Synaptic Systems - Synaptogyrin3

Cat. No.: 103 302

Amount: 200 µl

Price: $355.00

Cat. No. 103 302	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 216 to 229 from mouse Synaptogyrin3 (UniProt Id: Q8R191)
Reactivity	Reacts with: mouse (Q8R191). Other species not tested yet. No signal on rat Synaptogyrin 3 (D4ABK1). Detects rat Synaptogyrin 3 isoform X1 (XP_006246009.2)
Specificity	K.O. validated PubMed: 31090538
Matching control protein/peptide	103-3P
Data sheet	103_302.pdf

References for Synaptogyrin3 - 103 302

Mutations in Parkinsonism-linked endocytic proteins synaptojanin1 and auxilin have synergistic effects on dopaminergic axonal pathology.
Ng XY, Wu Y, Lin Y, Yaqoob SM, Greene LE, De Camilli P, Cao M
NPJ Parkinson's disease (2023) 91: 26. 103 302 WB; tested species: mouse

Elevated synaptic vesicle release probability in synaptophysin/gyrin family quadruple knockouts.
Raja MK, Preobraschenski J, Del Olmo-Cabrera S, Martinez-Turrillas R, Jahn R, Perez-Otano I, Wesseling JF
eLife (2019) 8: . 103 302 WB; KO verified; tested species: mouse

Cat. No.: 103 302

Amount: 200 µl

Price: $355.00

ICC

IHC

IHC-P

Background

Synaptogyrins are tyrosine-phosphorylated proteins with two neuronal (synaptogyrin 1 and 3) and one ubiquitous, synaptogyrin 2 or cellugyrin isoform.
Synaptogyrins are integral membrane proteins and localize to the membrane of small vesicles. Synaptogyrin 1 and 3 are expressed in the brain whereby the latter shows a more restricted expression pattern with high levels in the mossy fiber region of the hippocampus, substantia nigra pars reticulata, pallidum, and deep cerebellar nuclei.
Synaptogyrin 2/cellugyrin, a close relative, is expressed in all tissues, for instance, in distinct populations of GLUT 4 containing vesicles.

1 ) Janz R et al., Neuron (1999)
2 ) Jahn R et al., Annu. Rev. Neurosci. (1994)
3 ) Janz R et al., J. Biol. Chem. (1998)
4 ) Südhof TC et al., Nature (1995)
5 ) Egaña LA et al., J. Neurosci. (2009)
6 ) Belizaire R et al., J. Comp. Neurol. (2004)

Protocols

ICC

IHC

IHC-P

OTHER

Preabsorbtion

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Synaptogyrin3 antibody - 103 302 K.O.

References for Synaptogyrin3 - 103 302