Cat. No.: 104 103
Amount: 50 µg
|Cat. No. 104 103||
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: yes
Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: yes
Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: yes
Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 200 gallery
Electron microscopy (EM) is a microscopy technique that detects the scatter of electrons through thin tissue sections. In Immuno-EM the antigen is usually revealed by colloidal gold conjugated secondary antibodies linking electron dense structures to antigen-bound primary antibodies.', $event)" style="cursor: help;">EM: yes
|Immunogen||Synthetic peptide corresponding to AA 2 to 14 from rat Cellubrevin (UniProt Id: P63025)|
Reacts with: rat (P63025), mouse (P63024), pig.
No signal: human, zebrafish.
Other species not tested yet.
|Specificity||K.O. validated PubMed: 25864578|
|Matching control protein/peptide||104-1P|
Cellubrevin/VAMP3 is the non-neuronal isoform of the synaptobrevin/VAMP family which belongs to the v-SNAREs. Like the neuronal isoforms Synaptobrevin1/VAMP1 and Synaptobrevin2/VAMP2 it is composed of an N-terminal cytoplasmic region and a C-terminal transmembrane domain.
Cellubrevin/VAMP3 localizes to an endosomal membrane pool, where it constitutes an essential component of the membrane fusion machinery. Like the synaptobrevins, cellubrevin is a substrate for the tetanus toxin.