guinea pig polyclonal purified antibody
Cat. No.: 105 015
Amount: 50 µg
Price: $460.00
Cat. No. 105 015 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: yes
ICC: 1 : 500 (see remarks) gallery  
IHC: not tested yet
IHC-P: 1 : 200 up to 1 : 500 gallery  
Immunogen Synthetic peptide corresponding to AA 405 to 421 from rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity Reacts with: mouse (P46096), rat (P21707).
Other species not tested yet.
Specificity K.O. validated
Matching control protein/peptide 105-01P
Remarks

ICC: Methanol fixation is recommended.

Data sheet 105_015.pdf

References for Synaptotagmin1 - 105 015

Synaptotagmins 1 and 7 play complementary roles in somatodendritic dopamine release.
Hikima T, Witkovsky P, Khatri L, Chao M, Rice ME
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) : . 105 015 WB, IHC; tested species: mouse
Synaptotagmins 1 and 7 play complementary roles in somatodendritic dopamine release.
Hikima T, Witkovsky P, Khatri L, Chao M, Rice ME
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) : . 105 015 WB, IHC; tested species: mouse
Cat. No.: 105 015
Amount: 50 µg
Price: $460.00
Synaptotagmins 1 and 7 play complementary roles in somatodendritic dopamine release.
Hikima T, Witkovsky P, Khatri L, Chao M, Rice ME
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) : . 105 015 WB, IHC; tested species: mouse
Synaptotagmins 1 and 7 play complementary roles in somatodendritic dopamine release.
Hikima T, Witkovsky P, Khatri L, Chao M, Rice ME
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) : . 105 015 WB, IHC; tested species: mouse
Background

Synaptotagmin1, also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.