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Synaptotagmin1 peptide luminal domain - 105-1P

Synaptotagmin1 is a Ca2+-sensor on synaptic vesicles that triggers neurotransmitter release
control peptide
Cat. No.: 105-1P
Amount: 100 µg
Price: $105.00
Cat. No. 105-1P 100 µg peptide, lyophilized. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Control peptides should be stored at -20°C when still lyophilized!
Applications
 
Immunogen Synthetic peptide corresponding to AA 1 to 19 from rat Synaptotagmin1 (UniProt Id: P21707)
Recommended dilution Optimal concentrations should be determined by the end-user.
Remarks

This control peptide consists of the synthetic peptide (aa 1 - 19 of rat synaptotagmin 1) that has been used for immunization. It has been tested in preadsorption experiments and blocks efficiently and specifically the corresponding signal in Western blots. The amount of peptide needed for efficient blocking depends on the titer and on the affinity of the antibody to the antigen.

Data sheet 105-1p.pdf
Cat. No.: 105-1P
Amount: 100 µg
Price: $105.00
Background

Synaptotagmin1, also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.

Protocols