Synaptic Systems

Cat. No.: 263 004

Amount: 100 µl

Price: $365.00

Cat. No. 263 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 200 up to 1 : 500 gallery IHC-P: 1 : 200 up to 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 427 from rat Giantin (UniProt Id: G3V6A8)
Reactivity	Reacts with: rat, mouse. Other species not tested yet.
Remarks	WB: Due to its large size, Giantin requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels from anamed or NuPAGE 3-8% TRIS-Acetate gradient gels from invitrogen.
Data sheet	263_004.pdf

References for Giantin - 263 004

Golgi satellites are essential for polysialylation of NCAM and expression of LTP at distal synapses.
Andres-Alonso M, Borgmeyer M, Mirzapourdelavar H, Lormann J, Klein K, Schweizer M, Hoffmeister-Ullerich S, Oelschlegel AM, Dityatev A, Kreutz MR
Cell reports (2023) 427: 112692. 263 004 ICC, IHC; tested species: mouse

Regulation of dendrite morphology and excitatory synapse formation by zDHHC15.
Shah BS, Shimell JJ, Bamji SX
Journal of cell science (2019) 13213: . 263 004 ICC; tested species: rat

Cat. No.: 263 004

Amount: 100 µl

Price: $365.00

Regulation of dendrite morphology and excitatory synapse formation by zDHHC15.
Shah BS, Shimell JJ, Bamji SX
Journal of cell science (2019) 13213: . 263 004 ICC; tested species: rat

ICC

IHC

IHC-P

Background

In eukaryotic cells the Golgi complex consists of stacked cisternae. Giantin, also referred to as macrogolgin or p400, is a single pass membrane protein involved in tethering these cisternae by cross-bridge formation. Its large cytoplasmic N-terminus is involved in the docking of COPI vesicles via p115 to the Golgi membrane.
Systemic lupus erythematosus and Sjögren's syndrome correlate with autoimmune antibodies against Golgi-compartment proteins with giantin being the most common autoantigen.

1 ) Rosing M et al., Exp. Cell Res. (2007)
2 ) Nozawa K et al., Arthritis Res. Ther. (2004)
3 ) Alvarez C et al., J. Biol. Chem. (2001)
4 ) Sönnichsen B et al., J. Cell Biol. (1998)
5 ) Seelig HP et al., Mol. Cell. Biol. (1994)
6 ) Linstedt AD et al., Mol. Biol. Cell (1993)

Protocols

ICC

IHC

IHC-P

Other products for this target

Giantin antibody - 263 004

References for Giantin - 263 004