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Endobrevin - 104 303

Endosomal fusion protein, member of the R-SNARE family
Polyclonal rabbit purified antibody
Cat. No.: 104 303
Amount: 50 µg
Price: $450.00
Cat. No. 104 303 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 100 up to 1 : 500 gallery  
IHC: yes
IHC-P/FFPE: not tested yet
Immunogen Recombinant protein corresponding to AA 1 to 75 from rat Endobrevin (UniProt Id: Q9WUF4)
Reactivity Reacts with: human (Q9BV40), rat (Q9WUF4), mouse (O70404).
Other species not tested yet.
Specificity Specific for endobrevin. K.O. PubMed: 25138214
Data sheet 104_303.pdf

References for Endobrevin - 104 303

Defective AP-3-dependent VAMP8 trafficking impairs Weibel-Palade body exocytosis in Hermansky-Pudlak Syndrome type 2 blood outgrowth endothelial cells.
Karampini E, Schillemans M, Hofman M, van Alphen F, de Boer M, Kuijpers TW, van den Biggelaar M, Voorberg J, Bierings R
Haematologica (2019) : . 104 303 WB, ICC; KD verified; tested species: human
Syntaxin 8 regulates platelet dense granule secretion, aggregation, and thrombus stability.
Golebiewska EM, Harper MT, Williams CM, Savage JS, Goggs R, Fischer von Mollard G, Poole AW
The Journal of biological chemistry (2015) 2903: 1536-45. 104 303 WB
Vesicle associated membrane protein 8 (VAMP8)-mediated zymogen granule exocytosis is dependent on endosomal trafficking via the constitutive-like secretory pathway.
Messenger SW, Falkowski MA, Thomas DD, Jones EK, Hong W, Gaisano HY, Giasano HY, Boulis NM, Groblewski GE
The Journal of biological chemistry (2014) 28940: 28040-53. 104 303 WB, IHC; KO verified
Defective AP-3-dependent VAMP8 trafficking impairs Weibel-Palade body exocytosis in Hermansky-Pudlak Syndrome type 2 blood outgrowth endothelial cells.
Karampini E, Schillemans M, Hofman M, van Alphen F, de Boer M, Kuijpers TW, van den Biggelaar M, Voorberg J, Bierings R
Haematologica (2019) : . 104 303 WB, ICC; KD verified; tested species: human
Fluorescence Lifetime Imaging Microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation.
Verboogen DRJ, González Mancha N, Ter Beest M, van den Bogaart G
eLife (2017) 6: . 104 303 ICC; tested species: human
Vesicle associated membrane protein 8 (VAMP8)-mediated zymogen granule exocytosis is dependent on endosomal trafficking via the constitutive-like secretory pathway.
Messenger SW, Falkowski MA, Thomas DD, Jones EK, Hong W, Gaisano HY, Giasano HY, Boulis NM, Groblewski GE
The Journal of biological chemistry (2014) 28940: 28040-53. 104 303 WB, IHC; KO verified
Cat. No.: 104 303
Quantity: 50 µg
Price: $450.00
Defective AP-3-dependent VAMP8 trafficking impairs Weibel-Palade body exocytosis in Hermansky-Pudlak Syndrome type 2 blood outgrowth endothelial cells.
Karampini E, Schillemans M, Hofman M, van Alphen F, de Boer M, Kuijpers TW, van den Biggelaar M, Voorberg J, Bierings R
Haematologica (2019) : . 104 303 WB, ICC; KD verified; tested species: human
Syntaxin 8 regulates platelet dense granule secretion, aggregation, and thrombus stability.
Golebiewska EM, Harper MT, Williams CM, Savage JS, Goggs R, Fischer von Mollard G, Poole AW
The Journal of biological chemistry (2015) 2903: 1536-45. 104 303 WB
Vesicle associated membrane protein 8 (VAMP8)-mediated zymogen granule exocytosis is dependent on endosomal trafficking via the constitutive-like secretory pathway.
Messenger SW, Falkowski MA, Thomas DD, Jones EK, Hong W, Gaisano HY, Giasano HY, Boulis NM, Groblewski GE
The Journal of biological chemistry (2014) 28940: 28040-53. 104 303 WB, IHC; KO verified
Defective AP-3-dependent VAMP8 trafficking impairs Weibel-Palade body exocytosis in Hermansky-Pudlak Syndrome type 2 blood outgrowth endothelial cells.
Karampini E, Schillemans M, Hofman M, van Alphen F, de Boer M, Kuijpers TW, van den Biggelaar M, Voorberg J, Bierings R
Haematologica (2019) : . 104 303 WB, ICC; KD verified; tested species: human
Fluorescence Lifetime Imaging Microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation.
Verboogen DRJ, González Mancha N, Ter Beest M, van den Bogaart G
eLife (2017) 6: . 104 303 ICC; tested species: human
Vesicle associated membrane protein 8 (VAMP8)-mediated zymogen granule exocytosis is dependent on endosomal trafficking via the constitutive-like secretory pathway.
Messenger SW, Falkowski MA, Thomas DD, Jones EK, Hong W, Gaisano HY, Giasano HY, Boulis NM, Groblewski GE
The Journal of biological chemistry (2014) 28940: 28040-53. 104 303 WB, IHC; KO verified
Background

Endobrevin/VAMP8, a member of the SNARE family of proteins, is a relative of synaptobrevin that is involved in the fusion of early and late endosomes. Endobrevin is expressed in most mammalian cells but appears to be absent from neurons. The protein is predominantly localized to early and late endosomal membranes but is also found on other membranes and organelles involved in endocytic membrane traffic. In the fusion of late endosomes it forms SNARE complexes with syntaxin 7, syntaxin 8 and vti1b.