Tailor-made Antibodies
and Tools for Life Science

Synaptophysin1 antibody - 101 009 K.O.

Synaptophysin is a major synaptic vesicle membrane protein and a useful neuroendocrine tumor marker
Chicken monoclonal recombinant IgY
Cat. No.: 101 009
Amount: 50 µg
Price: $420.00
Cat. No. 101 009 50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 2000 (AP-staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 2000 gallery  
Clone Ch7.2
Subtype IgY (λ light chain)
Immunogen Full-length recombinant rat synaptophysin (UniProt Id: P07825)
Epitop AA 219 to 307 from rat Synaptophysin1 (UniProt Id: P07825)
Reactivity Reacts with: human (P08247), rat (P07825), mouse (Q62277), other mammals.
Weaker signal: zebrafish, other vertebrates.
Other species not tested yet.
Specificity K.O. validated
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 7.2. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. Therefore, the antibody can be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.
Synaptophysin1 is widely used as marker for nerve terminals and neuroendocrine tumors. For still unknown reason, neuronal synaptophysin is better recognized than neuroendocrine synaptophysin. If this is a problem, the polyclonal rabbit antibody, cat. no. 101 002 or 101 203, is recommended.

Data sheet 101_009.pdf
Cat. No.: 101 009
Amount: 50 µg
Price: $420.00