Synaptic Systems - Synaptotagmin1/2

Cat. No.: 105 002

Amount: 200 µl

Price: $355.00

Cat. No. 105 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: 1 : 400 gallery ELISA: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Immunogen	Synthetic peptide corresponding to AA 120 to 131 from rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity	Reacts with: human (P21579), rat (P21707), mouse (P46096), cow, chicken, goldfish, zebrafish. Other species not tested yet. [[105002-zebrafish-ihc.jpg]]
Specificity	Some cross-reactivity to synaptotagmin 2.
Matching control protein/peptide	105-0P
Remarks	ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 105 011 as capture antibodies. The ELISA-protocol for membrane proteins is recommended.
Data sheet	105_002.pdf

References for Synaptotagmin1/2 - 105 002

Suggestion of creatine as a new neurotransmitter by approaches ranging from chemical analysis and biochemistry to electrophysiology.
Bian X, Zhu J, Jia X, Liang W, Yu S, Li Z, Zhang W, Rao Y
eLife (2023) 12: . 105 002 WB; tested species: mouse

APC/CCdh1-Rock2 pathway controls dendritic integrity and memory.
Bobo-Jiménez V, Delgado-Esteban M, Angibaud J, Sánchez-Morán I, de la Fuente A, Yajeya J, Nägerl UV, Castillo J, Bolaños JP, Almeida A
Proceedings of the National Academy of Sciences of the United States of America (2017) 11417: 4513-4518. 105 002 WB

Membrane-tethered monomeric neurexin LNS-domain triggers synapse formation.
Gokce O, Südhof TC
The Journal of neuroscience : the official journal of the Society for Neuroscience (2013) 3336: 14617-28. 105 002 WB; tested species: mouse

Point mutation in syntaxin-1A causes abnormal vesicle recycling, behaviors, and short term plasticity.
Watanabe Y, Katayama N, Takeuchi K, Togano T, Itoh R, Sato M, Yamazaki M, Abe M, Sato T, Oda K, Yokoyama M, et al.
The Journal of biological chemistry (2013) 28848: 34906-19. 105 002 WB; tested species: mouse

Unique luminal localization of VGAT-C terminus allows for selective labeling of active cortical GABAergic synapses.
Martens H, Weston MC, Boulland JL, Grønborg M, Grosche J, Kacza J, Hoffmann A, Matteoli M, Takamori S, Harkany T, Chaudhry FA, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2849: 13125-31. 105 002 WB; tested species: rat

Synaptotagmin I and II are present in distinct subsets of central synapses.
Fox MA, Sanes JR
The Journal of comparative neurology (2007) 5032: 280-96. 105 002 WB; tested species: zebrafish

Borna disease virus blocks potentiation of presynaptic activity through inhibition of protein kinase C signaling.
Volmer R, Monnet C, Gonzalez-Dunia D
PLoS pathogens (2006) 23: e19. 105 002 ICC, WB; tested species: rat

SV2B regulates synaptotagmin 1 by direct interaction.
Lazzell DR, Belizaire R, Thakur P, Sherry DM, Janz R
The Journal of biological chemistry (2004) 27950: 52124-31. 105 002 WB; tested species: mouse

Microglia-synapse engulfment via PtdSer-TREM2 ameliorates neuronal hyperactivity in Alzheimer's disease models.
Rueda-Carrasco J, Sokolova D, Lee SE, Childs T, Jurčáková N, Crowley G, De Schepper S, Ge JZ, Lachica JI, Toomey CE, Freeman OJ, et al.
The EMBO journal (2023) 4219: e113246. 105 002 ICC; tested species: rat

Developmental control of synaptic receptivity.
Barker AJ, Koch SM, Reed J, Barres BA, Ullian EM
The Journal of neuroscience : the official journal of the Society for Neuroscience (2008) 2833: 8150-60. 105 002 ICC; tested species: rat

Implication of synaptotagmins 4 and 7 in activity-dependent somatodendritic dopamine release in the ventral midbrain.
Delignat-Lavaud B, Ducrot C, Kouwenhoven W, Feller N, Trudeau LÉ
Open biology (2022) 123: 210339. 105 002 IHC; tested species: mouse

Critical role of JSAP1 and JLP in axonal transport in the cerebellar Purkinje cells of mice.
Sato T, Ishikawa M, Yoshihara T, Nakazato R, Higashida H, Asano M, Yoshioka K
FEBS letters (2015) 58919 Pt B: 2805-11. 105 002 IHC

Dishevelled proteins are associated with olfactory sensory neuron presynaptic terminals.
Rodriguez-Gil DJ, Hu W, Greer CA
PloS one (2013) 82: e56561. 105 002 IHC; tested species: mouse

Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 105 002 DNA-PAINT; tested species: rat

Cat. No.: 105 002

Amount: 200 µl

Price: $355.00

Synaptotagmin I and II are present in distinct subsets of central synapses.
Fox MA, Sanes JR
The Journal of comparative neurology (2007) 5032: 280-96. 105 002 WB; tested species: zebrafish

Dishevelled proteins are associated with olfactory sensory neuron presynaptic terminals.
Rodriguez-Gil DJ, Hu W, Greer CA
PloS one (2013) 82: e56561. 105 002 IHC; tested species: mouse

ICC

IHC

IHC-P

Background

Synaptotagmin1 also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca²⁺-binding C2-domains.
Ca²⁺-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca²⁺-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.

1 ) Geppert M et al., Annu. Rev. Neurosci. (1998)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Südhof TC et al., Nature (1995)
4 ) Geppert M et al., Cell (1994)
5 ) Brose N et al., Science (1992)
6 ) Perin MS et al., Nature (1990)

Protocols

Standard protocol

ICC

IHC

IHC-P

ELISA

Membrane proteins

OTHER

Preabsorbtion

Other products for this target

Synaptotagmin1/2 antibody cytoplasmic tail - 105 002

References for Synaptotagmin1/2 - 105 002