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VGluT 1 sdAb - FluoTag-X2 - N1602-SC5-L

Glutamate transporter in the membrane of synaptic vesicles

 

This product was developed by   

NanoTag-Biotechnologies

FluoTags are camelid single domain antibodies consisting only of one antigen binding site of a Alpaka heavy chain antibody. With only ~15 kDa, these Tags are about 10-times smaller than conventional IgG antibody molecules.

Single Domain

Cat. No.: N1602-SC5-L
Amount: 200 µl
Price: $485.00
Cat. No. N1602-SC5-L

200µl 5µM camelid sdAb in buffered saline, 50% glycerol, 0.09% sodium azide

Applications WB: not recommended
IP: N/A
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P/FFPE: not tested yet
Label Sulfo-Cyanine 5, two fluorophores coupled to one FluoTag
Clone Nb9
Subtype single domain
Immunogen Recombinant protein corresponding to AA 58 to 515 from rat VGLUT1 (UniProt Id: Q62634)
Epitop Epitop: AA 58 to 515 from rat VGLUT1 (UniProt Id: Q62634)
Reactivity Reacts with: rat (Q62634), mouse (Q3TXX4).
Other species not tested yet.
Specificity Specific for VGLUT 1
Documents data sheet

References for VGluT 1 sdAb - N1602-SC5-L

Generation and Characterization of Anti-VGLUT Nanobodies Acting as Inhibitors of Transport.
Schenck S, Kunz L, Sahlender D, Pardon E, Geertsma ER, Savtchouk I, Suzuki T, Neldner Y, Štefanić S, Steyaert J, Volterra A, et al.
Biochemistry (2017) 5630: 3962-3971. N1602-SC5-L ICC; tested species: mouse

Cat. No.: N1602-SC5-L
Quantity: 200 µl
Price: $485.00
Generation and Characterization of Anti-VGLUT Nanobodies Acting as Inhibitors of Transport.
Schenck S, Kunz L, Sahlender D, Pardon E, Geertsma ER, Savtchouk I, Suzuki T, Neldner Y, Štefanić S, Steyaert J, Volterra A, et al.
Biochemistry (2017) 5630: 3962-3971. N1602-SC5-L ICC; tested species: mouse
Background

The vesicular glutamate transporter 1 VGLUT 1, also referred to as BNPI and SLC17A7, was originally identified as a brain specific phosphate transporter. Like the related VGLUT 2, VGLUT 1 is both necessary and sufficient for uptake and storage of glutamate and thus comprises the sole determinant for a glutamatergic phenotype. Both VGLUTs are different from the plasma membrane transporters in that they are driven by a proton electrochemical gradient across the vesicle membrane.
VGLUT 1 and VGLUT 2 show complementary expression patterns. Together, they are currently the best markers for glutamatergic nerve terminals and glutamatergic synapses.

 

In FluoTag-X two fluorophore molecules are site-specifically coupled to each FluoTag molecule. Owing to the small size of the FluoTags, the distance between the target epitope and each fluorophore is below 4 nm.
In comparison to detection systems using conventional antibodies, FluoTag-X can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render the FluoTag-X products excellent tools for all microscopy techniques.