Synaptotagmin1 antibody - 105 011AbRED K.O.

Synaptotagmin1 is a Ca²⁺-sensor on synaptic vesicles that triggers neurotransmitter release

Mouse monoclonal purified IgG

Cat. No.: 105 011AbRED

Amount: 100 µg

Price: $465.00

Cat. No. 105 011AbRED	100 µg purified IgG, lyophilized, fluorescence-labeled with abberior STAR RED. abberior STAR^® dyes have been especially developed for for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy. For many of the fluorescence labeled antibodies conjugated to abberior STAR^® dyes established fluorescence detection systems can be used. abberior STAR^® 635P: λ_ex 638 nm / λ_em 651 nm abberior STAR^® ORANGE: λ_ex 589 nm / λ_em 616 nm abberior STAR^® RED: λ_ex 638 nm / λ_em 655 nm This product or portions thereof is manufactured under license from abberior GmbH. abberior STAR is a trademark of abberior GmbH, Goettingen/Germany. Purchase of reagents related to the abberior STAR technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from abberior GmbH. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: N/A IP: N/A ICC: 1 : 300 (see remarks) gallery IHC: not tested yet IHC-P: not tested yet ELISA: N/A
Label	AbberiorStar RED
Clone	41.1
Subtype	IgG2a
Immunogen	Recombinant protein corresponding to AA 80 to 421 from rat Synaptotagmin1 (UniProt Id: P21707)
Epitop	Epitop: AA 150 to 240 from rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity	Reacts with: human (P21579), rat (P21707), mouse (P46096), mammals, zebrafish. Other species not tested yet.
Specificity	K.O. validated
Remarks	ICC: This antibody conjugate is especially suitable for high resolution STED microscopy.
Data sheet	105_011abred.pdf

Cat. No.: 105 011AbRED

Amount: 100 µg

Price: $465.00

ICC

Background

Synaptotagmin1, also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca²⁺-binding C2-domains.
Ca²⁺-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca²⁺-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.

1 ) Geppert M, Annual review of neuroscience (1998) 21: 75-95.
2 ) Jahn R, Annual review of neuroscience (1994) 17: 219-46.
3 ) Südhof TC, Nature (1995) 3756533: 645-53.
4 ) Geppert M, Cell (1994) 794: 717-27.
5 ) Brose N, Science (New York, N.Y.) (1992) 2565059: 1021-5.

Protocols

ICC

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