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FluoTags are camelid single domain antibodies consisting only of one antigen binding site of a Alpaka heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
|Cat. No. N3202-Ab635P-L||
2.5 µM fluorescently labeled sdAb in buffered saline, 50% glycerol, 0.09% sodium azide
|Storage||–80°C for up to 12 month|
WB: not recommended
ICC: 1 : 500
IHC: not tested yet
IHC-P/FFPE: not tested yet
|Label||AbberiorStar 635P, two fluorophores coupled to one FluoTag|
|Specificity||Specific for mNeonGreen|
mNeonGreen is a bright monomeric green fluorescent protein derived from Branchiostoma lanceolatume. It is approximatly three times brighter compared to EGFP.
In FluoTag-X two fluorophore molecules are site-specifically coupled to each FluoTag molecule which ensures bright signals. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is below 4 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.