Cat. No.: 105 015
Amount: 50 µg
Price:
$460.00
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| Cat. No. 105 015 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Immunogen | Synthetic peptide corresponding to AA 405 to 421 from rat Synaptotagmin1 (UniProt Id: P21707) |
| Reactivity |
Reacts with: mouse (P46096), rat (P21707). Other species not tested yet. |
| Specificity | K.O. validated |
| Matching control protein/peptide | 105-01P |
| Remarks |
ICC: Methanol fixation is recommended. |
| Data sheet | 105_015.pdf |
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Synaptotagmin1, also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.