|Cat. No. 102 011||100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.|
WB: 1 : 1000 AP staining gallery
IP: not tested yet
ICC: 1 : 500 gallery
IHC: 1 : 1000 up to 1 : 2000 (see remarks) gallery
IHC-P/FFPE: 1 : 1000 gallery
|Subtype||IgG2b (κ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 250 to 263 from rat Synaptoporin (UniProt Id: P22831)|
Epitop: AA 250 to 263 from rat Synaptoporin (UniProt Id: P22831)
Reacts with: mouse (Q8BGN8), rat (P22831).
Other species not tested yet.
|Specificity||Specific for synaptoporin.|
|Matching control protein/peptide||102-1P|
IHC: In addition to the expected strong mossy fibre staining, this antibody shows some weak unspecific staining of cell bodies in the cortex, striatum and cerebellum.
Synaptoporin, also known as synaptophysin 2 and p38-2, is highly homologous to synaptophysin 1 but encoded by a different gene. Like synaptopysin 1, synaptoporin contains four transmembrane regions and a short cytoplasmic tail. Unlike synaptophysin 1, it is not glycosylated.
The distributions of synaptophysin 1 and synaptoporin are different. Synaptophysin 1 is more uniformly expressed whereas synaptoporin is particularly enriched in mossy fiber synapses in the hippocampus. It is thus an excellent marker for subsets of synapses.