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Home > Products > 101 111

Synaptophysin1/2 antibody - 101 111

Synaptophysins are major synaptic vesicle membrane protein
Mouse monoclonal purified IgG
Cat. No.: 101 111
Amount: 100 µg
Price: $415.00
Cat. No. 101 111 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 AP staining (see remarks) gallery  
IP: yes (see remarks)
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 200 up to 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone 318H7
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 250 to 265 from rat Synaptoporin (UniProt Id: P22831)
Reactivity Reacts with: mouse (Q8BGN8, Q62277), rat (P22831, P07825).
Other species not tested yet.
Specificity Recognizes Synaptophysin1 and 2 (Synaptoporin) with strong preference for Synaptophysin1 in Western blot.
Matching control protein/peptide 102-0P
Remarks

WB: Shows a strong preference for Synaptophysin 1
IP: Shows strong preference for Synaptophysin 1.
Data sheet 101_111.pdf

References for Synaptophysin1/2 - 101 111

Evolving prion-like tau conformers differentially alter postsynaptic proteins in neurons inoculated with distinct isolates of Alzheimer's disease tau.
Hromadkova L, Kim C, Haldiman T, Peng L, Zhu X, Cohen M, de Silva R, Safar JG
Cell & bioscience (2023) 131: 174. 101 111 WB; tested species: mouse
Cat. No.: 101 111
Amount: 100 µg
Price: $415.00
Evolving prion-like tau conformers differentially alter postsynaptic proteins in neurons inoculated with distinct isolates of Alzheimer's disease tau.
Hromadkova L, Kim C, Haldiman T, Peng L, Zhu X, Cohen M, de Silva R, Safar JG
Cell & bioscience (2023) 131: 174. 101 111 WB; tested species: mouse
WB
ICC
IHC
IHC
IHC
IHC-P
Background

Synaptophysin1, also referred to as p38-1, is a membrane glycoprotein of synaptic vesicles that is ubiquitously expressed in all neurons and in many endocrine cells. It is currently the most widely used marker for nerve terminals and probably the best marker for the pathologist in differentiating neuroendocrine tumors.
Synaptophysin1 has four transmembrane domains with both N- and C-terminus facing the cytoplasm. It binds to synaptobrevin1 and synaptobrevin2 in detergent extracts but its function has not been elucidated completely. It forms a complex with dynamin at high Ca2+ concentration suggesting an involvement in synaptic vesicle endocytosis. As typical for synaptic vesicle proteins, synaptophysin 1 represents a small protein family with two additonal members, synaptoporin and panthophysin.
Synaptoporin, also known as synaptophysin2 and p38-2, is highly homologous to synaptophysin1 but encoded by a different gene. Like synaptopysin1, synaptoporin contains four transmembrane regions and a short cytoplasmic tail. Unlike synaptophysin1, it is not glycosylated.
The distributions of synaptophysin1 and synaptoporin are different. Synaptophysin1 is more uniformly expressed whereas synaptoporin is particularly enriched in mossy fiber synapses in the hippocampus. It is thus an excellent marker for subsets of synapses.