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FluoTags are camelid single domain antibodies consisting only of one antigen binding site of a Alpaka heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.
|Cat. No. N2302-At488-S||
20µl 2.5µM camelid sdAb in buffered saline, 50% glycerol, 0.09% sodium azide
Up to 3 months: –20 °C
Up to 12 months: –80 °C or below
Protect from light!
ICC: 1 : 500 gallery
IHC: 1 : 500
|Label||ATTO 488, two fluorophores coupled to one FluoTag|
|Immunogen||Recombinant protein corresponding to AA 80 to 421 from rat Synaptotagmin1 (UniProt Id: P21707)|
Reacts with: mouse (P46096), rat (P21707).
Other species not tested yet.
|Specificity||Specific for Synaptotagmin 1|
Synaptotagmin 1 also known as p65, is an integral membrane glycoprotein of neuronal synaptic vesicles and secretory granules of neuroendocrine cells that is widely (but not ubiquitously) expressed in the central and peripheral nervous system. It has a variable N-terminal domain that is exposed to the lumen of the vesicle and a conserved cytoplasmic tail that contains two Ca2+-binding C2-domains.
Ca2+-binding to synaptotagmin triggers exocytosis of synaptic vesicles, thus linking Ca2+-influx during depolarization to neurotransmitter release.
Lumenal antibodies were used in living neurons to label synaptic vesicles from the outside via endocytotic uptake.