Synaptic Systems

Cat. No.: 355 002

Amount: 200 µl

Price: $360.00

Cat. No. 355 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P (FFPE): 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Full length mouse recombinant CNP1 (UniProt Id: P16330)
Reactivity	Reacts with: rat (P13233), mouse (P16330). Other species not tested yet.
Specificity	K.O. validated
Data sheet	Datasheet 355_002

References for CNP1 - 355 002

Rab6A as a Pan-Astrocytic Marker in Mouse and Human Brain, and Comparison with Other Glial Markers (GFAP, GS, Aldh1L1, SOX9).
Melzer L, Freiman TM, Derouiche A
Cells (2021) 101: . 355 002 IHC; tested species: mouse

Immunohistochemical analysis of spinal cord components in mouse model of experimental autoimmune encephalomyelitis.
Pyka-Fosciak G, Stasiolek M, Litwin JA
Folia histochemica et cytobiologica (2018) 563: 151-158. 355 002 IHC; tested species: mouse

Cat. No.: 355 002

Amount: 200 µl

Price: $360.00

Background

The 2', 3'-cyclic nucleotide 3'-phosphodiesterase CNP1, also referred to as CNPase and CNP, is one of the most abundant membrane-associated enzymes in the myelin sheath of the vertebrate nervous system.
It is assumed that CNP1 participates in RNA metabolism of myelinating oligodendrocytes.

1 ) Myllykoski M et al., PLoS ONE (2012)
2 ) Edgar NM et al., Transl Psychiatry (2011)
3 ) Lappe-Siefke C et al., Nat. Genet. (2003)
4 ) Brunner C et al., J. Neurochem. (1989)
5 ) Braun PE et al., J. Neurosci. (1988)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

Standard Condition

Other products for this target

CNP1 (CNPase) antibody - 355 002 K.O.

References for CNP1 - 355 002