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mRuby sdAb - FluoTag-X2 - N3302-Ab580-L

Single domain antibody recognizing a pH resistant fluorescent protein

This product was developed by   

NanoTag-Biotechnologies

FluoTags are camelid single domain antibodies consisting only of one antigen binding site of a Alpaka heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Single Domain

Cat. No.: N3302-Ab580-L
Amount: 200 µl
Price: $485.00
Cat. No. N3302-Ab580-L

2.5 µM fluorescently labeled sdAb in buffered saline, 50% glycerol, 0.09% sodium azide

Storage –80°C for up to 12 month
Applications WB: not recommended
IP: N/A
ICC: 1 : 500
IHC: not tested yet
IHC-P/FFPE: not tested yet
Label AbberiorStar 580, two fluorophores coupled to one FluoTag
Clone 1F8
Immunogen Recombinant protein corresponding to AA 1 to 227 from Entacmaea quadricolor mRubymRUBY3
Specificity Specific for mRUBY3 and mRUBY derivatives
Documents data sheet

Cat. No.: N3302-Ab580-L
Quantity: 200 µl
Price: $485.00
Background

 mRuby and mRuby derivatives are bright monomeric red fluorescent proteins derived from Entacmaea quadricolar. They show exceptional resistance to denaturation at pH extremes.


In FluoTag-X two fluorophore molecules are site-specifically coupled to each FluoTag molecule which ensures bright signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is below 4 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.

Protocols