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Synaptotagmin 1 sdAb cytoplasmic tail - FluoTag-X2 - N2302-At565-L

Ca2+-sensor on synaptic vesicles, triggers neurotransmitter release

 

This product was developed by   

NanoTag-Biotechnologies

FluoTags are camelid single domain antibodies consisting only of one antigen binding site of a Alpaka heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Single Domain

Cat. No.: N2302-At565-L
Amount: 200 µl
Price: 455.00 €
Cat. No. N2302-At565-L

200µl 2.5µM camelid sdAb in buffered saline, 50% glycerol, 0.09% sodium azide

Storage Up to 3 months: –20 °C
Up to 12 months: –80 °C or below
Protect from light!
Applications ICC: 1 : 500 gallery  
IHC: 1 : 500
Label ATTO 565, two fluorophores coupled to one FluoTag
Clone 7D5
Subtype single domain
Immunogen Recombinant protein corresponding to AA 80 to 421 from rat Synaptotagmin1 (UniProt Id: P21707)
Reactivity Reacts with: mouse (P46096), rat (P21707).
Other species not tested yet.
Specificity Specific for Synaptotagmin 1
Documents Data sheet.pdf

Cat. No.: N2302-At565-L
Quantity: 200 µl
Price: 455.00 €
Background

Synaptotagmin 1 also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.